RESUMO
A total of 15 isolates of Salmonella enterica serovar 4,5,12:i:- obtained from diseased cows and patients in Iwate Prefecture, Japan, were characterized to clarify the genetic basis of this serovar. S. Typhimurium- specific IS200 was detected from all the isolates. A 94-kb plasmid and the spvB gene were detected from all but one of the 15 isolates. The results of PCR mapping of the fljAB operon and its flanking regions indicate that there are deletions or mutations in this chromosomal region. These data suggest that the 15 isolates are monophasic variants of S. Typhimurium. Epidemiological relationships between the isolates obtained from cattle and humans were not suspected based on the comparison of data employing plasmid profiling and pulsed-field gel electrophoresis.
Assuntos
Doenças dos Bovinos/microbiologia , Salmonelose Animal/microbiologia , Salmonella enterica/classificação , Zoonoses/microbiologia , ADP Ribose Transferases/química , ADP Ribose Transferases/genética , Animais , Bovinos , Doenças dos Bovinos/genética , DNA Bacteriano/química , DNA Bacteriano/genética , Eletroforese em Gel de Campo Pulsado/veterinária , Humanos , Japão , Testes de Sensibilidade Microbiana/veterinária , Reação em Cadeia da Polimerase/veterinária , Polimorfismo de Fragmento de Restrição , Salmonelose Animal/genética , Salmonella enterica/genética , Fatores de Virulência/química , Fatores de Virulência/genéticaRESUMO
The E2 regions of 177 bovine viral diarrhea virus (BVDV) strains isolated in Japan between 1957 and 2006 were analyzed for genotyping. The strains were classified into 8 genotypes (1a, 1b, 1c, 1d, 1e, 1f, So and 2a) based on the phylogenetic analysis. The restriction fragment length polymorphism (RFLP) analysis of the RT-PCR products using 6 selected enzymes (Apo I, Mly I, BstAP I, Pvu II, Ear I, EcoR V) disclosed the cutting patterns classified into 11 groups (I-XI), each of that consisted of strains belonging to a single genotype. Namely, groups-I and -II were composed by genotype-1a strains, groups-III and -IV by 1b strains, and groups-V and -VI by 1c strains. Other groups-VII, -VIII, -IX, -X and -XI comprised genotypes-1d, -1e, -1f, -So and -2a strains, respectively. The results suggest that the RFLP analysis can simply and rapidly differentiate the 8 genotypes of BVDV strains.
Assuntos
Vírus da Diarreia Viral Bovina/classificação , Vírus da Diarreia Viral Bovina/genética , Genótipo , Polimorfismo de Fragmento de Restrição , Animais , Japão , Reação em Cadeia da Polimerase/veterináriaRESUMO
To detect herds including cattle persistently infected (PI) with bovine viral diarrhea virus (BVDV), application of the combination of neutralizing antibody detection and virus isolation, so-called spot test, were performed on sera of 3 calves selected from each of 26 farms. Nine farms were judged as positive because 64 or more antibody titers were detected from 2 or more calves or BVDV was isolated from one or more calves. PI cattle were detected from 8 of the 9 farms. The positive judgment on one farm was obtained only when the indicator virus used on the neutralizing test was genotypically identical with the isolate from the farm. These results suggest that the spot test can be effective in detecting herds with PI cattle and that the accuracy may be influenced by the genotypes of the indicator viruses.
