Validation of Methods for Assessment of Dust Levels in Layer Barns.

The dust level is included in the animal welfare legislation of the European Union, implying assessment of dust levels during veterinary welfare inspections. This study aimed to develop a valid and feasible method for measuring dust levels in poultry barns. Dust levels were assessed in 11 layer barns using six methods: light scattering measurement, the dust sheet test with durations of 1 h and 2-3 h, respectively, visibility assessment, deposition assessment, and a tape test. As a reference, gravimetric measurements were obtained - a method known to be accurate but unsuitable for veterinary inspection. The dust sheet test 2-3 h showed the highest correlation with the reference method with the data points scattered closely around the regression line and the slope being highly significant (p = 0.00003). In addition, the dust sheet test 2-3 h had the highest adjusted R2 (0.9192) and the lowest RMSE (0.3553), indicating a high capability of predicting the true concentration value of dust in layer barns. Thus, the dust sheet test with a test duration of 2-3 h is a valid method for assessing dust levels. A major challenge is the test duration as 2-3 h is longer than most veterinary inspections. Nevertheless, results showed that potentially, with some modifications to the scoring scale, the dust sheet test may be reduced to 1 h without losing validity.

Positive Effects of Elevated Platforms and Straw Bales on the Welfare of Fast-Growing Broiler Chickens Reared at Two Different Stocking Densities.

In conventional rearing systems, fast-growing broiler chickens commonly experience welfare issues, such as contact dermatitis, walking difficulties or a lack of expression of species-specific behaviours. Enriching their environment may be a way to improve their welfare. The objective of this study was to evaluate the benefits of elevated platforms and straw bales on the welfare of fast-growing broiler chickens reared at two different stocking densities. A total of 14,994 Ross 308 broilers were housed in 12 pens according to 4 treatments: 31 kg/m2 with or without enrichments and 41 kg/m2 with or without enrichments. The broilers' walking ability, footpad dermatitis (FPD), hock burns (HB), weight, mortality and litter quality were assessed. Stocking density had a negative effect on FPD and HB, whereas enrichments reduced the occurrence of FPD and HB at both densities. There was a positive enrichment effect and a negative density effect on body weight at 25 days and on walking ability, but no effect on the litter quality or mortality rate. These results confirm that an enriched environment improves animal welfare in confined chickens, regardless of the stocking density. Reducing stocking density clearly appears to be an important means of increasing animal welfare.

Human behaviour at the origin of maternal effects on offspring behaviour in laying hens (Gallus gallus domesticus).

Regular visual presence of humans is known to reduce chickens' human-generated stress responses. Here we questioned whether, more than mere visual presence, human behaviour affects laying hen behaviour and subsequently their offspring's behaviour. We hypothesized that human behaviour triggers maternal effects via variations in yolk hormone levels. For five consecutive weeks, two groups of hens were exposed to the same durations of human presence (30 min twice a day, five days a week) but the behaviour of the human differed between groups. The first group (H+) was exposed to predictable arrival of the experimenter, slow movements combined with static presence, stroking during handling and human voice. Whereas the second group of hens (H-) was exposed to unpredictable arrival of the experimenter which remained silent, in motion, and did not provide stroking during handling. At the end of the treatment, we evaluated egg quality and offspring behaviour. We found that avoidance of the experimenter by H+ hens but not by H- hens decreased significantly. Fertility rates and concentrations of yolk progesterone and estradiol in H+ hens' eggs were higher than in H- hens' eggs. Fear of humans, neophobia or the capacity to solve a detour task did not differ significantly between H+ and H- chicks. Social discrimination tests showed that H+ chicks but not H- chicks typically preferred a familiar conspecific to a stranger. These results show that, with the same duration in the presence of the birds, humans through their behaviour engender variations in fertility rates, yolk hormone levels and transgenerational effects on social skills. Rarely explored, our data suggest that maternal effects influence filial imprinting. These data have broad implications for laboratory, commercial systems and conservatory programs where the inevitable presence of humans could trigger maternal effects on offspring phenotype.