RESUMO
The present study was designed to describe the development of germ cell neoplasia in situ in Chinchilla rabbit by administration of estradiol. The study was performed in rabbits distributed into two groups: control and 17 ß-estradiol. The determination of histological alterations and POU5F1 and c-kit proteins employed as biomarkers for the diagnosis of this neoplasia was carried out. Testicular descent and complete spermatogenesis were observed in the control group. The protein biomarkers were negative. However, in the rabbits treated with estradiol, the testes remained undescended with the gonocytes undifferentiated to spermatogonia. There were histological lesions owing to germ cell neoplasia in situ and positive to POU5F1 and c-kit proteins. These findings indicate that the chinchilla rabbit is an ideal model to study this neoplasia in which the histological characteristics and biomarkers of the disease could be clearly observed. Using this model we suggested that the persisting gonocytes could be responsible for the development of germ cell neoplasia in situ.
Assuntos
Carcinoma in Situ/patologia , Neoplasias Testiculares/patologia , Animais , Biomarcadores Tumorais/análise , Chinchila , Modelos Animais de Doenças , Imuno-Histoquímica , Masculino , Microscopia Eletrônica de Transmissão , Fator 3 de Transcrição de Octâmero/análise , Proteínas Proto-Oncogênicas c-kit/análise , Coelhos , Reação em Cadeia da Polimerase em Tempo RealRESUMO
The Chinchilla rabbit is a breed with high commercial value and nowadays is increasingly used in various fields of biomedical research, however, its postnatal reproductive biology has been little studied. The aim of the present study was to investigate the postnatal development of the testis in this rabbit breed to determine both the proliferative periods and apoptosis. 30 rabbits aged 3-100 days old were used in the study. Determination of the period of differentiation of gonocytes to spermatogonia (50dpp), the periods of proliferation and apoptosis of their cells, as well as the beginning of spermatogenesis (60dpp) and the different stages of the seminiferous epithelium cycle were made. We found that these testicular developments were closer to that of humans when compared with rats, a species commonly employed in reproductive research. On comparing these results with those obtained from other breeds, there are clear differences favoring the use of this species as a research model in the field of male reproductive biology.
