Wildfire Induces Changes in Receiving Waters: A Review With Considerations for Water Quality Management.

Wildfires have increased in frequency in many ecosystems, with implications for human health and the environment, including water quality. Increased fire frequency and urbanization also raise the prospect of fires burning into urban areas, mobilizing pollutants few have considered to date. As a result, water quality managers lack information to anticipate, respond to and potentially mitigate wildfire impacts. Here, we reviewed the scientific literature to assess wildfire effects on response endpoints of a conceptual model linking fire to water quality, quantifying response directionality, magnitude and duration. Physically, water yield, sediments, and temperature all increased post-fire. Chemically, nutrients, ions, organic chemicals, and metals increased in burned watersheds, sometimes by orders of magnitude over pre-fire or reference conditions. In select cases, post-fire concentrations exceeded aquatic life criteria or drinking water standards, at times even in the finished drinking water. Biological assemblages commonly declined after post-fire runoff events. The duration of effects was less than 5 yr for most endpoints (e.g., metals) on average following fire, although effects did extend 15 yr or more in some individual cases. We found only a few studies on pollutants mobilized from wildfire impacted urban areas with benzene contamination in drinking water and high metal concentrations in ash prominent exceptions. Overall, this review provides a resource for understanding wildfire impacts on water quality endpoints, with the goal of informing the response of managers and other decision makers to this growing problem.

Hydrodynamic analysis of intraocular pressure changes during anterior chamber procedures.

Variations in intraocular pressure (IOP), observed by us and other authors in closed-system anterior chamber procedures, are explained using a simple hydrodynamic analysis based on the Bernoulli equation. Calculations show that the sudden increases in IOP (pressure spikes) measured during anterior segment surgery are due primarily to the small size of the infusion cannulas and are exacerbated by high infusion rates. Accurate measurement of these IOP variations requires placement of the pressure sensors in situ and is not possible by remote pressure sensing at the infusion line or the console. This work indicates that closed-system procedures could benefit from accurate monitoring of intraoperative IOP and could use these measurements for automatic IOP control during surgery.

Evaluation of drug treatments for proliferative vitreoretinopathy using vitreous microtensiometry.

The effectiveness of antimetabolic and anticollagen agents against proliferative vitreoretinopathy (PVR) was assessed by vitreous microtensiometry, a new technique that measures in situ the tensile strength of vitreous membranes. Two PVR models were produced in rabbits by intravitreal injection of bovine retinal pigment epithelial (RPE) or fibroblast cells, and the animals subsequently were treated with 5-fluorouracil (5-FU) and beta-aminopropionitrile (BAPN), administered alone or in combination. The fibroblast PVR model produced high-strength membranes that did not respond significantly to these therapies. The RPE model gave lower-strength membranes that showed marginally significant decreases in strength with intravitreal 5-FU and systemic BAPN treatments. However, combination therapy showed a highly significant decrease in membrane strength and a clinically encouraging reduction in retinal detachment.

Effects of beta-aminopropionitrile after glaucoma filtration surgery: pilot human trial.

Beta-aminopropionitrile fumarate, an inhibitor of collagen cross-linking, was used topically in patients after filtration surgery for difficult cases of glaucoma (aphakic, repeat surgery, neovascular). Twenty-three patients received the drug for a total treatment period of three months following surgery. The mean preoperative intraocular pressure was 40.0 +/- 14.1 mm Hg (mean +/- standard deviation). The mean postoperative pressure was 19.0 +/- 8.9 mm Hg, with the mean follow-up at 26.4 +/- 11 weeks. The overall success rate was 74%, with success being defined as an intraocular pressure of 22 mm Hg or less at the last visit. Side effects consisted primarily of stinging immediately after the medication was applied. Inhibition of collagen cross-linking may represent an alternative method to the use of injectable 5-fluorouracil to maintain patency of filtration blebs.

The use of thiphenamil hydrochloride (Trocinate) to control wound contraction after radial keratotomy.

