Demand changes meat as changing meat reshapes demand: The great meat revolution.

As consumer acceptance and overall demand for the different types of meat are important determinants of the new balance between conventional and alternative meats, our goal was to approach the changes in meat demand, as affected by the increasingly available alternative meats coupled to the challenges of conventional meat including the meat paradox, with emphasis on the Brazilian scenario. Then, some aspects of the demand for alternative meats are presented, with a brief historical background. As the decisions taken in the present shape this unprecedented revolution in the way we produce and choose whether to eat meat and, if so, which one, the details of the transition to alternative meat chains in Brazil are yet to be written. It seems even more difficult to predict which food protein items will be in higher demand in the next decades, as new products will likely present themselves for their quality as food items as well as for their ethical and environmental attributes.

Ordinal or visual analogue scales for assessing aspects of broiler chicken welfare?

When the gradation of animal welfare is scored through ordinal scales and equidistant tags are used, empirical data between tags tend to be non-equidistant. Ordinal rate scales (ORS) and visual analogue scales (VAS) were tested for the assessment of contact dermatitis on breast and abdominal areas (CD), footpad dermatitis (FP), hock burns (HB) and bird soiling (BS) in broiler chickens. Calculations regarding the inter-rater reliability, the correlation between VAS and ORS and amongst the welfare indicators measured with both scales, and the equidistance of ORS categories in relation to values measured using VAS, were made. A total of 1,303 broiler chickens from 10 flocks was assessed on-farm by three raters using both scales. Inter-rater reliabilities of CD and HB were higher when using VAS compared with ORS, but FP was lower. Correlations between scales varied between 0.90-0.97 and 0.77-0.95 (P<0.001), considering mean and individual values. Low-to-moderate correlations were observed between the four indicators using the scales. Tags on VAS that best represented ORS were non-equidistant. Results suggest both scales were reliable assessing the selected broiler chicken welfare indicators.

Twelve Threats of Precision Livestock Farming (PLF) for Animal Welfare.

Research and development of Precision Livestock Farming (PLF) is booming, partly due to hopes and claims regarding the benefits of PLF for animal welfare. These claims remain largely unproven, however, as only few PLF technologies focusing on animal welfare have been commercialized and adopted in practice. The prevailing enthusiasm and optimism about PLF innovations may be clouding the perception of possible threats that PLF may pose to farm animal welfare. Without claiming to be exhaustive, this paper lists 12 potential threats grouped into four categories: direct harm, indirect harm via the end-user, via changes to housing and management, and via ethical stagnation or degradation. PLF can directly harm the animals because of (1) technical failures, (2) harmful effects of exposure, adaptation or wearing of hardware components, (3) inaccurate predictions and decisions due to poor external validation, and (4) lack of uptake of the most meaningful indicators for animal welfare. PLF may create indirect effects on animal welfare if the farmer or stockperson (5) becomes under- or over-reliant on PLF technology, (6) spends less (quality) time with the animals, and (7) loses animal-oriented husbandry skills. PLF may also compromise the interests of the animals by creating transformations in animal farming so that the housing and management are (8) adapted to optimize PLF performance or (9) become more industrialized. Finally, PLF may affect the moral status of farm animals in society by leading to (10) increased speciesism, (11) further animal instrumentalization, and (12) increased animal consumption and harm. For the direct threats, possibilities for prevention and remedies are suggested. As the direction and magnitude of the more indirect threats are harder to predict or prevent, they are more difficult to address. In order to maximize the potential of PLF for improving animal welfare, the potential threats as well as the opportunities should be acknowledged, monitored and addressed.

Critical Perspective of Animal Production Specialists on Cell-Based Meat in Brazil: From Bottleneck to Best Scenarios.

Recently, many studies regarding consumer perception of cell-based meat have been published. However, the opinion of the professionals involved in animal production also seems relevant. In particular, veterinarians and animal scientists may be important players in the new cell-based meat production, acting as proponents or barriers to this major improvement for farm animal welfare. Therefore, our aim is to analyse the knowledge and perspective of Brazilian veterinarians and animal scientists regarding cell-based meat. Veterinarians (76.8%; 209/272) and animal scientists (23.2%; 63/272) responded to an online survey. Logistic regression, latent class and logit models were used to evaluate objective answers, and the Discourse of the Collective Subject method was used to interpret open-ended answers. Specialists who were women (62.5%; 170/272), veterinarians (76.8%; 209/272), vegetarians (7.0%; 19/272) and vegans (1.1%; 3/272) were more supportive of cell-based meat. Lack of knowledge and the connection with artificiality, the most frequent spontaneous word associated with cell-based meat by all respondents, were the main negative points highlighted. Thus, it seems fundamental to offer higher education to veterinarians and animal scientists regarding cell-based meat, since engaging them with this novel technology may mitigate both the resistance and its negative consequences for the professionals, society, the animals involved and the environment.

Animal Welfare Centres: Are They Useful for the Improvement of Animal Welfare?

