Life cycle assessment of electric vehicles: a systematic review of literature.

This study addresses the pressing need to evaluate the life cycle assessment (LCA) of electric vehicles (EVs) in comparison to traditional vehicles, amid growing environmental concerns and the quest for sustainable transportation alternatives. Through a systematic four-stage literature review, it strives to provide essential insights into the environmental impact, energy consumption, and resource utilization associated with EVs, thereby informing well-informed decisions in the transition to more sustainable transportation systems. The study's findings underscore a compelling environmental advantage of EVs. They emit a staggering 97% less CO2 equivalent emissions when compared to petrol vehicles, and a significant 70% less compared to their diesel counterparts, rendering them a crucial instrument in the battle against climate change. These environmental benefits are intricately linked to the adoption of clean energy sources and advanced battery technology. Furthermore, the study highlights the potential for additional emissions reduction through the extension of EV lifespans achieved by recycling and advanced battery technologies, with Li-ion batteries enjoying a second life as secondary storage systems. However, challenges remain, most notably the scarcity of rare earth materials essential for EV technology. The study's policy recommendations advocate for a swift shift towards clean energy sources in both EV production and usage, substantial investments in advanced battery technology, and robust support for recycling initiatives. Addressing the rare earth material shortage is paramount to the sustained growth and viability of EVs, facilitating a greener and more sustainable future in the realm of transportation.

Lactiplantibacillus plantarum KAU007 Extract Modulates Critical Virulence Attributes and Biofilm Formation in Sinusitis Causing Streptococcus pyogenes.

Streptococcus pyogenes is one of the most common bacteria causing sinusitis in children and adult patients. Probiotics are known to cause antagonistic effects on S. pyogenes growth and biofilm formation. In the present study, we demonstrated the anti-biofilm and anti-virulence properties of Lactiplantibacillus plantarum KAU007 against S. pyogenes ATCC 8668. The antibacterial potential of L. plantarum KAU007 metabolite extract (LME) purified from the cell-free supernatant of L. plantarum KAU007 was evaluated in terms of minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). LME was further analyzed for its anti-biofilm potential using crystal violet assay and microscopic examination. Furthermore, the effect of LME was tested on the important virulence attributes of S. pyogenes, such as secreted protease production, hemolysis, extracellular polymeric substance production, and cell surface hydrophobicity. Additionally, the impact of LME on the expression of genes associated with biofilm formation and virulence attributes was analyzed using qPCR. The results revealed that LME significantly inhibited the growth and survival of S. pyogenes at a low concentration (MIC, 9.76 µg/mL; MBC, 39.06 µg/mL). Furthermore, LME inhibited biofilm formation and mitigated the production of extracellular polymeric substance at a concentration of 4.88 µg/mL in S. pyogenes. The results obtained from qPCR and biochemical assays advocated that LME suppresses the expression of various critical virulence-associated genes, which correspondingly affect various pathogenicity markers and were responsible for the impairment of virulence and biofilm formation in S. pyogenes. The non-hemolytic nature of LME and its anti-biofilm and anti-virulence properties against S. pyogenes invoke further investigation to study the role of LME as an antibacterial agent to combat streptococcal infections.

Global electric vehicle adoption: implementation and policy implications for India.

Present transport system of conventional vehicle in India has faced challenges due to enormous amount of air pollution, health hazards to human, rising oil price, insufficient indigenous fossil fuel reserve, heavy expenditure on oil import, energy insecurity, etc. Electrical vehicle (EV) is considered to be alternatives of conventional vehicles that can overcome these shortcomings. The aim of the study is to get an overview of the electric vehicle policies of government of India and its state governments to find out their relevance and impact on EV adoption in India. Exploratory research is used in present case to carry out the study. Currently, the EV industry in India is in preliminary condition and in growing stage. Government of India has framed policies such as "NEMMP 2020," "FAME-I," "FAME-II," and Vehicle Scrappage policy. Seventeen of its state governments have framed EV policy. These policies facilitated various types of incentives, infrastructure development, fund allocation, research and development, production, and sales. This will have strong impact on EV demand generation, conversion of conventional vehicles to e-vehicle resulting E-mobility transformation and EV hub in the region that is at par with other EV-developed countries in the world. However, lack of policy and technology availability in the domain of disposal and reprocessing of Li-ion battery is found to be a future limitation of EV prospect in Indian context which needs to be looked into.

