RESUMO
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Human leukocyte antigen (HLA)-G is an immunomodulatory molecule discovered for the first time in the maternalfetal interface. In cancer context, where high number of natural killer (NK) cells is described, the presence of HLA-G in its soluble form is thought to be essential for NK cellssignaling. To evaluate intracellular signaling in NK cells upon HLA-G soluble forms stimulation, we investigate the role of soluble HLA-G (HLA-G5- and HLA-G1 shedding form) stimulation on classical nuclear factor (NF)κB pathway activation. We reported that these two forms of soluble HLA-G could activate NFκB in NK cells. NFκB activation in NK cells does implicate neither phosphatidylinositol 3-kinase (PI3K) nor MEK (MAP kinase kinase) as demonstrated after specific inhibition experiments. We demonstrated elsewhere that NFκB activation in NK cells is not implicated in cytotoxicity inhibition by HLA-G. Our findings may suggest the important role played by NFκB activation after soluble HLA-G stimulation in other NK cells function (AU)