RESUMO
We have demonstrated that Amaranthus leucocarpus lectin hemagglutinating activity was powerfully inhibited by the T-antigen, containing Gal(beta 1-3)GalNAc(alpha 1-3)Ser/Thr, and the Tn-antigen, which contains GalNAc(alpha 1-3)Ser/Thr. This suggests that the acetamido group at C-2 and the axial -OH at C-4 of the N-acetyl-D-galactopyranosylamine ring are important for lectin binding. The hemagglutination assays also established that desialylated and Pronase-treated human type O erythrocytes with an M phenotype were better recognized than erythrocytes from all other blood groups. The recognition was dependent on pH and ionic strength.
Assuntos
Acetilgalactosamina/metabolismo , Eritrócitos/metabolismo , Lectinas/metabolismo , Antígenos de Grupos Sanguíneos , Metabolismo dos Carboidratos , Sequência de Carboidratos , Testes de Hemaglutinação , Humanos , Concentração de Íons de Hidrogênio , Dados de Sequência Molecular , Lectinas de Plantas , Plantas/metabolismoRESUMO
Six major neutral and acidic oligosaccharide-alditols were prepared from the jelly coat of Mexican axolotl eggs. These compounds were demonstrated to contain 3-deoxy-D-glycero-D-galacto-nonulosonic acid (dNloA) and L-fucose (Fuc). The structures of the six major oligosaccharides were established as follows: [sequence: see text]