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1.
Org Lett ; 26(14): 2702-2707, 2024 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-37094230

RESUMO

C(sp3)-rich aliphatic motifs in drug molecules are strongly associated with clinical success. Historically, the availability of compound libraries based on C(sp3)-rich cores has been limited due to the challenging direct functionalization of aliphatic rings. Instead, most small molecule drug-like libraries are diversified around central aromatic rings. Herein, we present a general approach to the synthesis of diversified libraries featuring aliphatic core rings via photoredox catalysis under mild conditions.


Assuntos
Química Farmacêutica , Catálise
2.
Angew Chem Int Ed Engl ; 58(29): 9820-9825, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31075177

RESUMO

Visible-light-induced ruthenium catalysis has enabled remote C-H alkylations with excellent levels of position control under exceedingly mild conditions at room temperature. The metallaphotocatalysis occurred under exogenous-photosensitizer-free conditions and features an ample substrate scope. The robust nature of the photo-induced mild meta-C-H functionalization is reflected by the broad functional group tolerance, and the reaction can be carried out in an operationally simple manner, setting the stage for challenging secondary and tertiary meta-C-H alkylations by ruthenaphotoredox catalysis.

3.
Angew Chem Int Ed Engl ; 55(40): 12148-9, 2016 09 26.
Artigo em Inglês | MEDLINE | ID: mdl-27490748

RESUMO

Coppercabana: The copper(I) hydride catalyzed functionalization of unactivated alkenes has been shown to be compatible with conventional carbonyl reduction. Through the combination of both pathways or complete suppression of C=O reduction in favor of C=C functionalization, methods for the stereoselective synthesis of a variety of chiral molecules have been developed.

4.
Chemistry ; 21(49): 17583-6, 2015 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-26489785

RESUMO

The B(C6 F5 )3 -catalyzed hydrogenation is applied to aldoxime triisopropylsilyl ethers and hydrazones bearing an easily removable phthaloyl protective group. The CN reduction of aldehyde-derived substrates (oxime ethers and hydrazones) is enabled by using 1,4-dioxane as the solvent known to participate as the Lewis-basic component in FLP-type heterolytic dihydrogen splitting. More basic ketone-derived hydrazones act as Lewis bases themselves in the FLP-type dihydrogen activation and are therefore successfully hydrogenated in nondonating toluene. The difference in reactivity between aldehyde- and ketone-derived substrates is also reflected in the required catalyst loading and dihydrogen pressure.

5.
Chem Soc Rev ; 44(8): 2202-20, 2015 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-25679769

RESUMO

The bond activation chemistry of B(C6F5)3 and related electron-deficient boranes is currently experiencing a renaissance due to the fascinating development of frustrated Lewis pairs (FLPs). B(C6F5)3's ability to catalytically activate Si-H bonds through η(1) coordination opened the door to several unique reduction processes. The ground-breaking finding that the same family of fully or partially fluorinated boron Lewis acids allows for the related H-H bond activation, either alone or as a component of an FLP, brought considerable momentum into the area of transition-metal-free hydrogenation and, likewise, hydrosilylation. This review comprehensively summarises synthetic methods involving borane-catalysed Si-H and H-H bond activation. Systems corresponding to an FLP-type situation are not covered. Aside from the broad manifold of C=X bond reductions and C=X/C-X defunctionalisations, dehydrogenative (oxidative) Si-H couplings are also included.

6.
Angew Chem Int Ed Engl ; 53(48): 13278-81, 2014 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-25283891

RESUMO

The hydrogenation of oximes and oxime ethers is usually hampered by N-O bond cleavage, hence affording amines rather than hydroxylamines. The boron Lewis acid B(C6F5)3 is found to catalyze the chemoselective hydrogenation of oxime ethers at elevated or even room temperature under 100 bar dihydrogen pressure. The use of the triisopropylsilyl group as a protecting group allows for facile liberation of the free hydroxylamines.

7.
PLoS One ; 8(11): e78353, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24223794

RESUMO

Helminth infections and allergy have evolutionary and clinical links. Infection with the nematode Ascaris lumbricoides induces IgE against several molecules including invertebrate pan-allergens. These antibodies influence the pathogenesis and diagnosis of allergy; therefore, studying parasitic and non-parasitic allergens is essential to understand both helminth immunity and allergy. Glutathione transferases (GSTs) from cockroach and house dust mites are clinically relevant allergens and comparative studies between them and the GST from A. lumbricoides (GSTA) are necessary to evaluate their allergenicity. We sought to analyze the allergenic potential of GSTA in connection with the IgE response to non-parasitic GSTs. IgE to purified GSTs from Ascaris (nGSTA and rGSTA), house dust mites (rDer p 8, nBlo t 8 and rBlo t 8), and cockroach (rBla g 5) was measured by ELISA in subjects from Cartagena, Colombia. Also, multidimensional proteomic approaches were used to study the extract of A. lumbricoides and investigate the existence of GST isoforms. We found that among asthmatics, the strength of IgE levels to GSTA was significantly higher than to mite and cockroach GSTs, and there was a strong positive correlation between IgE levels to these molecules. Specific IgE to GSTA was found in 13.2% of controls and 19.5% of asthmatics. In addition nGSTA induced wheal and flare in skin of sensitized asthmatics indicating that it might be of clinical relevance for some patients. Frequency and IgE levels to GSTA were higher in childhood and declined with age. At least six GST isoforms in A. lumbricoides bind human IgE. Four isoforms were the most abundant and several amino acid substitutions were found, mainly on the N-terminal domain. In conclusion, a new allergenic component of Ascaris has been discovered; it could have clinical impact in allergic patients and influence the diagnosis of mite and cockroach allergy in tropical environments.


