Do the blood anti-Müllerian hormone concentrations in young females correlate with the ovarian follicular population later in life in water buffalo?

In this longitudinal study, the anti-Müllerian hormone (AMH) levels in blood were determined in 32 Murrah buffalo females at 8, 10, 12, 16 and 19 months of age when females were synchronized and the antral follicular population (AFP) was estimated. Correlations of AFP to the AMH level at 19 months of age and retrospectively to younger ages were investigated. Then females were split into high and low AFP, and their AMH levels were compared for all ages and tested as predictors of AFP categories. The highest AMH level (p < .05) was detected at 8 months, reducing but not differing (p > .05) at 10, 12 and 16 months then reducing again (p < .05) at 19 months of age. The mean AFP was 17.6 ± 6.3 follicles, and it was positively correlated with AMH in all ages tested. High AFP females had approximately two times more antral follicles than low AFP (p < .05) and their AMH levels were higher (p < .01) than in low AFP ones in all ages. Only at 8 months, AMH levels can be used to precociously detect high AFP heifers (a cut-off point of 464.7 pg/mL; p < .05), while low AFP heifers could be detected by AMH measurements at 8, 10, 12 and 16 months of age (p < .05). We conclude that AMH of buffalo calves correlates with AFP of heifers later in life and depending on the age, its levels could be used to identify future females with low or high AFP.

Intranasal Glioma with Post-Endoscopic Resection in 1 Year Old Infant: Case Report and Literature Review / Glioma intranasal com ressecção pós-endoscópica em bebê de 1 ano: Relato de caso e revisão da literatura

Introduction Nasal gliomas - or nasal glial heterotopias - are rare congenital malformations, which correspond to 5% of the congenital nasal masses. It is a mass composed of mature glial tissue that can be located outside, inside or near the nasal region, and may or may not be connected to the brain by a fibrous pedicle. This report addresses a case of nasal glioma that suffered recurrence after endoscopic treatment. Case Report A 1-year-old boy has, since birth, a mass inside the left nostril, which obstructs and widens the bridge of the nose. Upon physical examination, it is observed that the mass does not increase in size with crying and presents negative transluminescence and Furstenberg test. Upon being biopsied, the lesion reveals malignancy and the presence of inflammatory cells. MRI ruled out communication with intracranial structures. The endoscopic resection of the heterotopia removed a mass of 3,0 2,5 1,7 cm, whose histological and immunohistochemical analysis revealed glial pattern cell proliferation in the nasal mucosa. Conclusion Considering that nasal glial heterotopy is frequently present at birth, and that newborns breathe predominantly through this route, early diagnosis of the lesion is of great importance, as it can cause signs and symptoms of respiratory distress. In addition, it is worth noting that the early approach also prevents bone deformities.

Introdução Os gliomas nasais ­ ou heterotopias gliais nasais ­ são malformações congênitas raras, que correspondem a 5% das massas nasais congênitas. Trata-se de uma massa composta por tecido glial maduro que pode se localizar no exterior, no interior ou nas proximidades da região nasal, podendo ou não estar conectado ao cérebro por um pedículo fibroso. Este relato aborda um caso de glioma nasal que sofreu recidiva após tratamento por via endoscópica. Relato do caso Um menino de 1 ano de idade apresenta, desde o nascimento, massa no interior da narina esquerda, a qual obstrui e alarga a ponte do nariz. Ao exame físico, observa-se que a massa não aumenta de tamanho com o choro e apresenta transluminescência e teste de Furstenberg negativos. Ao ser biopsiada, a lesão revela malignidade e presença de células inflamatórias. A ressonância magnética descartou comunicação com estruturas intracranianas. A ressecção endoscópica da heterotopia removeu uma massa de 3,0 2,5 1,7 cm, cujas análise histológica e imuno-histoquímica revelaram proliferação celular de padrão glial em mucosa nasal. Conclusão Considerando que a heterotopia glial nasal frequentemente se encontra presente ao nascimento, e que os recém-natos respiram predominantemente por essa via, é de grande importância o diagnóstico precoce da lesão, já que ela pode causar sinais e sintomas de desconforto respiratório. Além disso, vale destacar que a abordagem precoce também previne deformidades ósseas.

