Assuntos
Canais Iônicos/metabolismo , Técnicas de Patch-Clamp/instrumentação , Técnicas de Patch-Clamp/métodos , Células-Tronco/metabolismo , Animais , Automação , Bancos de Espécimes Biológicos , Biotecnologia/instrumentação , Biotecnologia/métodos , Linhagem Celular , Indústria Farmacêutica/instrumentação , Indústria Farmacêutica/métodos , Transferência Ressonante de Energia de Fluorescência , Humanos , Microfluídica/instrumentação , Miócitos Cardíacos/citologia , Miócitos Cardíacos/metabolismo , Neurônios/citologia , Neurônios/metabolismo , Técnicas de Patch-Clamp/economia , Robótica , Células-Tronco/citologiaAssuntos
Reação em Cadeia da Polimerase/instrumentação , Reação em Cadeia da Polimerase/métodos , Animais , DNA/análise , DNA/genética , Bases de Dados Genéticas , Humanos , Reação em Cadeia da Polimerase/tendências , Polimorfismo de Nucleotídeo Único/genética , Sensibilidade e Especificidade , Temperatura , Fatores de TempoAssuntos
Imunoensaio/métodos , Oligonucleotídeos/análise , Reação em Cadeia da Polimerase/métodos , Proteínas/análise , Proteínas/imunologia , Anticorpos/análise , Anticorpos/imunologia , DNA/análise , DNA/genética , Imunoensaio/instrumentação , Nanoestruturas/análise , Oligonucleotídeos/genética , Sensibilidade e EspecificidadeAssuntos
Biologia Computacional , Oligonucleotídeos Antissenso/análise , Oligonucleotídeos Antissenso/genética , Reação em Cadeia da Polimerase/métodos , Peso Molecular , Neovascularização Patológica/genética , Oligonucleotídeos , Oligonucleotídeos Antissenso/química , Sítios de Splice de RNA/genética , Sensibilidade e Especificidade , SoftwareRESUMO
BACKGROUND AND OBJECTIVES: There exist contradictory reports about low-intensity laser light-stimulated cell proliferation. The purpose of this study was to determine the effect of wavelength on proliferation of cultured murine cells. STUDY DESIGN/MATERIALS AND METHODS: Proliferation of primary cell cultures was measured after irradiation with varying laser wavelengths. RESULTS: Fibroblasts proliferated faster than endothelial cells in response to laser irradiation. Maximum cell proliferation occurred with 665 and 675 nm light, whereas 810 nm light was inhibitory to fibroblasts. CONCLUSIONS: These observations suggest that both wavelength and cell type influence the cell proliferation response to low-intensity laser irradiation.
Assuntos
Proliferação de Células/efeitos da radiação , Células Endoteliais/efeitos da radiação , Fibroblastos/efeitos da radiação , Terapia com Luz de Baixa Intensidade , Animais , Técnicas de Cultura de Células , Relação Dose-Resposta à Radiação , Células Endoteliais/fisiologia , Fibroblastos/fisiologia , Masculino , Camundongos , Camundongos Endogâmicos C3HRESUMO
Comparative analysis of the proteins that bind exclusively at the centromere provides evidence of an evolutionary battle that may make sense of sex.
Assuntos
Centrômero , Meiose , Animais , Centrômero/química , Proteínas Cromossômicas não Histona/genética , Evolução Molecular , Feminino , Histonas/genética , Humanos , MasculinoRESUMO
PURPOSE: Clinically bladder cells used in tissue engineering techniques will come from neuropathic bladders and not normal bladders. We determined if neuropathic bladder smooth muscle (SM) cells (SMCs) retain functional differences when cultured in vitro. MATERIALS AND METHODS: Primary cultures of SMCs were established from patients with a neuropathic bladder (5) and a normal bladder (5). Expression of alpha-SM actin and SM myosin heavy chain was determined using immunocytochemical staining and Western blot analysis. Baseline cell proliferation and the mitogenic response to angiotensin II was assessed by cell counting and cell viability assays. Cell contractility was determined for normal and neuropathic SMCs using an in vitro collagen lattice assay. Cell adherence was measured assessed using partial and complete trypsinization assays. RESULTS: Normal and neuropathic SMCs showed similar morphology in culture, and similar patterns of alpha-SM actin and SM myosin expression. Following 10 days of plating under optimal growth conditions the number of neuropathic SMCs was 170% more than normal SMCs. In response to angiotensin II neuropathic SMCs reached 54% of maximal growth capacity as opposed to 30% for normal SMCs (p <0.01). Neuropathic SMCs contracted significantly less in 10% serum and calcium ionophore (p <0.05), as determined by in vitro contractility assays. Neuropathic SMCs had 19% and 30% less adherent cells than normal SMCs (p <0.01) following isotonic solution washes and trypsinization, respectively. CONCLUSIONS: These results demonstrate that cultured neuropathic bladder SMCs possess and maintain different characteristics than normal SMCs in vitro. The potential clinical implications of using these cells in conjunction with tissue engineering techniques for the promotion of bladder regeneration requires further investigation.