Animal welfare has emerged as a scientific discipline only in the past 30 years, but a significant body of scientists has developed worldwide in this time. Over the past quarter century, several aggregations of scientists, centres of animal welfare, have become established. This can bring the benefits of the recognition of expertise and an opportunity to support animal industries in improving welfare, but it also brings the risk of scientists being influenced by these industries and failing to identify animal welfare problems as such. We conducted a bibliometric search of the scientific literature with the purpose of comparing the characteristics of publications on animal welfare that were or were not from animal welfare centres in academic institutions. We found that the number of publications on animal welfare from centres of animal welfare increased, initially, in the early 2000s and again in the last decade. Significant funding was obtained from the livestock industries for these centres. In a second search, we identified that only about 8% of scientific publications on animal welfare came from animal welfare centres, and the rest were mainly supported by funding sources other than the animal industries. It is concluded that the emergence of significant animal welfare centres, often with significant funding from industry, allows clusters of scientists to develop that could advance animal welfare knowledge more effectively than disparate scientists in isolated institutions. However, industry funding risks these scientists being aligned with industry goals that may not include animal welfare improvement to the extent required by the public. Further research to identify any ethical conflicts for scientists in animal welfare centres would be warranted.

Comparative costs of the Mouse Inoculation Test (MIT) and Virus Isolation in Cell Culture (VICC) for use in rabies diagnosis in Brazil.

The decision to use laboratory animals rather than in vitro methods is frequently based on the financial costs involved, so the objective of our study was to compare the costs of performing the Mouse Inoculation Test (MIT) and Virus Isolation in Cell Culture (VICC) for use in rabies diagnosis in Brazil. Based on observations of laboratory routines at the Pasteur Institute, São Paulo, we listed the fixed cost (FC) and variable cost (VC) items necessary to perform both tests. Considering that 200 MITs are equivalent to 350 VICC assays, in terms of facilities and staff-hours needed per month, we calculated, for both tests, the average total cost per sample, the costs of the implementation of the laboratory structure, and the costs of routine use. With regard to absolute values, the total cost was mainly influenced by FC items, as they represented 60% of the cost for the MIT and 86% of the cost for VICC. A sample analysed by the MIT costs around 205% more than one analysed by using VICC. The MIT costs 74% and 406% more than VICC, when implementation costs and routine use per month, respectively, are taken into account. Our results can assist in the resolution of costing disputes that could hinder the replacement of animals for rabies diagnosis in Brazil. The method demonstrated here might also be useful for cost comparisons in other situations where animal use still continues when validated alternatives exist.

Chronic welfare restrictions and adrenal gland morphology in broiler chickens.

Gait problems constitute an important and chronic welfare restriction for broiler chickens. The objective of the present study was to determine if adrenal gland morphology indicates chronic welfare restrictions in broiler chickens, using gait problems as the stressor. Sixty-six birds raised on a commercial unit were selected at 40 d of age and separated into groups according to gait score. One group was apparently healthy birds (AH) with gait scores of 0 to 2, and the other group had birds with gait problems (GP) that showed gait scores of 4 to 5. Birds were slaughtered and weighed, and their adrenal glands were measured and weighed; proportions of medullary and adrenocortical tissues, and lymphatic tissue and blood vessels were studied. GP birds had lower BW when compared to AH birds, and when adrenal gland weight values were adjusted to BW, a greater relative adrenal weight was observed for the GP group. Adrenals from GP birds also presented a higher proportion of blood vessels when compared to AH birds. These results might indicate increased adrenal activity and evidence of the inflammatory process as a consequence of chronic stress. Results showed that gait problems caused significant adrenal gland changes, suggesting a possible role for the study of adrenal gland morphology as an indicator of chronic welfare problems in broiler chickens.

Understanding attitudes towards the use of animals in research using an online public engagement tool.

Using an online public engagement experiment, we probed the views of 617 participants on the use of pigs as research animals (to reduce agricultural pollution or to improve organ transplant success in humans) with and without genetic modification and using different numbers of pigs. In both scenarios and across demographics, level of opposition increased when the research required the use of GM corn or GM pigs. Animal numbers had little effect. A total of 1037 comments were analyzed to understand decisions. Participants were most concerned about the impact of the research on animal welfare. Genetic modification was viewed as an intervention in nature and there was worry about unpredictable consequences. Both opponents and supporters sought assurances that concerns were addressed. Governing bodies for animal research should make efforts to document and mitigate consequences of GM and other procedures, and increase efforts to maintain a dialogue with the public around acceptability of these procedures.

Perceived barriers to the adoption of alternatives to laboratory animal use for rabies diagnosis.

The use of laboratory animals is still common practice, but some uses can be replaced by alternative methods, such as Virus Isolation in Cell Culture (VICC) instead of the Mouse Inoculation Test (MIT) for rabies diagnosis. The objective of this work was to describe current rabies diagnosis methods in Brazil and other countries, and the constraints associated with replacing this use of mice with alternative methods. Nine out of 12 Brazilian and 14 out of 43 non-Brazilian respondents reported that they currently used the MIT. Respondents in countries other than Brazil, male respondents, and those already employing in vitro methods for rabies diagnosis, expressed higher levels of support for the use of alternatives. The most frequently reported constraints associated with the use of alternatives were lack of laboratory facilities, equipment and materials (cited 17 times by respondents), and lack of financial resources (cited 15 times). The results indicate that many laboratories continue to use mice for rabies diagnosis. The proportion of laboratories that use mice appears to be especially high in Brazil, despite animal protection laws and technical guidelines that favour the use of alternatives. The barriers to the adoption of alternative methods identified in the current study provide a basis for facilitating changes in Brazil and elsewhere.