Cervical Primitive Neuroectodermal Tumor in an Adult: Case Report and Literature Review / Tumor neuroectodérmico primitivo cervical em adulto: Relato de caso e revisão da literatura

Introduction Primitive neuroectodermal tumors are rare neoplasms of the central nervous system that occur in children, with few reports in adults. These tumors are found most often in the cerebral hemispheres, with spinal cord disorders being rare. Case Report A 71-year-old man with motor and sensory deficits in the upper limbs, cervical pain, and urinary incontinence presented to the Neurosurgery Service. The physical examination revealed grade-III motor strength on the right side, grade IV- on the left upper limb, and grade IVþ on the left lower limb. A magnetic resonance imaging scan showed an expansive intramedullary lesion with a C3-C4 epicenter. Spinal decompression, lesional biopsy, and adjuvant radiotherapy were performed. The anatomopathological report showed a primitive neuroectodermal tumor. After a new treatment with adjuvant radiotherapy (20 1.8 Gy in the skull and neuroaxis and 5 1.8 Gy in tumor boost), the patient progressed without recurrence. Conclusion Since the characteristics of the tumor are similar to those of medulloblastoma, it is necessary to expand the studies on these lesions, to better understand their pathophysiology and list better diagnostic and therapeutic methods, in addition to those already available.

Introdução Os tumores neuroectodérmicos primitivos são neoplasias raras do sistema nervoso central que ocorrem em crianças, com escassos relatos em adultos. Esses tumores são encontrados mais frequentemente nos hemisférios cerebrais, sendo raros os acometimentos medulares. Relato de Caso Um homem de 71 anos com déficits motor e sensitivo em membros superiores, algia cervical e incontinência urinária apresentou-se ao Serviço de Neurocirurgia. O exame físico revelou força de grau III à direta, de grau IV- no membro superior esquerdo, e de grau IVþ no membro inferior esquerdo. Um exame de ressonância magnética denotou lesão expansiva intramedular com epicentro em C3- C4. Foram realizadas descompressão medular, biópsia lesional e radioterapia adjuvante. O laudo anatomopatológico evidenciou tumor neuroectodérmico primitivo. Após novo tratamento com radioterapia adjuvante (20 1,8 Gy no crânio e neuroeixo e 5 1,8 Gy em boost tumoral), o paciente seguiu sem recidiva. Conclusões Uma vez que as características do tumor se assemelham às do meduloblastoma, torna-se necessário ampliar os estudos acerca dessas lesões, a fim de compreender melhor sua fisiopatologia e elencar melhores métodos diagnósticos e terapêuticos, além dos já disponíveis.

Malignant Ocular Melanoma with Intradural Cervical Metastasis: Case Report and Literature Review / Melanoma ocular maligno com metástase cervical intradural: Relato de caso e revisão de literatura

Introduction The occurrence of malignant ocular melanomas is uncommon, and the association of these tumors with intradural extramedullary metastases in the cervical spine is exceptionally rare. Case Report A 62-year-old woman undergoing adjuvant chemotherapy after surgical treatment for malignant ocular melanoma begins to experience vertigo and headache. The condition evolved with walking difficulty and neck pain that was exacerbated by swallowing and mobilizing the neck. During her ocular melanoma follow-up, lesions suggestive of metastasis in the central nervous system were not evidenced until this moment. The physical examination did not show significant findings, and a cranial computed tomography scan was performed. The image showed a hyperdense lesion with postcontrast enhancement inside the vertebral canal, at the level of C1-C2. Spinal decompression and subtotal resection were performed. The anatomopathological report revealed intradural metastasis of a malignant ocular melanoma. The postoperative period was uneventful, with significant pain improvement and no recurrences. Conclusion Intradural extramedullary metastases are rare presentations of malignant ocular melanoma. In addition, less than ten similar cases have been reported in the literature. When caring for a patient with melanoma and neurological deficits, always consider evaluating central nervous system metastases. To evaluate this patient, a sensible and detailed neurological exam is extremely important to recognize the location of the deficits and guide the best approach, such as an indication for surgery.

