Characterization of DNA and MVA vectors expressing Nef from HIV-1 CRF12_BF revealed high immune specificity with low cross-reactivity against subtype B.

The HIV epidemic in Argentina is characterized by the high prevalence of infections caused by subtype B and BF variants. In this study, the Nef protein was used as a tool to study the impact of HIV-1 BF variants in the design of future vaccines. DNA and MVA vectors expressing Nef of the CRF12_BF recombinant form of HIV-1 were generated and characterized. After the administration of single DNAprime/MVAboost immunization schedules in Balb/c mice we found that NefBF delivered from these vectors generated a response of high specificity with low cross-reactivity against subtype B. But, when a more potent response was induced after 3 priming DNA doses and a booster with MVA virus, cross-reactivity against NefB was detected, although of lower magnitude than the NefBF specific. These results will be pivotal for vaccines designs in our region, indicating that antigens from these viral variants must be considered for a future vaccine.

[Comparative analysis of nutritional status, food intake and length of hospitalization considering two hospitals: public versus private]. / Comparación del estado nutricional, consumo alimenticio y tiempo de hospitalización de pacientes de dos hospitales, uno público y otro privado.

INTRODUCTION: To assess the nutritional status of hospitalized patients is important for the prevention of hospital malnutrition and planning of nutritional intervention. OBJECTIVE: To comparatively analyze the nutritional status of patients admitted to two hospitals, one public and another private, caring for populations with different socioeconomic profiles. CASE STUDY AND METHODS: This was a prospective study and samples were collected from patients of two hospitals, one public (PUH) and another private (PRH), which respectively represent the underprivileged and the more affluent populations. The analysis considered 51 patients from the PUH, aged between 53.9 +/- 15.6 years and 51 from the PRH, aged between 55.0 +/- 13.9 years, paired and grouped according to the following diseases: neoplasia (n+25), digestive tract disorders (n+11) and others (n+15). The statistical analysis was made by means of the T-Student test to compare PUH and PRH variables with p < 0.05, as well as to compare different variables in the same hospital, with p < 0.05; p < 0.01; p < 0.005 and p < 0.001. RESULTS: There were no statistical differences when the variables evaluated in both hospitals were compared, except for the length of hospitalization of patients with digestive tract disorders, which was longer for HPR. Upon hospitalization, 21.0% and 17.6% presented a body mass index (BMI) indicative of malnutrition. The patients with neoplasias presented current weight (CW) lower than habitual weight (HW). Both the patients with neoplasias and those with digestive tract disorders presented insufficient energy consumption in the period before hospitalization (p < 0.05). A negative correlation was found between the length of hospitalization and the current weight/habitual weight ratio (r = 0.194, 0 < 0.05, n = 102) and BMI (r=-0.186, p < 0.05, n = 102), according to Pearson's coefficient. CONCLUSION: The similarity of nutritional conditions found points to similarity of nutritional status for patients of both institutions and reflects the changes in the nutritional status of the population. The nutritional intervention should consider the nutritional needs of the population assisted.