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1.
Int J Infect Dis ; 62: 56-58, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28751008

RESUMO

OBJECTIVES: This study sought to determine the genotypes of circulating Bordetella pertussis, the causative agent of pertussis, in Cambodia by direct molecular typing of clinical specimens. METHODS: DNA extracts from nasopharyngeal swabs obtained from 82 pertussis patients in 2008-2016 were analyzed by multilocus variable-number tandem repeat analysis (MLVA). B. pertussis virulence-associated allelic genes (ptxA, prn, and fim3) and the pertussis toxin promoter ptxP were also investigated by DNA sequence-based typing. RESULTS: Forty-four DNA extracts (54%) yielded a complete MLVA profile, and these were sorted into 8 MLVA types (MT18, MT26, MT27, MT29, MT43, MT72, MT95, and MT200). MT27 and MT29, which are common in developed countries, were the predominant strain types (total 73%). The predominant profile of virulence-associated allelic genes was the combination of ptxP3/ptxA1/prn2/fim3A (48%). MT27 strains were detected during the entire study period, whereas MT29 strains were only found in 2014-2016. CONCLUSIONS: The B. pertussis population in Cambodia, where a whole-cell pertussis vaccine (WCV) has been continuously used, resembled those observed previously in developed countries where acellular pertussis vaccines are used. Circulating B. pertussis strains in Cambodia were distinct from those in other countries using WCVs.


Assuntos
Bordetella pertussis/genética , Coqueluche/microbiologia , Alelos , Bordetella pertussis/patogenicidade , Camboja/epidemiologia , DNA Bacteriano , Genótipo , Humanos , Repetições Minissatélites , Epidemiologia Molecular , Tipagem Molecular , Toxina Pertussis/genética , Vacina contra Coqueluche/imunologia , Regiões Promotoras Genéticas , Análise de Sequência de DNA , Coqueluche/epidemiologia
2.
PLoS One ; 12(7): e0181181, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28700751

RESUMO

In 2013, national serosurveillance detected a high seroprevalence of antibodies to pertussis toxin (PT) from Bordetella pertussis among Japanese adults. Thus, we aimed to determine the cause(s) of this high seroprevalence, and analyzed the titers of antibodies to PT and filamentous hemagglutinin (FHA) among adults (35-44 years old), young children (4-7 years old), and older children (10-14 years old). Our quantitative analyses revealed that adults had higher seroprevalences of anti-PT IgG and PT-neutralizing antibodies, and similar titers of anti-FHA IgG, compared to the young and older children. Positive correlations were observed between the titers of PT-neutralizing antibodies and anti-PT IgG in all age groups (rs values of 0.326-0.522), although the correlation tended to decrease with age. The ratio of PT-neutralizing antibodies to anti-PT IgG was significantly different when we compared the serum and purified IgG fractions among adults (p = 0.016), although this result was not observed among young and older children. Thus, it appears that some adults had non-IgG immunoglobulins to PT. Our analyses also revealed that adults had high-avidity anti-PT IgG (avidity index: 63.5%, similar results were observed among the children); however, the adults had lower-avidity anti-FHA IgG (37.9%, p < 0.05). It is possible that low-avidity anti-FHA IgG is related to infection with other respiratory pathogens (e.g., Bordetella parapertussis, Haemophilus influenzae, or Mycoplasma pneumoniae), which produces antibodies to FHA-like proteins. Our observations suggest that these adults had been infected with B. pertussis and other pathogen(s) during their adulthood.


Assuntos
Adesinas Bacterianas/imunologia , Anticorpos Antibacterianos/imunologia , Toxina Pertussis/imunologia , Adolescente , Adulto , Bordetella parapertussis/imunologia , Criança , Pré-Escolar , Feminino , Haemophilus influenzae/imunologia , Humanos , Imunoglobulinas/imunologia , Masculino , Mycoplasma pneumoniae/imunologia , Estudos Soroepidemiológicos
3.
J Microbiol Methods ; 133: 20-22, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-27974227

RESUMO

We evaluated a commercial loop-mediated isothermal amplification (LAMP) assay kit for Bordetella pertussis detection. The LAMP primers were designed to target the ptxP1 allele of the pertussis toxin promoter, but the assay could detect B. pertussis ptxP3 and ptxP8 strains in addition to ptxP1 strains, with high analytical sensitivity.


Assuntos
Infecções por Bordetella/diagnóstico , Bordetella pertussis/isolamento & purificação , DNA Bacteriano/isolamento & purificação , Técnicas de Amplificação de Ácido Nucleico , Alelos , Infecções por Bordetella/microbiologia , Bordetella pertussis/genética , Primers do DNA , Genes Bacterianos , Toxina Pertussis/análise , Polimorfismo de Nucleotídeo Único , Regiões Promotoras Genéticas , Kit de Reagentes para Diagnóstico , Sensibilidade e Especificidade
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