Introdução É infrequente a ocorrência dos melanomas oculares malignos, e menos frequente ainda é a sua associação às metástases intradurais extramedulares na região cervical. Relato de Caso Uma mulher de 62 anos, submetida a quimioterapia adjuvante após tratamento cirúrgico para melanoma ocular maligno, abre um quadro de vertigem e cefaleia. O quadro evoluiu com dificuldade para deambular e dor cervical que se exacerbava ao se alimentar e mobilizar o pescoço. Durante o seguimento do melanoma ocular, não foram evidenciadas lesões sugestivas de metástase no sistema nervoso central até este momento. O exame físico não denotou alterações significativas, sendo então realizada tomografia computadorizada de crânio, cuja imagem evidenciou lesão hiperdensa com realce após contraste no interior do canal vertebral, no nível de C1-C2. Foram realizadas descompressão medular e ressecção subtotal, cujo laudo anatomopatológico revelou metástase intradural do melanoma ocular maligno. O pós-operatório seguiu sem intercorrências, com melhora significativa da dor e ausência de recidivas. Conclusão As metástases intradurais extramedulares são apresentações raras de melanoma ocular maligno. Além disso, há menos de dez casos similares relatados na literatura mundial. Ao tratar de um paciente com melanoma e déficits neurológicos, sempre considere avaliar metástases no sistema nervoso central. Para avaliar este paciente, um exame neurológico criterioso e detalhado é essencial para reconhecer a localização dos déficits e guiar o manejo adequado, como a indicação cirúrgica.

Use, perception, and local management of Copernicia prunifera (Miller) H. E. Moore in rural communities in the Brazilian Savanna.

BACKGROUND: Copernicia prunifera belongs to the Arecaceae family, and its production chain includes a set of economic activities based on the use of the stipe, petiole, fiber, fruits, roots, and leaves from which carnaúba wax is extracted, an economically valuable resource in the region. This study aimed to evaluate the uses, management, and perception of the species by local extractors. METHODS: Two communities were studied, Bem Quer, where 15 extractors of carnaúba leaves were interviewed, and Cana, where 21 extractors considered specialists were interviewed, totaling a sample of 36 interviewees. Interviewees were asked questions about uses, ways of handling, and perception of morphological variation in the carnaúba leaves. The number of leaves extracted and the income obtained from the sale of leaves were estimated from interviews and notes that each leader of extractors held during the year of the research and previous years, as well as direct observations made by researchers in the communities which recollection area of straw hold about 80 thousand individuals of C. prunifera. A regression analysis was used to explore the relationships between social variables (age, time in extractive activity, and income obtained from extraction) with the number of leaves exploited. RESULTS: The leaf was indicated as the most used part, from which an important powder is extracted for the production of wax. In addition, the leaf was also indicated to be used for fertilization and construction. The relationship between the socioeconomic variables, income from extraction, and the number of leaves extracted (in thousands) was significant (R2 = 0.73 and p < 0.001). However, the other variables analyzed in this study, such as the time spent extracting leaves and the years of residence in the community (R2 = 0.03 and p > 0.05); the number of leaves extracted and interviewee age (R2= 0.05 and p > 0.05); and the number of leaves extracted and extraction time (R2 = 0.04 and p > 0.05) did not indicate a relationship. CONCLUSION: Local extractors observed that new leaves have the highest sales value, as they have the highest production of powder. In addition, economic factor is the preponderant force that directs the management strategies of native species. For this species, however, morphological and genetic studies are needed for further clarification.

Multiple nesting attempts and long breeding seasons of Mimus gilvus (Aves: Mimidae) in southeastern Brazil

This study describes aspects of the life history of the Tropical Mockingbird Mimus gilvus (Vieillot, 1808), including the breeding period, clutch size, nestlings and a list of plants used for nesting. Nests were monitored in an area of Restinga (sand-coastal plain) habitat in a protected area in southeastern Brazil. The data from 181 nests during five breeding seasons (2010-2014) showed that the Tropical Mockingbird has a long breeding season (26.1 ± 2.6 weeks) with up to two peaks of active nests from August to March. The breeding pairs made up to four nesting attempts in the same breeding season. The mean (± SD) clutch size was 2.4 ± 0.6 eggs (n = 169). The mean (± SD) incubation period was 14 ± 0.6 days, and the nestling remained in the nest for 14.5 ± 2.2 days. The nests were built on thirty plant species, and Protium icicariba (DC.) Marchand. was the plant species most commonly used for nesting. The breeding parameters of the Tropical Mockingbird are similar to those of other Mimidae species. The knowledge gained from this study makes the Tropical Mockingbird a good choice for future studies, particularly for testing ecological and evolutionary hypotheses regarding life history attributes, habitat selection and parental investment.(AU)