The perception of students on the use of animals in higher education at the Federal University of Paraná, Southern Brazil.

The use of animals in education and research is a controversial issue that involves ethical considerations. In Brazil, Act 11,794, which was approved in 2008, established the National Council on the Control of Animal Experimentation (CONCEA) and a database of institutions that use animals for research and education (CIUCA). This legislation also set out the regulations for the use of animals. In this study, we have evaluated the ethical issues involved in the use of animals for educational purposes at the Federal University of Paraná, through a qualitative-quantitative analysis that relied on written questionnaires. Our objective was to find out the opinions of students and staff from different academic fields, and at different stages in their professional development, on the use of animals for educational purposes. The study involved 101 students and 20 lecturers (i.e. tenure-track professors and all those who teach the students) in Biology, Pharmacology, Medicine and Veterinary Medicine. Approximately half of the students (45.5%) did not know the legislation that regulates the use of animals in education, and most of the lecturers believed that learning goals could not be achieved with alternative methods. Only 38.9% of the lecturers and 31.9% of the students trusted the usefulness of alternative methods. Furthermore, recent graduates were as unaware of the legislation, as were students in the first two years of their university courses. These results suggest that it is necessary to considerably expand the discussion on alternatives to animal use in the academic environment.

Teaching animal welfare in some veterinary schools in Latin America.

Animal welfare is an emerging topic in Latin America because of its impact on animal health, international trade, industry economic viability, and consumer perceptions. To enable veterinarians to be primary promoters of animal welfare, their training must include competencies in ethics, ethology, research, and public policy. During the past 10 years, animal welfare has gradually been incorporated into the curricula of several schools of veterinary medicine in Chile and other Latin American countries. Inclusion in these curricula is limited by a shortage of knowledgeable instructors. Research sustains inclusion of animal welfare in the curricula, but funding for research is often lacking.

Controle populacional de cães e gatos em dez Vilas Rurais do Paraná, Brasil / Dog and Cat Population Control in Ten Rural Villages, Parana, Brazil / Control poblacional de perros gatos en diez villas rurales en Paraná, Brasil

Este trabalho teve como objetivo principal o controle da população de CG em Vilas Rurais (VR) do noroeste do Paraná, através da oferta de esterilização cirúrgica e palestras educativas. Os moradores das VR trabalhadas assistiram a uma palestra sobre o controle populacional de cães e gatos e, em seguida, foram convidados a inscrever seus cães e gatos para desverminação e esterilização cirúrgica. As cirurgias foram realizadas por acadêmicos do curso de medicina veterinária supervisionados e o pós-operatório foi acompanhado pelos proprietários. Foram visitadas todas as residências das 10 VR, totalizando 330 moradias, com uma média de 1,6 animal por habitação. Do total de moradias, 67 possuíam pelo menos um animal, das quais participaram voluntariamente 66, atingindo 261 animais esterilizados. Com a elevação do índice de esterilização cirúrgica de 2,9 para 52,9, houve uma alteração na relação proprietário/animal, com aumento da valorização destes por parte de seus donos.

ABSTRACT: This work has as main objective the control of cat and dog population in ten Rural Villages (RV) of northwestern Parana, through surgical sterilization and educative lectures. Dwellers of the selected RV were offered a lecture on populationcontrol of cats and dogs, and were invited to subscribe their animals to deworming and surgical sterilization. The surgeries were performed by the supervised veterinary students and post-surgical care was followed by the owners. All homes within the 10 RV were visited, in a total of 330 households. There was an average of 1.6 pet per home, and 67% of all homes own at least one pet. The voluntary participation reached 66% of the houses which had animals and 261 animals were surgically sterilized, and castration index increased from 2.9% to 52.9%. An alteration in pet/owner relationship was achieved, with an increase in value that owners give their pets.

RESUMEN: Este trabajo tuvo como objetivo principal el control de la población de perros y gatos en 10 VR del noroeste de Paraná, a través de esterilización quirúrgica y conferencias educativas. Los habitantes de las VR trabajadas recibieron una conferencia sobre el control poblacional de perros y gatos y, enseguida, fueron invitados a inscribir sus animales para desparacitación y esterilización quirúrgica. Las cirugías fueron realizadas por estudiantes del curso de medicina veterinaria supervisados y el cuidado postoperatorio fue acompañado por los propietarios. Todas las residencias de las 10 VR fueron visitadas, totalizando 330 residencias. El promedio de animales por residencia fue de 1,6. Del total 67% tenía al menos un animal. La participación voluntaria alcanzó el 66% de las casas que tenían animales y 261 animales fueron esterilizados El índice de esterilización se incrementó de 2,9% para 52,9%. Se logró una alteración en la relación propietario/animal, con aumento de la valorización de estos por parte de sus dueños.