RESUMO
Using polymorphism of alpha-amylase in the winter common wheat studied inheritance isoenzymes and its conjugation enzyme types with germinating grain on the "vine", grain productivity, plant height and time of ear formation. It is shown that the polymorphism isoenzyme of alpha-amylase wheat is limited by the presence of different loci whose products are similar in electrophoretic parameters. In this regard, one component of the enzyme can be controlling at one or two or three genes. Identification of a locus controlling alpha-amylase isoenzyme in the fast moving part of the electrophoretogram, designated as α-Amy-B7. Determine the distance of the locus to factor α-Amy-B6.
Assuntos
Polimorfismo Genético , Locos de Características Quantitativas , Triticum/enzimologia , Triticum/genética , alfa-Amilases/genética , Eletroforese em Gel de Poliacrilamida , Ligação Genética , Genoma de Planta , Isoenzimas/genética , Sementes/enzimologia , Sementes/genéticaRESUMO
The polymorphism of winter common wheat with respect to ß-amylase isoenzymes has been analyzed using electrophoresis in polyacrylamide gel (PAAG) buffered with a Tris-glycine system (pH 8.3). Seven ß-amylase isoenzymes have been found in wheat cultivars and the breeding stock. Isoenzymes A, B, and C are the most frequent in Russian and Ukrainian cultivars (51.7 4.7, 30.7 3.8, and 11.9 2.5%, respectively). Two alleles of the ß-Amy-D1 locus of the long arm of chromosome 4D have been identified. The substrate-enzyme affine effect can be used to locate the zones of activity of this enzyme by means of staining for proteins. It has been determined that ß-amylase zymotypes may play a role in the aggregating capacity of the grain protein complex via the formation of S-S bonds.