Synthesis of an IRMOF-1@SiO2 Core-Shell and Amino-Functionalization with APTES for the Adsorption of Urea and Creatinine Using a Fixed-Bed Column Study.

Kidney dysfunction is a clinical disease that disables the kidneys to remove the waste products and uremic toxins from the circulation and may lead to fatal kidney failure. Hemodialysis is advantageous in this circumstance since it prevents the accumulation of waste products in the body and facilitates the removal of uremic toxins. However, hemodialysis cannot entirely remove some uremic toxins, such as urea and creatinine. In this paper, a high-performance fixed-bed column for urea and creatinine removal was offered. As a result, a MOF layer was built on SiO2, which was then amino-functionalized using APTES. Numerous assays were used to characterize the final adsorbent. The adsorption of urea and creatinine was evaluated in batch and continuous conditions. Thus, it was demonstrated that the adsorption behavior of A(0.2)-IRMOF-1@SiO2 followed the Langmuir isotherm, and it exhibited the maximum adsorption capacity. The batch experiment determined that urea and creatinine had an adsorption capacity of 1325.73 and 625.00 mg·g-1, respectively. The adsorption capacity was increased, which was due to the presence of amino groups (APTES) on the MOF surface. The continuous operation was evaluated using the A(0.2)-IRMOF-1@SiO2 fixed-bed column. Thomas and Nelson's models were examined to achieve a better understanding of the adsorption behaviors. The A(0.2)-IRMOF-1@SiO2 fixed-bed column successfully removed 92.57% of urea and 80.47% of creatinine. The separation factor for urea in comparison to creatinine was 2.40 in the A(0.2)-IRMOF-1@SiO2 fixed-bed column.

Synthesis of NMOF-5 Using Microwave and Coating with Chitosan: A Smart Biocompatible pH-Responsive Nanocarrier for 6-Mercaptopurine Release on MCF-7 Cell Lines.

Cancer is one of the most difficult diseases to treat, threatening the lives of millions of people today. So far, various methods have been used to treat cancer, each having its drawbacks. One of these methods is treatment with anticancer drugs, which unfortunately have severe side effects. One of the causes of these complications is the nonspecific effects of anticancer drugs, which attack normal cells in addition to cancer cells and damage healthy tissues. In this study, we are trying to reduce the side effects and increase the efficacy of the drug by providing smart drug delivery. The metal-organic framework (MOF) was rapidly synthesized using a microwave method and at the nanoscale. The particle size of NMOF-5 was 18-20 nm, and its surface area was 2690 m2·g-1. A chitosan polymer coating was formed on the nanocarrier after 6-mercaptopurine was introduced. The biocompatible nanocarrier exhibited a high capacity to adsorb the drug. The biocompatible nanocarrier slowly and uniformly released 96.78% of the drug in a simulated solution at pH 5 and 20.52% at pH 7.4. This showed that CS-6-MP-NMOF-5 released the drug smartly and pH-sensitively. The stability of the biocompatible nanocarrier was studied at different pH values and remained stable at pH 5 for up to 48 h. The toxicity study of the MCF-7 cell line at different concentrations for 24 h showed the excellent performance of the biocompatible nanocarrier compared to the free drug in terms of toxicity to breast cancer cells.

Sensitive detection of tamsulosin hydrochloride based on dual-emission ratiometric fluorescence probe consisting of amine-carbon quantum dots and rhodamine B.

