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3.
Artigo em Russo | MEDLINE | ID: mdl-3756249

RESUMO

A dynamic structure of interphase nucleus--perinucleolar zone has been detected and studied in cell systems on high-level transcription and proliferation activity by means of light and fluorescent microscopy methods. A new light-microscopic level classification of nucleoli taking into account the structure of this zone is introduced. The percentage of nucleoli type distribution may be used as criterion of tissue synthesis activity. An introduction of a new morphological criterion in classical Smetana nucleolar test is under discussion.


Assuntos
Nucléolo Celular/ultraestrutura , Núcleo Celular/ultraestrutura , Nucléolo Celular/classificação , Nucléolo Celular/efeitos dos fármacos , Núcleo Celular/efeitos dos fármacos , Fabaceae/ultraestrutura , Células HeLa/ultraestrutura , Humanos , Interfase/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Linfócitos/ultraestrutura , Microscopia/métodos , Microscopia de Fluorescência/métodos , Fito-Hemaglutininas/farmacologia , Lectinas de Plantas , Plantas Medicinais , Transcrição Gênica/efeitos dos fármacos , Tuberculina/farmacologia
4.
Biofizika ; 26(5): 834-8, 1981.
Artigo em Russo | MEDLINE | ID: mdl-7032606

RESUMO

Complex investigations of plant and animal cells showed the nucleolus as a compartmental pulsatile system with the cycle of about one hour. Two main constitutions of the nucleolus are revealed by vital cytochemical methods: lipid-positive and lipid-negative ones. A new model of transcription is suggested. According to this model the nucleolus is a place of endoplasmic reticulum formation. The programmed endoplasmic reticulum goes out to the cytoplasm through a special channel just as the nucleolus contracts.


Assuntos
Nucléolo Celular/fisiologia , Retículo Endoplasmático/fisiologia , Animais , Nucléolo Celular/metabolismo , Nucléolo Celular/ultraestrutura , Células Cultivadas , Histocitoquímica , Metabolismo dos Lipídeos , Camundongos , Microscopia Eletrônica , Microscopia de Contraste de Fase , Plantas
10.
Tsitologiia ; 17(4): 470-2, 1975 Apr.
Artigo em Russo | MEDLINE | ID: mdl-49964

RESUMO

The localization and frequency of accurrence of Y-chromosome fluorescent bodies in interphase nuclei in the cellular culture of 4-months male and female human embryos, were studied with fluorochromine method with quinacrine dehydrochloride. In cellular cultures of male embryonic kidney the frequency of Y-chromatin was about 50%. The Y-chromosome structural connection with nuclear membrane and nucleolus was demonstrated.


Assuntos
Cromatina , Mitose , Cromossomos Sexuais , Núcleo Celular , Células Cultivadas , Feminino , Humanos , Rim/embriologia , Masculino , Coloração e Rotulagem
11.
Tsitol Genet ; 9(2): 99-100, 1975.
Artigo em Russo | MEDLINE | ID: mdl-1129862

RESUMO

The results obtained testify to the dependence of the interpretation of the interphase nucleus structure on the nucleus axis orientation respective to preparation plane. Variations in arrangement of nucleolus and perinucleolar vacuole with respect to the achromatic pole and centromeric ring are demonstrated.


Assuntos
Cromossomos , Mitose , Células Vegetais
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