Multiple nesting attempts and long breeding seasons of Mimus gilvus (Aves: Mimidae) in southeastern Brazil

This study describes aspects of the life history of the Tropical Mockingbird Mimus gilvus (Vieillot, 1808), including the breeding period, clutch size, nestlings and a list of plants used for nesting. Nests were monitored in an area of Restinga (sand-coastal plain) habitat in a protected area in southeastern Brazil. The data from 181 nests during five breeding seasons (2010-2014) showed that the Tropical Mockingbird has a long breeding season (26.1 ± 2.6 weeks) with up to two peaks of active nests from August to March. The breeding pairs made up to four nesting attempts in the same breeding season. The mean (± SD) clutch size was 2.4 ± 0.6 eggs (n = 169). The mean (± SD) incubation period was 14 ± 0.6 days, and the nestling remained in the nest for 14.5 ± 2.2 days. The nests were built on thirty plant species, and Protium icicariba (DC.) Marchand. was the plant species most commonly used for nesting. The breeding parameters of the Tropical Mockingbird are similar to those of other Mimidae species. The knowledge gained from this study makes the Tropical Mockingbird a good choice for future studies, particularly for testing ecological and evolutionary hypotheses regarding life history attributes, habitat selection and parental investment.

Diet of some passerines in south coast of Espírito Santo State, Brazil

Studies about diet of birds in Atlantic Forest are scarce. In this study, we characterized the diet of Passeriformes from the south coast of Espírito Santo State, Brazil, classifying them into trophic guilds. From 26 species a total of 94 samples were obtained by regurgitation using tartar emetic method. The 855 items found were separated into two different categories: Insects (includes other terrestrial arthropods, such as spiders and opiliones) and Fruits/Seeds. The most frequent food item was Coleopterans, and the most abundant was Hymenoptera.

Estudos sobre a dieta de aves em Mata Atlântica são raros. Neste estudo, caracterizou-se a dieta de Passeriformes na costa sul do estado do Espírito Santo, Brasil, classificando-os em guildas tróficas. Um total de 94 amostras foram obtidas de 26 espécies, utilizando o método do tártaro emético. Os 855 itens encontrados foram separados em duas categorias: Insetos (inclui outros artrópodes terrestres como aranhas e opiliões) e Frutos/Sementes. O item mais frequente foi Coleoptera e o mais abundante foi Hymenoptera.

Diet of some passerines in south coast of espírito santo state, brazil

ABSTRACT Studies about diet of birds in Atlantic Forest are scarce. In this study, we characterized the diet of Passeriformes from the south coast of Espírito Santo State, Brazil, classifying them into trophic guilds. From 26 species a total of 94 samples were obtained by regurgitation using tartar emetic method. The 855 items found were separated into two different categories: Insects (includes other terrestrial arthropods, such as spiders and opiliones) and Fruits/Seeds. The most frequent food item was Coleopterans, and the most abundant was Hymenoptera.

RESUMO Estudos sobre a dieta de aves em Mata Atlântica são raros. Neste estudo, caracterizou-se a dieta de Passeriformes na costa sul do estado do Espírito Santo, Brasil, classificando-os em guildas tróficas. Um total de 94 amostras foram obtidas de 26 espécies utilizando o método do tártaro emético. Os 855 itens encontrados foram separados em duas categorias: Insetos (inclui outros artrópodes terrestres como aranhas e opiliões) e Frutos/Sementes. O item mais frequente foi Coleoptera e o mais abundante foi Hymenoptera.

