RESUMO
BACKGROUND: Mexico has the largest number of clinical cases of actinomycetoma in North and South America. Species originally identified by less specific methods have been recently reclassified as other known species or as new species. OBJECTIVE: To assess, by 16S rRNA gene sequencing and phenotypic methods, the species distribution of 18 human clinical isolates originally identified as N. brasiliensis, some of them isolated between 1947 and 1959 in Mexico City. METHODS: Clinical isolates came from the Hospital General, "Dr. Manuel Gea Gonzalez", and Instituto Nacional de Diagnóstico y Referencia Epidemiológica (INDRE) in Mexico, D.F. The strains used in this study included 15 clinical strains isolated between 1947 and 1959 that were originally identified as N. brasiliensis and three more strains obtained in 2007 identified as Nocardia spp. The isolates were identified genotypically by sequencing the 16S rRNA gene, and their phenotypic profiles were obtained with the API Coryne(®) system. Antibiotic susceptibility patterns were tested according to the protocol of the Comité de l'antibiogramme de la Société française de microbiologie[4]. RESULTS: According to 16S rRNA gene, sequencing were identified among 18 human clinical isolates as Nocardia farcinica (n=11) and Nocardia brasiliensis (n=7). A high number of the strains were susceptible to the majority of the antibiotics tested. The phenotypic profiles of the strains were quite uniform for N. farcinica and some variability was observed for N. brasiliensis strains. CONCLUSION: N. farcinica was the most prevalent species identified. Modern methodologies should be applied in clinical laboratories to accurately identify etiological agents.
Assuntos
Nocardiose/microbiologia , Nocardia/isolamento & purificação , Antibacterianos/farmacologia , Técnicas de Tipagem Bacteriana , Genótipo , Humanos , México/epidemiologia , Testes de Sensibilidade Microbiana , Nocardia/classificação , Nocardia/efeitos dos fármacos , Nocardia/genética , Nocardiose/epidemiologia , Fenótipo , Filogenia , RNA Ribossômico 16S/genéticaRESUMO
Steroidal glycoalkaloids of many Solanum species have recognized biological activities, especially those containing the glycosyl moiety alpha-L-rhamnopyranosyl-(1-->2)-[alpha-L-rhamnopyranosyl-(1-->4)]-D-glucopyranose (chacotriose) whose peracetate is here synthesized and characterized by complete 1H and 13C NMR assignment.
Assuntos
Ácido Peracético/síntese química , Solanina/análogos & derivados , Tropanos/síntese química , Configuração de Carboidratos , Sequência de Carboidratos , Dados de Sequência Molecular , Extratos Vegetais/síntese química , Alcaloides de Solanáceas/química , Solanina/química , Solanum tuberosum/químicaRESUMO
Con el objetivo de conocer menejo, evolucion y frecuencia de los esquemas de tratamientos en pacientes con priapismo previamente diagnosticado en el Hospital Francisco Moscoso Puello revisamos 14 casos durante el 1983-1988; encontrandonos que el 78 por ciento asistio al hospital antes de las 72 horas de iniciado el cuadro, usandose tratamiento medico al 92.8 por ciento y persistiendo el 100 por ciento . Se aplico tratamiento quirurgico al 92.8 por ciento (tecnica Chester-Winter) presentado resolucion del cuadro 53.8 por ciento y cursando con persistencia 46.2 por ciento y de estos 23 por ciento sufrio impotencia sexual. Se concluye senalando que el tratamiento medico usado es inefectivo por lo que recomendamos ensayar medidas alternativas como el uso de neostigmina, ketamina y diazepan
Assuntos
Humanos , Masculino , Priapismo/cirurgia , Priapismo/tratamento farmacológico , Estudos RetrospectivosRESUMO
MONOGRAFIA presentada a la Escuela de Posgrado Asociacion Odontologica Argentina
Assuntos
OdontopediatriaRESUMO
MONOGRAFIA presentada a la Escuela de Posgrado Asociacion Odontologica Argentina
Assuntos
Odontopediatria , OdontopediatriaRESUMO
MONOGRAFIA presentada a la Escuela de Posgrado Asociacion Odontologica Argentina