RESUMO
This cross-sectional study investigated California dentists' knowledge and opinion of the interface between oral and overall health. A questionnaire and cover letter explaining the purpose of the study were mailed to 1,100 randomly selected California licensed general dentists. The survey measured dentists' knowledge and opinions of the interface between oral and overall health as well as their recommendations for strengthening this interface. Dentists believed that there was minimal interface between oral and overall health care.
Assuntos
Conhecimentos, Atitudes e Prática em Saúde , Nível de Saúde , Saúde Bucal , Padrões de Prática Odontológica/estatística & dados numéricos , California , Estudos Transversais , Humanos , Pessoa de Meia-Idade , Inquéritos e QuestionáriosRESUMO
We have investigated protein kinase C (PKC) regulation by 1,25-(OH)2D3 in the rat osteosarcoma cell line ROS 17/2.8 since previous reports have implicated PKC in the 1,25-(OH)2D3-mediated regulation of osteocalcin gene expression (J. Biol. Chem. 267 (1992) 12562; Endocrinology 136 (1995) 5685). Here we report that 1,25-(OH)2D3 increased PKCalpha, but not PKCbetaI, epsilon or zeta, levels in the nuclear fraction in a time-dependent manner. Unlike PMA, 1,25-(OH)2D3 did not alter the association of any of the expressed PKC isoenzymes with the plasma membrane. Treatment with 20 nM 1,25-(OH)2D3 for 15 min, 30 min, 1 h and 24 h increased PKCalpha levels in the nuclear fraction by 2.3- to 2.6-fold. Nuclear PKCalpha expression was also increased with doses of 1,25-(OH)2D3 as low as a 0.05 nM. 1,25-(OH)2D3-mediated stabilization of osteocalcin mRNA (Arch. Biochem. Biophys. 332 (1996) 142) was inhibited with bisindolylmaleimide treatment, suggesting that PKCalpha may be involved in the 1,25-(OH)2D3-mediated regulation of osteocalcin gene expression.
