Assuntos
Psoríase/genética , Receptores KIR3DL1/genética , Adulto , Idade de Início , Estudos de Casos e Controles , Epitopos/genética , Epitopos de Linfócito T/metabolismo , Feminino , Antígenos HLA-B/metabolismo , Humanos , Masculino , Receptores KIR3DL1/antagonistas & inibidores , Fatores de RiscoRESUMO
BACKGROUND: Basal cell carcinoma (BCC) is characterized by a low rate of metastasis, slow growth and strong stroma dependency, with significant morbidity and public health burden. Cancer-testis (CT) genes are specifically expressed in normal testis, fetal ovary and different types of cancers. Testis immune privileged status makes CT genes promising candidates as cancer markers, vaccines and immunotherapy. OBJECTIVES: To find new CT genes as cancer markers and candidate genes for immunotherapy and to correlate pathological and clinical features with their expression in patients with BCC. METHODS: By means of digital differential display, seven testis-specific genes were selected. Their expression patterns were analysed in 78 BCC and 15 normal skin samples using semiquantitative reverse transcription-polymerase chain reaction. Pathological and clinical characteristics were determined using appropriate methods. RESULTS: SPATA19, TEX101, ODF1, ODF2 and ODF3 were expressed in 56.6%, 38.2%, 2.6%, 17.4% and 2.6% of BCCs but not in normal skin samples. ODF4 and PASD1 were not expressed in any BCC samples. TEX101 and SPATA19 expression in high-risk BCCs was higher than in low-risk tumours (P < 0.001). SPATA19 expression was correlated with a history of cancer radiotherapy (P < 0.001). Significant associations were found between expression of TEX101 with nodular subtype, ODF2 with infiltrating subtype, and ODF1 with tumours located on the neck. Among gene expressors, 42.1% co-expressed two genes and 5.3% co-expressed three genes. CONCLUSIONS: We report five new CT antigens, of which SPATA19 and TEX101 may be possible targets for cancer immunotherapy and novel markers for early detection of BCC.
Assuntos
Carcinoma Basocelular/genética , Proteínas de Choque Térmico/genética , Proteínas Mitocondriais/genética , Proteínas de Plasma Seminal/genética , Neoplasias Cutâneas/genética , Testículo/imunologia , Análise de Variância , Antígenos de Neoplasias/genética , Antígenos Nucleares/genética , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/imunologia , Carcinoma Basocelular/imunologia , Carcinoma Basocelular/patologia , Feminino , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Genes Neoplásicos/genética , Proteínas de Choque Térmico/imunologia , Humanos , Masculino , Proteínas Mitocondriais/imunologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Proteínas de Plasma Seminal/imunologia , Neoplasias Cutâneas/imunologiaRESUMO
BACKGROUND: Subcision is a procedure that has been reported to be beneficial in treatment of acne scars. OBJECTIVE: This stady aims to assess the efficacy of subcision in the treatment of rolling acne scars and evaluate a novel subdermal filler 'absorbable plain catgut suture' with subcision. METHODS: Twenty-two patients with rolling acne scars underwent subcision, 20 of whom completed treatment and follow-up period. One side of the face underwent subcision and another side subcision with subdermal implant. The patients and investigators' assessment of improvement were both recorded. RESULTS: Subcision showed mild improvement in about 60% of patient and moderate improvement in about 40% of them. The rate of response showed no significant difference with the use of subdermal implant. The side-effects of local oedema, bruising and infection were all transient. CONCLUSIONS: Subcision seems to be a safe method to correct the rolling acne scars with long-term improvement. However, the subdermal implant led to no significant superior results.
