RESUMO
Primary undifferentiated sarcoma of the liver is a rare tumor, being documented primarily in the pediatric age group. This report describes the occurrence of such a tumor in a 55-year-old white woman with Meyenburg's complexes of the liver and the CRST syndrome. The clinicopathologic features of the tumor in the adult are characterized and the literature is reviewed.
Assuntos
Neoplasias dos Ductos Biliares/patologia , Hamartoma/patologia , Neoplasias Hepáticas/patologia , Neoplasias Primárias Múltiplas/patologia , Sarcoma/patologia , Neoplasias dos Ductos Biliares/diagnóstico , Neoplasias dos Ductos Biliares/ultraestrutura , Ductos Biliares Intra-Hepáticos , Citoesqueleto/ultraestrutura , Feminino , Hamartoma/diagnóstico , Hamartoma/ultraestrutura , Humanos , Neoplasias Hepáticas/diagnóstico , Neoplasias Hepáticas/ultraestrutura , Pessoa de Meia-Idade , Neoplasias Primárias Múltiplas/diagnóstico , Neoplasias Primárias Múltiplas/ultraestrutura , Sarcoma/diagnóstico , Sarcoma/ultraestrutura , Escleroderma Sistêmico/complicaçõesRESUMO
We find lag phases exceeding 20 min in measuring creatine kinase activity, by using the kinetic creatin phosphate leads to creatine assay, in sera of some patients with carcinoma metastatic to the liver. Such long lag phases are accompanied by a decreased apparent enzyme activity. These problems are eliminated by adding sulfhydryl agents to the serum before assay, but not by adding more of such agents to the assay reagent. beta-Mercaptoethanol is supperior to Cleland's reagents, glutathione, and cysteine. The long lag phases could not be explained by inadequate activity of the coupling enzymes, interference with the coupling steps, high proportions of cardiacisoenzymes activity, simple oxidation of the enzyme, low concentrations of albumin, or increased concentrations of glutathione reductase, lactate dehydrogenase, or uric acid. We conclude that the prolonged lag phases reflect inadequate reactivation of the enzyme by sulfhydryl agents under the usual assay conditions. Reactivation before assay can prevent potentially serious negative errors in the assay of creatine kinase.