Hormônio antimülleriano pode uma realidade usada em seres humanos ser transferida a bovinos e búfalos / Antimüllerian Hormone can a reality in humans translate to bovine and buffalos

O hormônio antimülleriano (AMH) nas fêmeas é produzido especificamente pelas células da granulosa dos folículos em crescimento e controla a velocidade do crescimento folicular ao inibir a enzima aromatase. Este hormônio tem sido explorado em várias aplicações biotecnológicas, sendo a mais bem estudada a relação positiva entre a sua concentração circulante e a população folicular antral (PFA) no ovário, tornando o AMH um indicador confiável desta característica em humanos e em outras espécies. O AMH também mostrou ser um indicador prognóstico positivo do rendimento de embriões em programas de ovulação múltipla e transferência de embriões (MOET) em bovinos, porém sua utilidade para identificar fêmeas doadoras de oócitos para produção de embriões in vitro (IVEP) ainda está em discussão. Em búfalos, apenas poucos estudos foram realizados até o momento, porém devido a baixa reserva folicular ovariana na espécie e a grande variabilidade individual, que criam um desafio para ART (Assisted Reproduction Technology) na espécie, o AMH surge com grande potencial de se tornar alternativa eficiente para a identificação precoce de doadores de oócitos para IVEP. Estudos sobre AMH demonstraram claramente sua utilidade como parte do "pacote de biotecnologia reprodutiva" em algumas espécies, especialmente em seres humanos. Em animais de produção, como bovinos, e principalmente no búfalo, estudos adicionais são necessários para definir a utilidade da AMH não apenas como um bom preditor da AFP, mas também como uma ferramenta chave em programas de biotecnologia reprodutiva em todo o mundo.(AU)

The antimüllerian hormone (AMH) in females ias specifically produced by the granulosa cells of growing follicles and controls the speed of follicular growth by inhibit the enzyme aromatase. This has been recently explored in several biotechnological applications with the most well studied being the positive relationship between circulating concentration of circulating AMH and the antral follicular population (PFA) in the ovary,where AMH is becoming accepted as a reliable indicator of this characteristic in both humans and other species. AMH has also been shown to be a positive prognostic indicator of embryo yield in multiple ovulation and embryo transfer (MOET) in cattle, however its utility for identifying good oocyte donors for in vitro embryo production (IVEP) in bovines is still under debate. In buffaloes, only limited studies have been completed, however with the low ovarian follicular reserve in the species and the great individual variability creating a challenge for ART (Assisted Reproduction Technology) the enormous potential of AMH as an efficient alternative of early identification of oocyte donors for IVEP has been suggested. In summary, studies on AMH have clearly demonstrated its utility as part of the "reproductive biotechnology package" in some species, especially in humans and in farm animals such as cattle. In the buffalo, further studies are needed to define the usefulness of AMH not only as a good predictor of AFP but also as a key tool in buffalo reproductive biotechnology programs worldwide.(AU)

Diet of some passerines in south coast of Espírito Santo State, Brazil

Studies about diet of birds in Atlantic Forest are scarce. In this study, we characterized the diet of Passeriformes from the south coast of Espírito Santo State, Brazil, classifying them into trophic guilds. From 26 species a total of 94 samples were obtained by regurgitation using tartar emetic method. The 855 items found were separated into two different categories: Insects (includes other terrestrial arthropods, such as spiders and opiliones) and Fruits/Seeds. The most frequent food item was Coleopterans, and the most abundant was Hymenoptera.(AU)

Estudos sobre a dieta de aves em Mata Atlântica são raros. Neste estudo, caracterizou-se a dieta de Passeriformes na costa sul do estado do Espírito Santo, Brasil, classificando-os em guildas tróficas. Um total de 94 amostras foram obtidas de 26 espécies, utilizando o método do tártaro emético. Os 855 itens encontrados foram separados em duas categorias: Insetos (inclui outros artrópodes terrestres como aranhas e opiliões) e Frutos/Sementes. O item mais frequente foi Coleoptera e o mais abundante foi Hymenoptera.(AU)

DIET OF SOME PASSERINES IN SOUTH COAST OF ESPÍRITO SANTO STATE, BRAZIL

ABSTRACT Studies about diet of birds in Atlantic Forest are scarce. In this study, we characterized the diet of Passeriformes from the south coast of Espírito Santo State, Brazil, classifying them into trophic guilds. From 26 species a total of 94 samples were obtained by regurgitation using tartar emetic method. The 855 items found were separated into two different categories: Insects (includes other terrestrial arthropods, such as spiders and opiliones) and Fruits/Seeds. The most frequent food item was Coleopterans, and the most abundant was Hymenoptera.

