Enzyme-linked imunoassays for the detection of Listeria sp. and Salmonella sp. in sausage: a comparison with conventional methods

This study was carried out comparing the conventional methods (ISO 11290-1 and BAM method, 2008) and system mini-Vidas® (Biomerieux), for detection of Listeria sp. and Salmonella sp. in cooled sausage. The immunoenzymatic method has shown to be effective for the detection of target pathogens, it has presented itself as an excellent screening method.

Pelvic differences between brachycephalic and mesaticephalic cats and indirect pelvimetry assessment.

Pelvic measurements were carried out in cats with various cranial conformations to (1) determine pelvic morphometry, (2) compare any pelvic differences with cranial conformation and gender and (3) determine whether body biometrics can be used to predict pelvic measurements. Thirteen brachycephalic and 14 mesaticephalic female cats and 17 brachycephalic and nine mesaticephalic male cats were used. Body and external pelvic measurements, as well as pelvic radiographs, were performed. Brachycephalic females all had external pelvic and radiographic measurements that were significantly smaller than those of the mesaticephalic females, including smaller pelvic inlet and outlet areas and a smaller pelvic canal shape. Brachycephalic females had wider and flatter heads than do mesaticephalic females. Similarly, brachycephalic males all have radiographic pelvic measurements that are smaller than those of mesaticephalic males. Males had larger pelvis measurements than did their female counterparts for both cranial types, and indirect pelvimetry did not demonstrate good predictive value in determining the internal pelvic measurements. Thus, we conclude that pelvic differences exist between genders and between brachycephalic and mesaticephalic cats. Furthermore, body biometric measurements do not have good predictive value for determining internal pelvic measurements.

Enzyme-linked imunoassays for the detection of Listeria sp. and Salmonella sp. in sausage: a comparison with conventional methods.

This study was carried out comparing the conventional methods (ISO 11290-1 and BAM method, 2008) and system mini-Vidas® (Biomerieux), for detection of Listeria sp. and Salmonella sp. in cooled sausage. The immunoenzymatic method has shown to be effective for the detection of target pathogens, it has presented itself as an excellent screening method.

Incidência de S aureus Meticilino-resistente (MRSA) em estetoscópios de uso hospitalar / Methicillin-resistant (MRSA) S aureus

Foram colhidas prospectivamente 150 amostras para cultura de microrganismos de estetoscópios utilizados rotineiramente no hospital por acadêmicos de Medicina, médicos e enfermeiros. Realizou-se in print direto do diagragma e swab das reentrâncias dos estetoscópios em meio de cultura ágar-sangue. Os germes foram identificados através das provas padrões. A resistência à oxacilina/meticilina foi identificada por antibiograma através do método de Kirby-Bauer e semeadura em spot. Neste estudo os estetoscópios demonstraram ser reservatórios de agentes infecciosos, demonstrando bactérias em 91,3 por cento deles. A incidência em médicos, acadêmicos e enfermeiros foi, respectivamente, 87,9 por cento, 89,3 por cento e 97,7 por cento, nos diafragmas, e 96,5 por cento, 91,3 por cento e 75 por cento, respectivamente, nas reentrâncias. Entre os estafilococos presentes, 7,3 por cento eram meticilino-sensíveis e 2 por cento eram meticilino-resistentes no diafragma, e 5,3 por cento meticilino-sensíveis e 1,3 por cento meticilino-resistentes nas reentrâncias. Os autores sugerem a desinfecção do diafragma com álcool a 70 por cento antes do atendimento de cada paciente para evitar a possível disseminação destes germes