RLSuite: An Integrative R-Loop Bioinformatics Framework.

We recently described the development of a database of 810 R-loop mapping datasets and used this data to conduct a meta-analysis of R-loops. R-loops are three-stranded nucleic acid structures containing RNA:DNA hybrids and we were able to verify that 30% of expressed genes have an associated R-loop in a location conserved manner.. Moreover, intergenic R-loops map to enhancers, super enhancers and with TAD domain boundaries. This work demonstrated that R-loop mapping via high-throughput sequencing can reveal novel insight into R-loop biology, however the analysis and quality control of these data is a non-trivial task for which few bioinformatic tools exist. Herein we describe RLSuite, an integrative R-loop bioinformatics framework for pre-processing, quality control, and downstream analysis of R-loop mapping data. RLSuite enables users to compare their data to hundreds of public datasets and generate a user-friendly analysis report for sharing with non-bioinformatician colleagues. Taken together, RLSuite is a novel analysis framework that should greatly benefit the emerging R-loop bioinformatics community in a rapidly expanding aspect of epigenetic control that is still poorly understood.

EEG and skeletal development in children with different psychosocial characteristics.

Two groups of children with different socioeconomic level were studied. One minute EEG at rest was recorded in monopolar leads F3, F4, C3, C4, P3, P4, O1, O2, F7, F8, T3, T4, T5 and T6. Absolute and relative power in four EEg bands (delta, theta, alpha and beta) were computed. Radiographies of the left hand and the wrist were also obtained in all children. Age regression equations of the variables derived from EEG spectra were calculated in each group. In the group with low socioeconomic level many children had antecedents of risk factors. In this group absolute and relative power in the four bands presented a great dispersion and no correlation with age. In the group with good socioeconomic level the age regression equations of the EEG variables were significant, absolute values in the four bands decreased with age, as well as delta and theta relative power, while alpha and beta relative power increased with age. The area of the ossification center of each bone of the hand of the lower end of the ulna and radius were obtained from the X-ray film. Linear regression equations for the area of each ossification center were significant in both groups. No intercept or slope differences existed between both groups in any area. It is concluded that psychosocial disadvantage and antecedents of risk factors, although not producing any effect on skeletal development, do affect EEG maturation.