Assuntos
Ascaris lumbricoides/imunologia , Asma/imunologia , Baratas/imunologia , Glutationa Transferase/imunologia , Proteínas de Helminto/imunologia , Imunoglobulina E/imunologia , Pyroglyphidae/imunologia , Adolescente , Adulto , Alérgenos/química , Alérgenos/genética , Alérgenos/imunologia , Sequência de Aminoácidos , Substituição de Aminoácidos , Animais , Reações Antígeno-Anticorpo , Antígenos de Dermatophagoides/química , Antígenos de Dermatophagoides/genética , Antígenos de Dermatophagoides/imunologia , Ascaris lumbricoides/química , Ascaris lumbricoides/enzimologia , Asma/genética , Baratas/química , Colômbia , Feminino , Glutationa Transferase/química , Glutationa Transferase/genética , Proteínas de Helminto/química , Proteínas de Helminto/genética , Humanos , Imunoquímica , Proteínas de Insetos/química , Proteínas de Insetos/genética , Proteínas de Insetos/imunologia , Isoenzimas/química , Isoenzimas/genética , Isoenzimas/imunologia , Masculino , Pessoa de Meia-Idade , Modelos Moleculares , Dados de Sequência Molecular , Estrutura Terciária de Proteína , Proteômica , Pyroglyphidae/química
8.
Anal Bioanal Chem ; 400(8): 2391-402, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21499951

RESUMO

The morphology of organic monolithic stationary phases based on poly(styrene-divinylbenzene) was modified by changing the ratio of monomers to microporogen in order to make them also suitable for small molecule separations. The morphology of the columns was characterized by high-resolution scanning electron micrography, showing larger primary globules and larger macropores, as well as no mesopores >20 nm in the monolithic skeleton. The permeability of the modified monoliths was approximately three times higher than that of columns which have been optimized for large molecule separations, enabling operation of a 30 cm long column at pressures below 250 bar. In the isocratic separation of dansylated amino acids, plate counts of 50000-107000 m(-1) were achievable, which are equivalent to efficiencies obtained with 3.1 µm porous particles. The separation performance for small molecules in gradient elution was investigated using mixtures of dansylated amino acids, ß-lactam antibiotics, and thyroid hormones. Finally, the modified monolithic capillary columns also proved to be highly efficient in the separation of biopolymers such as peptides and proteins, enabling peak width at half height of 3-8 s and peak capacities of 110-180 in 15-30 min gradient runs.


Assuntos
Aminoácidos/isolamento & purificação , Antibacterianos/isolamento & purificação , Poliestirenos/química , Hormônios Tireóideos/isolamento & purificação , beta-Lactamas/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Microscopia Eletrônica de Varredura , Tamanho da Partícula , Porosidade , Propriedades de Superfície
9.
Proteomics ; 10(20): 3598-609, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20859959

RESUMO

In terms of resolution, mass accuracy, and sensitivity, the Orbitrap represents one of the most potent mass analyzers available today. We here elucidate the potential of interfacing Orbitrap-MS to ion-pair RP HPLC for intact protein analysis. Using gradients of ACN and monolithic columns of 1.0 and 0.10 mm id, peak capacities between 120 and 130 were achievable within 20-25 min separation time. Compared with silica-based stationary phases, protein recovery and carryover from monolithic columns were found clearly superior. Intact proteins were detectable in a mass range covering 5.7-150 kDa with LODs in the low femtomol range. Compared with UV detection, MS detection with a scanning speed of 1.6 s per spectrum on average led to a 26% increase in chromatographic peak widths, whereas chromatographic patterns were mostly preserved in extracted ion chromatograms at an acquisition rate of 0.5 s per spectrum. Isotopic resolution of multiply charged ions was demonstrated for proteins up to 42 kDa. A micro-HPLC-Orbitrap-MS setup employing a 1.0 mm id column was utilized to characterize a 150 kDa recombinant monoclonal antibody. The applicability of nano-HPLC-Orbitrap-MS to the analysis of highly complex protein mixtures is demonstrated for the 70% ethanol extractable subproteome of wheat grains.


Assuntos
Cromatografia Líquida de Alta Pressão , Proteínas/análise , Proteômica , Espectrometria de Massas por Ionização por Electrospray , Animais , Cromatografia Líquida de Alta Pressão/instrumentação , Cromatografia Líquida de Alta Pressão/métodos , Humanos , Limite de Detecção , Peso Molecular , Proteômica/instrumentação , Proteômica/métodos , Controle de Qualidade , Espectrometria de Massas por Ionização por Electrospray/instrumentação , Espectrometria de Massas por Ionização por Electrospray/métodos
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