RESUMO
Retail raw meat was sampled for the presence of Shiga toxin-producing Escherichia coli (STEC) using enrichment culture and Vero cell assay. The STEC obtained were serotyped and tested for enterohaemolysin (Ehly) production and the eae gene. The presence of Shiga toxin genes (stx) was confirmed by polymerase chain reaction. A total of 18 STEC were isolated accounting for 12% of beef, 17% of lamb and 4% of pork samples. Five isolates produced Ehly but none possessed the eae gene. Five isolates were identified which possessed the stx2 gene and belonged to serotypes associated with severe infection.
Assuntos
Adesinas Bacterianas , Proteínas de Transporte , Proteínas de Escherichia coli , Escherichia coli/isolamento & purificação , Escherichia coli/patogenicidade , Carne/microbiologia , Toxinas Shiga/biossíntese , Animais , Proteínas da Membrana Bacteriana Externa/genética , Bovinos , Galinhas , Escherichia coli/classificação , Escherichia coli/genética , Genes Bacterianos , Proteínas Hemolisinas/biossíntese , Nova Zelândia , Sorotipagem , Ovinos , Toxinas Shiga/genética , Suínos , VirulênciaAssuntos
Vacinas Bacterianas/administração & dosagem , Doenças dos Bovinos/prevenção & controle , Haemophilus/imunologia , Mannheimia haemolytica/imunologia , Pneumonia/veterinária , Vírus Sincicial Respiratório Bovino/imunologia , Vacinas Virais/administração & dosagem , Animais , Anticorpos Antibacterianos/análise , Anticorpos Antivirais/análise , Vacinas Bacterianas/imunologia , Bovinos , Pneumonia/prevenção & controle , Vacinas Atenuadas/imunologia , Vacinas Virais/imunologiaRESUMO
The effects of feed restriction, early life fat restriction, and calorie:protein ratio on abdominal fat pad development and sudden death syndrome (SDS) in the broiler chicken were studied in two experiments. In Experiment 1, restricting feed intake to 90% of ad libitum intake was found to reduce significantly (P less than .01) both 49-day body weights and abdominal fat pad size while feed efficiency was significantly (P less than .01) improved compared to groups fed a wheat diet ad libitum. Corn-fed birds tended to outperform wheat fed groups. Reduced growth rate or supplementation of the diet with additional vitamins and minerals did not effect the incidence of SDS. In Experiment 2, fat restriction in the diet from 0 to 7 days initially reduced growth rate and increased feed:gain ratios. By 49 days, no effect of fat restriction on these parameters was observed. Dietary fat restriction tended to increase abdominal fat measured at 49 days of age. Lowering the calorie: protein ratio of the finishing diet significantly (P less than .05) improved feed:gain ratios measured from 29 to 49 days and tended to reduce fat pad size. Incidence of SDS was significantly (P less than .05) lower from 29 to 56 days in groups fed the 24% protein finisher diet as compared to birds fed a 19% finisher diet.