Comparison of cubosomes and hexosomes for the delivery of phenytoin to the brain.

The ability to formulate cubosomes and hexosomes with a single lipid by changing only the colloidal stabiliser presents a unique opportunity to directly compare the biological performance of these uniquely structured nanoparticles. This was explored here via the encapsulation and brain delivery of a model anti-seizure drug, phenytoin, in selachyl alcohol cubosomes and hexosomes. Nanoparticles were prepared with Pluronic® F127 or Tween 80® as the stabiliser and characterised. The internal nanostructure of nanoparticles shifted from hexosomes when using Pluronic® F127 as the stabiliser to cubosomes when using Tween 80® and was conserved following loading of phenytoin, with high encapsulation efficiencies (>97%) in both particle type. Cytotoxicity towards brain endothelial cells using the hCMEC/D3 line was comparable regardless of stabiliser type. Finally, in vivo brain delivery of phenytoin encapsulated in cubosomes and hexosomes after intravenous administration to rats was studied over a period of 60 min, showing cubosomes to be superior to hexosomes, both in terms of brain concentrations and brain to plasma ratio. While the role of stabiliser and/or internal nanostructure remains to be conclusively determined, this study is the first in vivo comparison of cubosomes and hexosomes for the delivery of a therapeutic drug molecule across the BBB and into the brain.

Realigning India's Vietnam Policy Through Cooperative Sustainable Development: a Geostrategic Counterbalancing to China in Indo-Pacific.

Vietnam is a key player in India's Act East Policy and is distressed due to China's overarching position in the South China Sea. China's expanding infrastructural investments in India's periphery have led to a regional security dilemma in Indian Ocean Region. India is steered to pursue opportunities to counter China in the latter's periphery, to which Vietnam fits as an apt ally. Hence, this paper examines the heightened need for realigning India's Vietnam policy in line with United Nations Sustainable Development Goals and explains how bilateral cooperation through sustainable trade, renewable energy production, and green investments can offer a "counter" to Chinese expansion in Indo-Pacific and its Belt and Road Initiative. This paper uses the theoretical framework of Balance of Power to enumerate how geostrategic policy decisions in India-Vietnam bilateral relations can create a "counterbalance" to the Chinese investments in India's neighborhood, especially in Pakistan.

Assessment of energy vulnerability in urban crowded space of Indian Himalaya.

Energy poverty poses a serious threat to the urban population residing in Himalayan towns. The present study aims to assess energy vulnerability in the urban settlement of Indian Himalayan Region (IHR). The study addresses energy access and affordability; its requirement, practices, and efficiency, considering the quality of electricity, dwelling type, transport facilities, energy expenditure, household chores (cooking, heating, cooling, lighting, etc.), awareness, and social interactions. The study uses an energy vulnerability framework based on IPCC indices to investigate the status of energy vulnerability in the urban space of Dehradun, the Indian Himalayan Region. The primary data was collected from two circular regions of the city, an area close to the central place and an area outlying from the central place, respectively. The energy vulnerability index was 0.31 and 0.34 with varying degrees of exposure, sensitivity, and adaptive capacity. The findings reveal that energy vulnerability could jeopardize the well-being of the society and could be mitigated through appropriate policy measures, intensive support to sensitive societies, and a developmental approach focusing on sustainable energy. The study provides a theoretical base to conduct extensive research addressing a similar issue in other Himalayan towns.

Is geopolitics a threat for offshore wind energy? A case of Indian Ocean Region.

The aim of this paper is to drive the discourse towards the increasing shift to renewables, especially offshore wind energy generation, in the emerging international energy order. The Indian Ocean Region (IOR), despite its increasing contribution to onshore wind energy generation and impending policies on offshore wind energy, is reluctant to invest in the latter. Hence, this paper highlights four important aspects that challenge IOR's offshore wind energy development: Indian Ocean's strategic location, environment impacts, blue economy and maritime terrorism. In the background of the geopolitical rivalry existing in the Indian Ocean Region (IOR), with the increasing presence of China and the USA in the Indian Ocean, this paper aims to study if these geopolitical challenges are hindering offshore wind energy generation in IOR. The key findings of the paper include the necessity of addressing the geopolitical rivalry in IOR as an important hindrance in huge investments needed in OWE farms, so that a regional cooperative mechanism is arrived at especially from the point of view of policies towards OWE generation.