RESUMO Estudos sobre a dieta de aves em Mata Atlântica são raros. Neste estudo, caracterizou-se a dieta de Passeriformes na costa sul do estado do Espírito Santo, Brasil, classificando-os em guildas tróficas. Um total de 94 amostras foram obtidas de 26 espécies utilizando o método do tártaro emético. Os 855 itens encontrados foram separados em duas categorias: Insetos (inclui outros artrópodes terrestres como aranhas e opiliões) e Frutos/Sementes. O item mais frequente foi Coleoptera e o mais abundante foi Hymenoptera.

Hormônio antimülleriano pode uma realidade usada em seres humanos ser transferida a bovinos e búfalos / Antimüllerian Hormone can a reality in humans translate to bovine and buffalos

O hormônio antimülleriano (AMH) nas fêmeas é produzido especificamente pelas células da granulosa dos folículos em crescimento e controla a velocidade do crescimento folicular ao inibir a enzima aromatase. Este hormônio tem sido explorado em várias aplicações biotecnológicas, sendo a mais bem estudada a relação positiva entre a sua concentração circulante e a população folicular antral (PFA) no ovário, tornando o AMH um indicador confiável desta característica em humanos e em outras espécies. O AMH também mostrou ser um indicador prognóstico positivo do rendimento de embriões em programas de ovulação múltipla e transferência de embriões (MOET) em bovinos, porém sua utilidade para identificar fêmeas doadoras de oócitos para produção de embriões in vitro (IVEP) ainda está em discussão. Em búfalos, apenas poucos estudos foram realizados até o momento, porém devido a baixa reserva folicular ovariana na espécie e a grande variabilidade individual, que criam um desafio para ART (Assisted Reproduction Technology) na espécie, o AMH surge com grande potencial de se tornar alternativa eficiente para a identificação precoce de doadores de oócitos para IVEP. Estudos sobre AMH demonstraram claramente sua utilidade como parte do "pacote de biotecnologia reprodutiva" em algumas espécies, especialmente em seres humanos. Em animais de produção, como bovinos, e principalmente no búfalo, estudos adicionais são necessários para definir a utilidade da AMH não apenas como um bom preditor da AFP, mas também como uma ferramenta chave em programas de biotecnologia reprodutiva em todo o mundo.

The antimüllerian hormone (AMH) in females ias specifically produced by the granulosa cells of growing follicles and controls the speed of follicular growth by inhibit the enzyme aromatase. This has been recently explored in several biotechnological applications with the most well studied being the positive relationship between circulating concentration of circulating AMH and the antral follicular population (PFA) in the ovary,where AMH is becoming accepted as a reliable indicator of this characteristic in both humans and other species. AMH has also been shown to be a positive prognostic indicator of embryo yield in multiple ovulation and embryo transfer (MOET) in cattle, however its utility for identifying good oocyte donors for in vitro embryo production (IVEP) in bovines is still under debate. In buffaloes, only limited studies have been completed, however with the low ovarian follicular reserve in the species and the great individual variability creating a challenge for ART (Assisted Reproduction Technology) the enormous potential of AMH as an efficient alternative of early identification of oocyte donors for IVEP has been suggested. In summary, studies on AMH have clearly demonstrated its utility as part of the "reproductive biotechnology package" in some species, especially in humans and in farm animals such as cattle. In the buffalo, further studies are needed to define the usefulness of AMH not only as a good predictor of AFP but also as a key tool in buffalo reproductive biotechnology programs worldwide.

Occurrence of eggs and oocysts of gastrointestinal parasites in passerine birds kept in captivity in Para State, Brazil / Ocorrência de ovos e oocistos de parasitos gastrointestinais em aves Passeriformes mantidas em cativeiro no estado do Pará, Brasil