We studied the efficacy of a topical smooth muscle antagonist, thiphenamil hydrochloride (Trocinate), in inhibiting wound contraction in ocular tissue. Fifteen New Zealand white rabbits underwent standard eight-incision radial keratotomy (RK), and eyes were randomly assigned to treatment or control groups. As demonstrated by slit lamp corneal photographs and by ocular histology, transient inhibition of wound contraction lasted approximately one week in all treatment eyes. We conclude that thiphenamil has a temporary, but specific, effect in controlling wound contraction after ocular surgery. Smooth muscle antagonists may be useful for managing cicatricial conditions of the eye.

Variations in intraocular pressure during closed-system surgical procedures.

True intraocular pressure (IOP) during closed-chamber anterior segment surgery and vitrectomy was measured in rabbits by inserting a miniature pressure sensor directly into the anterior chamber or into the vitreous. Infusion fluid pressure was also measured simultaneously with a sensor in the infusion line. Significant and rapid changes in IOP up to 110 mm Hg were observed during routine anterior segment and vitreous procedures. None of these IOP changes affected infusion line pressure, which was governed solely by the infusion bottle height. Furthermore, raising the infusion bottle produced minimal IOP elevation whenever fluid was flowing into and out of the eye via a surgical instrument or a wound. These results show the limitations of monitoring of infusion line pressure as a means for assessing IOP and stress the need for IOP control during surgery.

Enzyme-assisted vitrectomy with bacterial collagenase. Pilot human studies.

Clostridiopeptidase A, a bacterial collagenase, was used to assist vitrectomy with membrane stripping in six patients with dense intravitreal fibroproliferative tissue associated with retinopathy of prematurity, diabetic retinopathy, or proliferative vitreoretinopathy. When it was injected intra-operatively, allowed to incubate for 15 minutes, and then removed by irrigation/aspiration, no side effects of lens opacity, lens dislocation, or retinal hemorrhage were observed. Use of this enzyme may facilitate removal of fibroproliferative tissue in certain difficult vitrectomy cases.

Effects of topical treatment with beta-aminopropionitrile after radial keratotomy in the rabbit.

This study assessed the effect of beta-aminopropionitrile treatment on the long-term curvature and compliance of corneal tissue subjected to radial keratotomy (RK). beta-Aminopropionitrile is known to inhibit cross-linking of collagen and is expected to enhance the flexibility of scar tissue, thereby reducing wound contracture and regression after RK. Seventeen adult New Zealand rabbits, weighing about 4.5 kg each, underwent RK in both eyes (eight incisions, 90% deep, 3-mm pupillary zone). Their mean preoperative corneal curvature was 44.25 diopters (+/- 0.32D at 95% confidence level). Nine of the rabbits were treated topically with beta-aminopropionitrile ointment (33 weight % in petroleum three times daily), while the control group received the petrolatum base only. The remainder of the animals received bland petrolatum gel as a control. The animals were given periodic keratometric examinations and were killed after six to eight weeks. At that time, the beta-aminopropionitrile group showed a mean reduction of 1.85 +/- 0.13 D in corneal curvature, compared with 1.18 +/- 0.08 D in the control group. The compliance and strength of the corneas were measured in vitro immediately after death. In the pressure range from 10 to 40 mm Hg, the beta-aminopropionitrile-treated corneas changed in curvature by an average of 1.4 D as compared with 0.5-D flattening for the controls. These results indicate the effectiveness of beta-aminopropionitrile treatment in enhancing longterm compliance and reducing refractive regression after RK.

Enzyme-assisted vitrectomy with bacterial collagenase. Time course and toxicity studies.

Electron microscopic analysis of intravitreal strands produced by the injection of autogenous fibroblasts showed thin, immature collagen after two weeks and the mature banded variety after four weeks. With the use of this intravitreal strand model, it was found that highly purified bacterial collagenase caused extensive digestion of scar tissue after incubation periods of 10, 15, and 30 minutes. There was no morphologic damage to cicatricial cellular elements or to the inner limiting membrane of the retina. A 45-minute exposure of retinas previously injured by photocoagulation to collagenase also did not result in morphologic evidence of damage. The use of collagenase as an adjunct to vitrectomy in cases of extreme vitreal scarring or retinal traction may decrease the complication rate of a procedure that is still extremely hazardous.

