RESUMO
Glioblastoma multiforme (GBM) is a highly malignant brain tumor. Its standard treatment includes a combination of surgery, radiation, and chemotherapy. The last involves the oral delivery of free drug molecules to GBM such as Temozolomide (TMZ). However, this treatment has limited effectiveness owing to the drugs premature degradation, lack of cell selectivity, and poor control of pharmacokinetics. In this work, the development of a nanocarrier based on hollow titanium dioxide (HT) nanospheres functionalized with folic acid (HT-FA) for the targeted delivery of temozolomide (HT-TMZ-FA) is reported. This approach has the potential benefits of prolonging TMZ degradation, targeting GBM cells, and increasing TMZ circulation time. The HT surface properties were studied, and the nanocarrier surface was functionalized with folic acid as a potential targeting agent against GBM. The loading capacity, protection from degradation, and drug retention time were investigated. Cell viability was performed to assess the cytotoxicity of HT against LN18, U87, U251, and M059K GBM cell lines. The cell internalization of HT configurations (HT, HT-FA, HT-TMZ-FA) was evaluated to study targeting capabilities against GBM cancer. Results show that HT nanocarriers have a high loading capacity, retain and protect TMZ for at least 48 h. Folic acid-functionalized HT nanocarriers successfully delivered and internalized TMZ to glioblastoma cancer cells with high cytotoxicity through autophagic and apoptotic cellular mechanisms. Thus, HT-FA nanocarriers could be a promising targeted delivery platform for chemotherapeutic drugs for the treatment of GBM cancer.
Assuntos
Glioblastoma , Nanosferas , Humanos , Temozolomida/farmacologia , Temozolomida/uso terapêutico , Glioblastoma/tratamento farmacológico , Glioblastoma/patologia , Ácido Fólico , Linhagem Celular TumoralRESUMO
Traditionally, rule interactions are handled at implementation time through rule task properties that control the order in which rules are executed. By doing so, knowledge about the behavior and interactions of decision rules is not captured at modeling time. We argue that this is important knowledge that should be integrated in the modeling phase. In this project, we build upon current work on a conceptual schema to represent clinical knowledge for decision support in the form of if
Assuntos
Algoritmos , Sistemas de Apoio a Decisões Clínicas , Técnicas de Apoio para a Decisão , Registros Eletrônicos de Saúde , Internet , Processamento de Linguagem Natural , Vocabulário Controlado , SemânticaRESUMO
Controlling reaction temperature for a set time enables the size of gold nanoparticles autoreduced on the surface of polyaniline nanofibers to be controlled. The size of the gold nanoparticles can be used to tune the electrical bistable memory effect in gold/polyaniline nanofiber composite devices. Turn-on voltages and on/off ratios improve with decreasing nanoparticle size, making this a promising method to enhance performance and create smaller devices. Long-term stability of the composites can be improved by the addition of stabilizers following autoreduction of the gold nanoparticles.
Assuntos
Compostos de Anilina/química , Cristalização/métodos , Ouro/química , Nanoestruturas/química , Nanotecnologia/instrumentação , Processamento de Sinais Assistido por Computador/instrumentação , Condutividade Elétrica , Desenho de Equipamento , Análise de Falha de Equipamento , Nanoestruturas/ultraestrutura , Tamanho da PartículaRESUMO
We have developed a substrate-based bottom-up approach to assemble two different color emitting quantum dots (CdSe/ZnS core/shell QDs) on the surface of a novel virus mutant, CPMV-T184C. Electrical characteristics of individual hybrids were investigated by conductive atomic force microscopy for potential digital memory applications (i.e., RAM). These individual 40 nm CPMV-QD(1,2) hybrids exhibited reversible bistable electrical behavior during repeatable writing-reading-erasing processes at the nanoscale.