Valuing biodiversity attributes: a choice experiment design.

This study seeks to estimate household's willingness to pay for biodiversity conservation in Dachigam National Park, which houses the last viable population of Asiatic Black Bear and the Hangul (which is the only surviving sub-species of red deer in the world). A survey based on choice experiment method was carried out at Dachigam National Park, an area that is threatened by several anthropogenic pressures. A set of attributes, such as endangered species, national park area and research and education opportunities the park holds, have been selected to substantiate the analysis. In order to estimate willingness to pay (WTP), a monetary variable involving an increase in water rates was also incorporated. Willingness to pay for the selected attributes per household ranges from Rs. 109.48 to Rs. 138.48 for enhancing population of endangered species, Rs. 48.54 to Rs. 82.36 for improvement in park area and Rs. 67.21 to Rs. 101.35 for increasing research and education opportunities the park holds. Findings that the study present can be used as an indicator of economic importance of biological resources for their better management and conservation that can help in ensuring sustainable utilization of these natural resources.

Steric stabilisers govern the colloidal and chemical stability but not in vitro cellular toxicity of linoleoylethanolamide cubosomes.

Linoleoylethanolamide (LEA) is an endogenous lipid with remarkable neuromodulatory properties. However, its therapeutic potential is limited by rapid clearance in vivo, targetability and solubility. This study aimed to formulate LEA into liquid crystalline nanoparticles (cubosomes) as a strategy to address the aforementioned challenges. The influence of three different steric stabilisers: Tween 80 and Pluronic F68, both of which have the potential to interact with receptors expressed at the blood-brain barrier and Pluronic F127 as a control, on colloidal stability, internal structure, chemical stability and cytotoxicity of the dispersions were investigated. We found that for effective stabilization of LEA dispersions, a higher concentration of Tween 80 was required compared to Pluronics. Freshly prepared dispersions showed mean particle size of <250 nm and low PDIs (<0.2), with an Im3m type cubic structure but with different lattice parameters. Upon storage at ambient temperature for a week, increased mean particle size and PDI, with a significant reduction in the concentration of LEA was observed in Tween 80-stabilised dispersions. Greater than 80% cell viability was observed at concentrations of up to 20 µg/mL LEA in the presence of all three stabilisers. Collectively, our results suggest that the stabiliser type influences colloidal and chemical stability but not cytotoxicity of LEA cubosomes. This study highlights the potential of endogenous bioactive lipids to be utilized as core cubosome forming lipids with the view to improving their solubility, rapid clearance and targetability to enable delivery of these bioactive molecules to the brain.

Prevalence, molecular diagnosis and treatment of Mycoplasma conjunctivae isolated from infectious keratoconjunctivitis affected Lohi sheep maintained at Livestock Experiment Station, Bahadurnagar, Okara, Pakistan.

Mycoplasma conjunctivae are etiological agents of infectious keratoconjunctivitis (IKC), commonly known as pink-eye in domestic sheep, goats and other wild animals in many parts of the world. A few young Lohi lambs maintained at Livestock Experiment Station (LES), Bahadurnagar, Okara, Pakistan showed clinical signs and symptoms of conjunctivitis, keratitis, severe lacrimation and varying degree of blindness. During January to March, 2011, a total of 36 ocular swabs were collected from IKC affected animals and were processed for isolation, identification, and characterization of M. conjunctivae. Sixteen (44.44 %) out of 36 samples showed turbidity in PPLO broth. Twelve (75 %) out of 16 broth samples showed colony growth on PPLO agar. All 16 (44.44 %) out of 36 turbid broth samples, 12 (75 %) out of 16 cultured on agar plate samples, and 21 (59 %) out of 36 sheep ocular direct swab samples were found positive for M. conjunctivae through polymerase chain reaction test by using M. conjunctivae-specific primer pair McoF1 and McoR1 and detecting a 750 base pair fragment on agarose gel. Topical application of 0.5 % sterile solution of gentamycin (100 mg/ml) (Gentafar 10 %, FARVET, Netherlands) proved suitable for the treatment of IKC in Lohi lambs as all clinical signs of IKC disappeared after 5 days of treatment with this antibiotic. This is the first report about the prevalence, molecular diagnosis, and treatment of M. conjunctivae in Lohi sheep affected with infectious keratoconjunctivitis at LES, Bahadurnagar, Okara, Pakistan.