RESUMO
The present study was aimed to assess the effect of protein carbonylation (PC) in hepatic cells and effects of nonsteroidal anti-inflammatory drugs (NSAIDs) on indicators of tissue damage induced by liver ischemia-reperfusion injury (LIRI). Warm ischemia was performed by partial vascular occlusion during 90 min in Wistar rats. In serum, we determined the catalytic activity of Alanine Aminotransferase, Aspartate Aminotransferase, Lacticate Dehydrogenase, and Ornithine Carbamoyltransferase. In liver samples, we studied cellular alterations by means of histologic studies, lipid peroxidation, PC by immunohistochemistry, apoptosis and reactive oxygen species in bile by electron paramagnetic resonance. Based on PC data, sinusoidal endothelial cells (SEC) and Kupffer cells (KC) were the first to exhibit LIRI-associated oxidative damage and prior to parenchymal cells. Administration of piroxicam or meloxicam during the pre-ischemic period produced a highly significant decrease in all studied injury indicators. No significant differences were revealed between the protective action of the two drugs. The data shown here suggest the potential use of NSAIDs such as piroxicam or meloxicam in minimizing ischemic event-caused damage in liver. We also propose that PC may be employed as an adequate tool to assess tissue damage after oxidative stress.
Assuntos
Carbono/química , Células Endoteliais/citologia , Células de Kupffer/metabolismo , Fígado/metabolismo , Piroxicam/farmacologia , Traumatismo por Reperfusão , Tiazinas/farmacologia , Tiazóis/farmacologia , Alanina Transaminase/metabolismo , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Aspartato Aminotransferases/metabolismo , Humanos , L-Lactato Desidrogenase/metabolismo , Meloxicam , Ornitina Carbamoiltransferase/metabolismo , Estresse Oxidativo , Proteínas/metabolismo , Ratos , Ratos WistarRESUMO
INTRODUCTION: The purpose of this review is to summarize intraoperative surgical strategies available to decrease ischemia-reperfusion injury associated with liver resection and liver transplantation. MATERIAL AND METHOD: We conducted a critical review of the literature evaluating the potential applications of hepatic ischemic preconditioning (IPC) for hepatic resection surgery and liver transplantation. In addition, we provide a basic bench-to-bedside summary of the liver physiology and cell signaling mechanisms that account for the protective effects seen with hepatic IPC.
Assuntos
Hepatectomia , Precondicionamento Isquêmico , Transplante de Fígado , Animais , Fígado Gorduroso/fisiopatologia , Hepatócitos/fisiologia , Humanos , Precondicionamento Isquêmico/métodos , Fígado/fisiopatologia , Circulação Hepática , Transplante de Fígado/fisiologia , Óxido Nítrico/metabolismo , Cuidados Pré-Operatórios , Fluxo Sanguíneo Regional , Traumatismo por Reperfusão/fisiopatologia , Traumatismo por Reperfusão/prevenção & controleRESUMO
La prueba histoquímica para demostrar actividad enzimática de ATPasa en córnea humana resultó efectiva y específica para caracterizar a las células de Langerhans. Las células de Langerhans se encuentran presentes en el epitelio de la córnea humana, pero la población de las mismas difiere notablemente en las diversas regiones de este epitelio. Hay mayor cantidad en la región periférica y es nula en la región central; la diferencia estriba en la presencia y ausencia respectivamente de vascularización sanguínea y linfática en las regiones mencionadas.