RESUMO
The rs3129882, a noncoding variant in HLA-DR, was found to be associated with Parkinson's disease (PD) using several genome-wide association studies. The aim of this replication study was to explore the relationship between this variant and PD in Iranian population. Genomic DNA was extracted from peripheral blood samples, and the rs3129882 SNP was genotyped using a PCR-RFLP method in 520 PD patients and 520 healthy Iranian controls. Significant differences were found in allele frequencies between patients and controls (χ(2) = 4.64, P = 0.031). Under additive and dominant models, the association of the SNP with PD risk is significant, where the A allele was observed to be protective. The results suggest that rs3129882 polymorphism may be a risk factor for PD in Iranian. This is the first study reporting such an association in this population. More replication studies are needed to confirm this data.
Assuntos
Estudos de Associação Genética , Predisposição Genética para Doença , Cadeias alfa de HLA-DR/genética , Doença de Parkinson/genética , Alelos , Estudos de Casos e Controles , Feminino , Frequência do Gene/genética , Humanos , Padrões de Herança/genética , Irã (Geográfico) , Masculino , Pessoa de Meia-Idade , Modelos Genéticos , Polimorfismo de Nucleotídeo Único/genéticaRESUMO
In this study Trichoderma atroviride was selected as over producer of chitinase enzyme among 30 different isolates of Trichoderma sp. on the basis of chitinase specific activity. From this isolate the genomic and cDNA clones encoding chit33 have been isolated and sequenced. Comparison of genomic and cDNA sequences for defining gene structure indicates that this gene contains three short introns and also an open reading frame coding for a protein of 321 amino acids. The deduced amino acid sequence includes a 19 aa putative signal peptide. Homology between this sequence and other reported Trichoderma Chit33 proteins are discussed. The coding sequence of chit33 gene was cloned in pEt26b(+) expression vector and expressed in E. coli.
Neste estudo Trichoderma atroviride foi escolhido como superprodutor da enzima quitinase dentre 30 isolados de Trichoderma sp. com base na atividade específica de quitinase. Clones de cDNA e genômico codificando chit33 foram obtidos deste isolado e seqüenciados. A comparação das seqüências genômica e de cDNA para definir a estrutura do gene indicou que este contém três pequenos introns e uma fase aberta de leitura codificando uma proteína de 321 aminoácidos. A seqüência de aminoácidos deduzida inclui um possível peptídio sinal de 19 aminoácidos. Homologia entre esta seqüência e outras proteínas Chit33 descritas de Trichoderma é discutida. A seqüência codificadora do gene chit33 foi clonada no vetor de expressão pET26b(+) e expressa em E. coli.
Assuntos
Clonagem Molecular , Técnicas In Vitro , Inteínas , Quitinases/análise , Trichoderma/genética , Trichoderma/isolamento & purificação , Sequência de Aminoácidos , Métodos , Estrutura Molecular , MétodosRESUMO
In this study Trichoderma atroviride was selected as over producer of chitinase enzyme among 30 different isolates of Trichoderma sp. on the basis of chitinase specific activity. From this isolate the genomic and cDNA clones encoding chit33 have been isolated and sequenced. Comparison of genomic and cDNA sequences for defining gene structure indicates that this gene contains three short introns and also an open reading frame coding for a protein of 321 amino acids. The deduced amino acid sequence includes a 19 aa putative signal peptide. Homology between this sequence and other reported Trichoderma Chit33 proteins are discussed. The coding sequence of chit33 gene was cloned in pEt26b(+) expression vector and expressed in E. coli.
RESUMO
In this study the effect of two plant growth regulators (indolacetic acid, IAA and gibberellic acid, GA3) and also Trichoderma harzianum (T8) on the phytopathogen fungus Fusarium oxysporium (F15) was investigated. IAA and GA3 with 15 and 30 ppm concentration have no significant effect on T. harzianum (T8) growth. The biocontrol activity of T. harzianum on F. oxysporum was slightly decreased by the presence of IAA and/or GA3. Addition of 40 ppm of GA3 to the culture medium of F. oxsporum increased polygalacturonase activity about 100%. A strong increasing effect on chitinase activity (60%) by T. harzianum (T8) was observed in the presence of phytopathogenic fungus F. oxysporum, but 40 ppm IAA and/or GA3 decreased about 47% of chitinase activity of T. harzianum.
Assuntos
Fusarium/metabolismo , Controle Biológico de Vetores/métodos , Doenças das Plantas/microbiologia , Reguladores de Crescimento de Plantas/farmacologia , Trichoderma/metabolismo , Antifúngicos/farmacologia , Quitinases/química , Quitinases/metabolismo , Modelos Biológicos , Micélio/metabolismo , Poligalacturonase/química , Esporos Fúngicos/metabolismo , Trichoderma/crescimento & desenvolvimentoRESUMO
Pathogenic Yersinia enterocolitica harbour plasmid that is essential for virulence. We studied the characteristics of virulence plasmid using serological, biochemical and bioassay tests in Y. enterocolitica isolates of chicken using plasmid curing. Plasmid-cured isogenic derivatives (2029c and 2150c) were obtained from two isolates of Y. enterocolitica (RTCC 2029 and RTCC 2150). The results demonstrated that plasmid-bearing isolates (2029 and 2150) were human-serum-resistant when grown at 37 degrees C, but were sensitive when grown at 25 degrees C, whereas plasmid-cured isolates (2029c and 2150c) were sensitive when grown at both temperatures. Also autoagglutination, calcium-dependency tests and experimental infection in mice demonstrated that these phenotypes were associated with the virulence plasmid.
Assuntos
Galinhas , Plasmídeos/genética , Doenças das Aves Domésticas/microbiologia , Yersiniose/microbiologia , Yersiniose/veterinária , Yersinia enterocolitica/patogenicidade , Animais , Técnicas Bacteriológicas , Modelos Animais de Doenças , Irã (Geográfico) , Camundongos , Fenótipo , Doenças das Aves Domésticas/sangue , Sorotipagem , Temperatura , Yersiniose/sangue , Yersinia enterocolitica/classificação , Yersinia enterocolitica/isolamento & purificação , Yersinia enterocolitica/fisiologiaRESUMO
Pathogenic Yersinia enterocolitica harbour plasmid that is essential for virulence. We studied the characteristics of virulence plasmid using serological, biochemical and bioassay tests in Y. enterocolitica isolates of chicken using plasmid curing. Plasmid-cured isogenic derivatives [2029c and 2150c] were obtained from two isolates of Y. enterocolitica [RTCC 2029 and RTCC 2150]. The results demonstrated that plasmid-bearing isolates [2029 and 2150] were human-serum-resistant when grown at 37 ّC, but were sensitive when grown at 25 ّC, whereas plasmid-cured isolates [2029c and 2150c] were sensitive when grown at both temperatures. Also autoagglutination, calcium-dependency tests and experimental infection in mice demonstrated that these phenotypes were associated with the virulence plasmid