ABSTRACT: The objective of this study was to detect helminth eggs and protozoan oocysts in samples of feces from birds of the order Passeriformes in Para State, Brazil. Fecal samples were collected individually from 403 passerine birds seized and kept in captivity in Para State. Samples were processed by the double centrifugation technique in saturated sucrose solution and the coccidial oocyst-positive samples were submitted to sporulation in potassium dichromate 2.0%. Helminth eggs and/or protozoan oocysts were observed in 43.18% (174/403) of the fecal samples examined. Coccidial oocysts were detected in 93.68% (163/174) of the positive samples, whereas helminth eggs were observed in 10.34% (18/174) of the positive samples. Oocyst sporulation occurred in 43.56% (71/163) of the samples, and only Isospora spp. oocysts were detected. Nematode eggs of the superfamilies Trichostrongyloidea (4.60%; 8/174), Ascaridoidea (0.57%; 1/174), and Trichuroidea (0.57%; 1/174) were diagnosed in the positive samples. Cestoda eggs were diagnosed in 2.87% (5/174), whereas Trematoda eggs were detected in 2.30% (4/174) of positive samples. Passerine birds seized and kept in captivity in the visited local presented parasitism by intestinal helminths and protozoan, with a predominance of infection with coccidia of the gender Isospora.

RESUMO: O objetivo do presente estudo foi detectar ovos de helmintos e oocistos de protozoários em amostras de fezes de aves da ordem Passeriformes no estado do Pará, Brasil. Amostras de fezes foram coletadas individualmente de 403 aves Passeriformes oriundas de apreensão e mantidas em cativeiro no estado do Pará. As amostras foram processadas usando a técnica de dupla centrifugação em solução saturada de sacarose e as amostras positivas para oocistos de coccídios foram submetidas à esporulação em dicromato de potássio 2,0%. Ovos de helmintos e/ou oocistos de protozoários foram observados em 43,18% (174/403) das amostras fecais examinadas. Oocistos de coccídios foram detectados em 93,68% (163/174) das amostras positivas, enquanto que ovos de helmintos foram observados em 10,34% (18/174). A esporulação de oocistos ocorreu em 43,56% (71/163) das amostras, e somente oocistos de Isospora spp. foram detectados. Ovos de nematódeos das Superfamílias Trichostrongyloidea (4,60%; 8/174), Ascaridoidea (0,57%; 1/174) e Trichuroidea (0,57%; 1/174) foram diagnosticados nas amostras positivas. Ovos de Cestoda foram diagnosticados em 2,87% (5/174), enquanto que ovos de Trematoda foram detectados em 2,30% (4/174) das amostras positivas. Aves Passeriformes oriundas de apreensão e mantidas em cativeiro nas áreas visitadas estavam parasitadas por helmintos e protozoários, predominando a infecção por coccídios do gênero Isospora.

Avaliação da viabilidade do sêmen bubalino congelado pelos métodos convencional e automatizado (resultados parciais) / Evaluation of viability the buffalo semen frozen by conventional and automated methods (partial results)

In the Artificial Insemination it is essential to the use of frozen semen, which causes damage to thestructure of sperm. To avoid these cellular damage, there is a need to assess the viability of frozen semen buffaloby conventional and automated methods, and to predict which of the methods retrieve highest number of viablecells post-thawing. The aim of this study was to evaluate the efficiency of these two methods in buffalo semenfreezing. Semen was obtained from buffalo breeding and diluted in TES-TRIS. After semen freezing, the sampleswere evaluated for motility and vigor. There was no difference between the automated and conventionalmethods, respectively, for motility (67,5%±10 and 69,37±9,28), and the vigor (3,06±0,57 and 3,06±0,68).Therefore, it is concluded that the freezing methods are effective in cryopreservation the semen buffalo, however,it is suggested that more specific tests are performed to validate the protocols. (AU)

Fracionamento de doses de sêmen bovino criopreservado para utilização na fertilização in vitro de embriões / Fractionation of bovine semen doses cryopreserved for use in vitro fertilization

The use of frozen semen for PIVE is not fully utilized, as usually using a full dose of semen being wasteda large number of sperm that could be used in other PIVE, for these reasons, the objective of this work was toevaluate the fractionation of 0.25 ml straws in frozen bovine semen doses, divided into four equal sections forlater use in IVF technique in order to avoid wasting sperm present in the straw. Motility, vigor and spermconcentration analyzes were performed. There was observed no effect of vane fractionation process on spermparameters evaluated, such as motility 58,75%±7,6, vigor 2,75±1,6, and spermatic concentration average(3,7x106) between the sectioned parts. Thus, it is concluded that the semen dose fractionation method ofcryopreserved bull into four sections is viable for in vitro fertilization techniques, provided that there is nocompromise on the number and viability of sperm cells.(AU)