RESUMO
Sorption of cyanocobalamin (vitamin B12) by silochrome C-120 of various graininess levels was studied under dynamic conditions. It was shown that the low-grained sorbent provided marked increasing of the cyanocobalamin sorption rate and enabled the use of the sorbent thin layers at high rates of the solution supply.
Assuntos
Dióxido de Silício/farmacologia , Vitamina B 12/farmacologia , Adsorção , Fenômenos Químicos , Físico-Química , Géis , Cinética , Sílica Gel , Soluções , Vitamina B 12/isolamento & purificaçãoRESUMO
The Ca2+ effect on the activity of cellulase [beta-1-4-endoglucanase) is quantitatively estimated. Values of partial inhibition of the enzyme activity at different Ca2+ concentrations within 2-10 mM are determined. The type of inhibition is identified from the data on kinetic analysis. The values of substrate and inhibitory constants are calculated.
Assuntos
Cálcio/farmacologia , Celulase/antagonistas & inibidores , Celulase/metabolismo , Cinética , Concentração OsmolarRESUMO
By fractionation of purine compounds (sorbtion on the cation exchange resin KU-2 (H+), extraction, precipitation of purine compounds as Ag-complexes) a "purine fraction" of the culture liquid epiphytic bacteria No. 703 isolated from barley overground organs was obtained. The presence of isopentenyl cytokinins was demonstrated by quality reactions of the purine fraction with aromatic amines and phenols as well as by the values of Rf and UV spectrum of individual compounds examined by paper chromatography of the purine fraction.