The effect of 4 first premolar extractions on the posterior Bolton ratio.

INTRODUCTION: Overall and anterior Bolton ratios have been well covered in the orthodontic literature; however, little has been reported on posterior Bolton ratios. Considering the frequency of premolar extractions in the specialty, it would be relevant to know how the posterior occlusion is affected by premolar extractions. This study aimed to investigate how the posterior Bolton ratio is affected by the extraction of the 4 first premolars. METHODS: Fifty-five patients with Class I occlusion within 1 standard deviation of ideal anterior and overall Bolton ratios models were selected and digitized. Tooth widths were measured. Virtual extractions of 4 first premolars were performed, and a digital setup of anterior and remaining posterior teeth observing ideal occlusion relationships was executed. When space closure compromised the occlusion, preference was given to the latter. Residual interproximal spacing was digitally measured on the setups. Analysis of variance and linear regression tests were used to identify factors contributing to interproximal spacing. RESULTS: An average of 1.1 mm of net residual spacing between mandibular second premolars and first molars was observed. In 27% of the sample, at least 1.5 mm of residual space was found. In addition, 16% showed at least 2 mm of residual space. The ratio of the maxillary second premolars to the mandibular second premolars and the width of the maxillary second premolars best explain residual space (r = 0.554; r2 = 0.307). A regression equation for predicting residual space is offered. CONCLUSIONS: Ideal anterior, posterior, and overall Bolton ratios treated with extraction of 4 first premolars and ideal occlusion will likely finish with some spacing in the mandible.

Skeletal muscle dysfunction in muscle-specific LKB1 knockout mice.

Liver kinase B1 (LKB1) is a tumor-suppressing protein that is involved in the regulation of muscle metabolism and growth by phosphorylating and activating AMP-activated protein kinase (AMPK) family members. Here we report the development of a myopathic phenotype in skeletal and cardiac muscle-specific LKB1 knockout (mLKB1-KO) mice. The myopathic phenotype becomes overtly apparent at 30-50 wk of age and is characterized by decreased body weight and a proportional reduction in fast-twitch skeletal muscle weight. The ability to ambulate is compromised with an often complete loss of hindlimb function. Skeletal muscle atrophy is associated with a 50-75% reduction in mammalian target of rapamycin pathway phosphorylation, as well as lower peroxisome proliferator-activated receptor-alpha coactivator-1 content and cAMP response element binding protein phosphorylation (43 and 40% lower in mLKB1-KO mice, respectively). Maximum in situ specific force production is not affected, but fatigue is exaggerated, and relaxation kinetics are slowed in the myopathic mice. The increased fatigue is associated with a 30-78% decrease in mitochondrial protein content, a shift away from type IIA/D toward type IIB muscle fibers, and a tendency (P=0.07) for decreased capillarity in mLKB1-KO muscles. Hearts from myopathic mLKB1-KO mice exhibit grossly dilated atria, suggesting cardiac insufficiency and heart failure, which likely contributes to the phenotype. These findings indicate that LKB1 plays a critical role in the maintenance of both skeletal and cardiac function.