RESUMO
miRNAs (MicroRNAs), known as noncoding and important endogenous factors regulating the expression protein-coding genes, are vital regulators in each biological process. Thus, this study aims to explore the key role of four microRNAs in regulating the spermatogenesis. To conduct this experiment, 55 infertile and fertile men provided the study with the sperm and testicular tissue samples. To study the spermatozoa in terms of the morphology, Diff-Quick was applied. Then, quantitative real-time polymerase chain reaction (RT-PCR) was conducted on samples. Our data indicated that in contrast to the miR-15b, significant increasing of miR-383 and miR-122 occurred in both severe oligoasthenoteratozoospermia (SOAT) and moderate oligoasthenoteratozoospermia (MOAT) compared to normal sperm group (N). In addition, it was observed that miR-15b and miR-122 increased in patients with nonobstructive azoospermia (NOA) compared with obstructive azoospermia (OA) group. Expression levels of target genes including P53, CASPASE-9 and CYCLIN D1 underwent principle changes according to miRNAs expression level. Our finding indicated that miRNAs had essential role in the regulation of spermatogenesis, and their expression altering was associated with sperm abnormalities. Thus, microRNAs can be introduced as useful biomarkers to determine male infertility reasons to choose the effective treatment.
Assuntos
Azoospermia/diagnóstico , Redes Reguladoras de Genes , MicroRNAs/metabolismo , Oligospermia/diagnóstico , Espermatogênese/genética , Adulto , Azoospermia/genética , Biomarcadores/análise , Biomarcadores/metabolismo , Caspase 9/genética , Ciclina D1/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Humanos , Masculino , MicroRNAs/análise , Oligospermia/genética , Espermatozoides/metabolismo , Proteína Supressora de Tumor p53/genética , Adulto JovemRESUMO
Genetic analyses, structure, and bottlenecks were examined in six populations of Iranian indigenous goat using 13 microsatellite loci. The overall heterozygosity, polymorphism information content (PIC), and Shannon index values were 0.80, 0.74, and 2.14, respectively, indicating high genetic diversity. Both a phylogenetic tree and factorial correspondence analysis grouped the populations into two major clusters. Signatures for bottleneck events in the populations were examined by two methods, which suggested that bottlenecks had occurred in two Tali and Markhoz populations, whereas other populations (Raeini, Korki jonobe Khorasan, Lori, and Najdi) showed no signature of a genetic bottleneck in the recent past. The results showed that Iranian goats have high genetic diversity and may be of value to alternative breeding and conservation programs.
