The influence of a packable resin composite, conventional resin composite and amalgam on molar cuspal stiffness.

Packable resin composites may offer improved properties and clinical performance over conventional resin composites or dental amalgam. This in vitro study examined the cuspal stiffness of molars restored with a packable resin composite, a conventional posterior microfilled resin composite and amalgam. Forty-eight intact caries-free human third molars were distributed into four treatment groups (n=12) so that the mean cross-sectional areas of all groups were equal. Standardized MOD cavity preparations were made and specimens restored using one of four restorative materials: (1) a spherical particle amalgam (Tytin); (2) Tytin amalgam with a dentin adhesive liner (OptiBond Solo); (3) a conventional microfilled posterior resin composite (Heliomolar); (4) a packable posterior resin composite (Prodigy Posterior). Cuspal stiffness was measured using a Bionix 200 biomaterials testing machine (MTS). Specimens were loaded vertically to 300 N at a crosshead speed of 1.0 mm/minute. Stiffness was measured at 10 intervals: (1) prior to cavity preparation (intact); (2) following cavity preparation, but before restoration; (3) seven days after restoration; then (4) 1, 2, 3, 4, 5, 6 and 12 months after restoration. All specimens were stored at 37 degrees C in deionized water throughout the study and thermocycled (5 degrees/55 degrees C; 2000 cycles) monthly for 12 months. Repeated Measures ANOVA revealed significant differences among treatment groups over time (p<0.0001). Cavity preparation reduced cuspal stiffness by more than 60%. At 12 months, the cuspal stiffness of restored teeth was, on average, 58% that of intact specimens. Neither the packable nor the conventional resin composite increased cuspal stiffness over that of amalgam.

PI3-kinase/Akt modulates vascular smooth muscle tone via cAMP signaling pathways.

Phosphatidylinositol 3-kinase (PI3-kinase) activates protein kinase B (also known as Akt), which phosphorylates and activates a cyclic nucleotide phosphodiesterase 3B. Increases in cyclic nucleotide concentrations inhibit agonist-induced contraction of vascular smooth muscle. Thus we hypothesized that the PI3-kinase/Akt pathway may regulate vascular smooth muscle tone. In unstimulated, intact bovine carotid artery smooth muscle, the basal phosphorylation of Akt was higher than that in cultured smooth muscle cells. The phosphorylation of Akt decreases in a time-dependent manner when incubated with the PI3-kinase inhibitor, LY-294002. Agonist (serotonin)-, phorbol ester (phorbol 12,13-dibutyrate; PDBu)-, and depolarization (KCl)-induced contractions of vascular smooth muscles were all inhibited in a dose-dependent fashion by LY-294002. However, LY-294002 did not inhibit serotonin- or PDBu-induced increases in myosin light chain phosphorylation or total O(2) consumption, suggesting that inhibition of contraction was not mediated by reversal or inhibition of the pathways that lead to smooth muscle activation and contraction. Treatment of vascular smooth muscle with LY-294002 increased the activity of cAMP-dependent protein kinase and increased the phosphorylation of the cAMP-dependent protein kinase substrate heat shock protein 20 (HSP20). These data suggest that activation of the PI3-kinase/Akt pathway in unstimulated smooth muscle may modulate vascular smooth muscle tone (allow agonist-induced contraction) through inhibition of the cyclic nucleotide/HSP20 pathway and suggest that cyclic nucleotide-dependent inhibition of contraction is dissociated from the myosin light chain contractile regulatory pathways.

The macromolecular associations of heat shock protein-27 in vascular smooth muscle.

BACKGROUND: Behavioral stress is a risk factor for hypertension and atherosclerosis. Stress leads to increases in the expression and phosphorylation of heat shock proteins (HSPs) in vascular smooth muscle. Two small heat shock proteins, HSP27 and HSP20, have been implicated in the regulation of the contraction and relaxation of smooth muscle. We hypothesized that stress-induced alterations in the phosphorylation of HSP27 would effect the macromolecular associations of the small HSPs. METHODS: Bovine carotid artery smooth muscle was treated with buffer alone or with the chemical stressor, arsenite. HSP27 phosphorylation was determined with isoelectric focusing immunoblotting. Macromolecular interactions were determined with subcellular fractionation, molecular sieving, and glutaraldehyde cross-linking and immunoblotting. RESULTS: Arsenite treatment led to increases in the phosphorylation of HSP27, a redistribution of some HSP27 from a cytosolic to a particulate fraction and to the formation of larger macromolecular aggregates of HSP27. Glutaraldehyde cross-linking and immunoblotting demonstrated that HSP27 existed in monomeric and dimeric forms, which suggested that the large aggregates were not simply aggregates of HSP27 but contained other proteins. CONCLUSIONS: Cellular stress leads to increases in the phosphorylation of HSP27 and to changes in the macromolecular associations of HSP27 in intact vascular smooth muscles. The functions of the small HSPs in the vascular smooth muscle may be dependent on both phosphorylation and macromolecular associations.

Physical therapy and dance in the surgical management of breast cancer. A clinical report.

This report describes a creative therapeutic exercise program that is part of a preoperative and postoperative regimen prescribed for patients with breast cancer surgery. Since August 1982, physical therapy has been offered as part of a multidisciplinary breast cancer program at our acute-care hospital. Our physical therapy department provides preoperative and postoperative evaluations and treatment for patients with breast cancer surgery. This treatment is offered on an outpatient basis and includes group dance sessions that are supervised by a licensed physical therapist and a licensed dance teacher. Physical therapy for patients with breast cancer surgery need not be limited to traditional wall-climbing exercises. Early physical therapy intervention should be directed at the unique emotional and physical problems encountered by these patients. Through dance, patients may experience the freedom of total body movement that enhances their adjustment to a new body image. An organized group setting provides a support mechanism that enables patients to share their experiences, fosters a positive attitude toward exercise, and facilitates psychological adjustment to the diagnosis of breast cancer.

Screening for lead toxicity among autobody repair workers.

Fifty-one workers in 10 small, neighborhood autobody repair shops were screened for lead toxicity using blood zinc protoporphyrin (ZPP) levels and work history questionnaires. Those with high ZPP levels (greater than 50 micrograms/dl) had further studies, including blood lead determinations. The shops were dusty with ground putty dust (lead-free) and paint spray (lead content range 0-40%). Thirteen (26%) workers had mildly elevated ZPP levels (all were full-time sander/sprayers) though few had symptoms and none had elevated blood lead levels. Eighteen controls, workers in other occupations, had normal ZPP levels. Air monitoring during inactive periods failed to reveal high lead levels (range 0-28 micrograms/m2). These data suggest that workers in autobody repair shops are at risk of lead toxicity but the exposure and biologic effects are less than in many other industries that utilize lead.