In this work, amine-carbon quantum dots (CQDs)/rhodamine B (RhB) ratiometric fluorescent (RF) sensor was employed for effective and selective determination of tamsulosin hydrochloride (TMS) based on a dual-emission fluorescence system. Although the function of amine-CQDs is to transfer the specific interaction between TMS and sensor into detectable fluorescence (FL) signals, RhB as a reference unit has been employed to omit internal and external effects. The FL signal was quenched by adding the TMS at 442 nm; nevertheless, it did not change at 569 nm. The material characterization and investigation of the sensing mechanism were done. The optimization of pH, the volumetric ratio of CQDs to RhB, and interaction time parameters were carried out by the one-variable-at-a-time (OVAT) method. The quantitative analysis of the concentration of TMS for this RF sensor in a linear range of 0.446-7.083 µg mL-1 (1.091-17.338 µM) was obtained (R2 = 0.9969, n = 3) under optimum conditions. The limit of detection and quantitation values were estimated to be 0.033 µg mL-1 (0.081 µM) and 0.109 µg mL-1 (0.267 µM), respectively. The repeatability of intra-day and inter-day were less than one percent. This inexpensive RF probe was well applied to determine TMS in biological fluids, and acceptable achievements were obtained.

Evaluation of spirometry findings with severity of coronary artery disease in smoker patients undergoing angiography in military hospital during 2019-2020.

BACKGROUND: Coronary heart disease is the most common cardiovascular worldwide, and some factors can affect the prognosis of this disease. So, in this study, we aimed to examine the relationship between spirometry and cardiovascular risk factors in patients undergoing coronary angiography who were referred to military hospitals. METHODS: In this cross-sectional study, 200 smokers referred to military hospital for angiography, were enrolled in terms of the inclusion and exclusion criteria between 2019 and 2020. The severity of the coronary artery involvement was determined using Gensini score. The relationship among spirometry and the forced expiratory volume in one second (FEV1), forced vital capacity (FVC), and FEV1/FVC with other variables including lipid profile, demographic findings, blood pressure, physical activity, and severity of coronary artery involvement were also exanimated. RESULTS: The frequency of severity of coronary involvement were reported as 3.5% with 25% involvement, 7% with 26-50% involvement, 5.5% with 51-75% involvement, 27.5% with 76-90% involvement, 47% with 91-99% involvement, and 9.5% with 100% involvement. In addition, there was no significant relationship between severity of coronary involvement and FEV1 and FVC (P>0.05). However, there was a significant difference between the groups based on FEV1/FVC (P=0.003), in which the mean of FEV1/FVC was significantly lower in higher severity of coronary involvement compared to lower severity of coronary involvement. There were significant relationships between severity of coronary involvement and body mass index, fasting blood sugar, high-density lipoprotein and low-density lipoprotein, cholesterol, triglyceride, waist circumference, systolic blood pressure, diastolic blood pressure, physical activity, and smoking (P<0.05). CONCLUSION: There is an association between pulmonary diseases and coronary disease, in which the increased coronary involvement severity is associated with the decreased FEV1/FVC.

A novel hybrid fluorescence probe sensor based on metal-organic framework@carbon quantum dots for the highly selective detection of 6-mercaptopurine.

In the present study, MIL-101(Fe) and amine-carbon quantum dots (CQDs) were combined via a post-synthetic modification (PSM) method; thus, a novel MIL-101(Fe)@amine-CQD hybrid fluorescent probe sensor for the detection of 6-mercaptopurine (6-MP) was synthesized. Amine-CQDs as a fluorescent material can convert the bonding interaction between MIL-101(Fe) and 6-MP into recognizable fluorescence signals, and MIL-101 (Fe) as an adsorbent can pre-concentrate 6-MP. Hereupon, this new sensor demonstrates high selectivity and sensitivity towards the detection of 6-MP. The addition of 6-MP to this probe quenches the fluorescence signal at 599 nm. In this study, factors such as pH, response time, and concentration of MIL-101(Fe)@amine-CQDs were optimized by the one-factor-at-a-time (OFAT) method. Under optimal conditions, the relationship between the fluorescence enhancement factor and the concentration of 6-MP for this sensor in the range of 0.1667-1.0000 µg L-1 was linear (R2 = 0.9977, n = 3). The limit of detection and limit of quantitation were 55.70 ng L-1 and 202.06 ng L-1, respectively, which are better than similar techniques. The repeatability of intra-day and inter-day was 2.4% and 4.7%, respectively. This fluorescent sensor was employed to determine 6-MP in real samples and exhibited acceptable results.