Effects of beta-aminopropionitrile after posterior penetrating injury in the rabbit.

Beta-aminopropionitrile, an inhibitor of collagen cross-linking, effectively limited the degree of posttraumatic vitreous proliferation in rabbits three weeks after double perforating injury. Light microscopic examination of the sites of perforating injury after five weeks showed minimal intravitreal fibrous proliferation in treated animals. Electron micrographs of the cicatricial vitreous collagen showed fragmentation of collagen into smaller fibrils and disintegration into amorphous areas. Inhibition of collagen cross-linking may undermine the strength of the vitreous scaffold by making vitreous and cicatricial collagen more sensitive to tissue collagenases produced during inflammation. The failure of fibrovascular membranes to grow into the vitreous may be a secondary effect of weakened vitreous support.

A dominantly inherited chorioretinal degeneration resembling sectoral retinitis pigmentosa.

A light and electron microscopic study of an eye from a 79-year-old woman diagnosed as having sector retinitis pigmentosa is presented. Prominent bone spicule pigmentation was present bilaterally in the nasal quadrant. Retinal structure in the central fundus around the fovea and extending 2-3 mm peripherally was near normal with all photoreceptors and other retinal neurons present. Extensive degeneration of the retina occurred as one proceeded toward the peripheral regions from 3-5 mm from the fovea in all quadrants. Changes were evident within the entire choroid and were most severe in regions where retinal degeneration was most pronounced. It is likely that the extensive degenerative changes present in the retina were secondary effects that follow alterations in the choroidal blood supply in individuals affected with this disorder.

Inhibition of collagen cross-linking: a new approach to ocular scarring.

A method for the production of alkali-induced conjunctival scarring in rabbits with minimal corneal involvement is presented. The lathyritic agent Beta-aminopropionitrile (BAPN), an inhibitor of collagen cross-linking, was applied topically for a period of 21 days after induction of injury. There was a statistically significant (p less than .025) increase in both the interpalpebral fissure length and width of scarred treated animals compared with the scarred control group. BAPN did not affect the interpalpebral fissure measurements of unscarred rabbit eyes. No clinical adverse effects were observed after the treatment period. Histological evaluation of the treated scar tissue was consistent with lathyrism.

Bacterial collagenase. Proposed adjunct to vitrectomy with membranectomy.

Clostridiopeptidase A digested preretinal cicatricial tissue without causing morphological alteration of normal retina during a 30-minute incubation in the rabbit. Light and transmission electron microscopy were used to determine effects on the inner limiting membrane and retinal ganglion and Müller's cells and to evaluate enzyme digestion of preretinal scarring. Removal of the injected collangenase by vitrectomy resulted in normal electroretinograms and retinal morphology 48 hours postoperatively. If the enzyme was left in the eye for 24 hours, lens opacities, partial erosion of the inner limiting membrane, and extensive hemorrhage resulted. The specificity of action of the collagenase is due to the high degree of purity of the enzyme used and a substantial biochemical difference between scar collagen and basement membrane collagen. The injection of purified collagenase capable of digesting vitreal scar tissue while leaving the retina undamaged could represent a new approach to vitrectomy, specifically to facilitate certain cases of membranectomy.

The effects of intravitreal irrigation during vitrectomy on the electroretinogram.

Intravitreal irrigation with Balanced Salt Solution Plus (BSS Plus) produced less decrease in b-wave amplitude than either normal saline solution or Balanced Salt Solution. BSS Plus was more suitable for intravitreal irrigation because it contained the appropriate bicarbonate, pH, and ionic composition necessary for maintenance of normal retinal electrical activity, and it contained glutathione, which is necessary for maintenance of endothelial cell adenosine triphosphatase and for protection against free radical damage and oxidative stress.