New models of care and multidimensional solutions for oncological patients in the post-acute SARS-COV-2 period: a "Second Phase" also for cancer patients.

In Italy, SARS-CoV-2 outbreak registered a high transmission and disease rates. During the acute phase, oncologists provided to re-organize services and prioritize treatments, in order to limit viral spread and to protect cancer patients. The progressive reduction of the number of infections has prompted Italian government to gradually loosen the national confinement measures and to start the "Second phase" of measures to contain the pandemic. The issue on how to organize cancer care during this post-acute SARS-CoV-2 phase appears crucial and a reassessment of healthcare services is needed requiring new models of care for oncological patients. In order to address major challenges in cancer setting during post-acute SARS-CoV-2 phase, this work offers multidimensional solutions aimed to provide a new way to take care of cancer patients.

Risk of foot and mouth disease spread through cattle movements in Uganda.

Foot and mouth disease (FMD) is endemic in Uganda, where livestock movements through porous borders and beyond play a key role in the spread of transboundary animal diseases. Data from published and unpublished sources were used to conduct a qualitative risk assessment based on the World Organisation for Animal Health framework to assess the risk of foot and mouth disease virus spread in Uganda through pastoral and trade-related cattle movements from the country's southern border districts. A scenario tree was developed as a conceptual framework, and the risk was assessed by considering factors including the cattle population, proportion of vaccinated cattle, number of live cattle legally moved from districts along the Ugandan-Tanzanian border, the production system in the destination districts and the purpose of the movement. Factors associated with higher risk included live cattle movements for pastoral/grazing and breeding purposes, particularly those towards agro-pastoral (AP) areas, which have the potential to lead to outbreaks on several farms in the destination district and other districts countrywide. Prophylactic vaccination should therefore prioritise districts from which movements of large volumes of cattle to other areas originate and the AP destination districts. Specific awareness campaigns should be conducted in destination districts to improve preventative measures and farm biosecurity levels. This study will inform the revision of the risk-based strategic plan, aimed at reducing FMD impacts in Uganda, as the country progresses along the progressive control pathway for FMD.

La fièvre aphteuse est présente à l'état endémique en Ouganda, pays où les mouvements de bétail à travers et au-delà des frontières poreuses jouent un rôle déterminant dans la propagation des maladies animales transfrontalières. Une évaluation qualitative des risques basée sur le cadre de l'Organisation mondiale de la santé animale a été réalisée, en utilisant des données provenant aussi bien de sources publiées que non publiées, afin d'évaluer les risques de propagation du virus de la fièvre aphteuse en Ouganda par le biais des mouvements pastoraux et commerciaux de bétail en provenance des districts frontaliers du sud du pays. Un arbre de scénarios a été élaboré en tant que cadre conceptuel. Les risques ont été évalués en tenant compte de facteurs tels que les effectifs du cheptel bovin, la proportion de bovins vaccinés, le nombre de bovins vivants déplacés légalement depuis les districts situés le long de la frontière entre l'Ouganda et la Tanzanie, le système de production pratiqué dans les districts de destination et la finalité des déplacements du bétail. Les principaux facteurs associés à un risque accru étaient les mouvements de bovins vivants liés à l'élevage pastoral/ la mise en pâturage ou à des fins de reproduction, et plus particulièrement les déplacements vers les zones agro-pastorales, en raison du potentiel épidémique qu'ils peuvent avoir dans les fermes du district de destination et d'autres districts à l'échelle du pays. La vaccination prophylactique devrait donc être conduite en priorité dans les districts de provenance des bovins déplacés en grand nombre vers d'autres zones, ainsi que dans les districts de destination lorsqu'ils sont à dominante agro-pastorale. Des campagnes spécifiques d'information et de sensibilisation devraient être menées dans les districts de destination afin d'améliorer les mesures de prévention et le niveau de biosécurité des élevages. Les résultats de cette étude étayeront la mise à jour du plan stratégique fondé sur les risques, qui vise à réduire l'impact de la fièvre aphteuse en Ouganda parallèlement aux avancées du pays sur la voie de l'approche progressive de la lutte contre la fièvre aphteuse.

La fiebre aftosa es endémica en Uganda, país donde los desplazamientos de ganado a través y más allá de sus porosas fronteras son un factor decisivo en la propagación de enfermedades animales transfronterizas. Los autores exponen una evaluación cualitativa del riesgo realizada a partir de datos publicados e inéditos con empleo del marco de la Organización Mundial de Sanidad Animal. Se trataba de evaluar así el riesgo de propagación del virus de la fiebre aftosa en Uganda a resultas de los desplazamientos de ganado desde los distritos fronterizos meridionales con fines de pastoreo o de comercio. Tras elaborar como marco teórico un árbol de hipótesis, se determinó el riesgo teniendo en cuenta, como principales factores, la cabaña bovina, la proporción de ejemplares vacunados, el número de animales vivos transportados legalmente desde los distritos que bordean la frontera entre Uganda y Tanzania, el sistema productivo en los distritos de destino y la finalidad de cada desplazamiento. Entre los factores ligados a un aumento del riesgo destacaba el desplazamiento de animales vivos con fines de pastoreo y de reproducción, en particular con destino a zonas agropastorales, pues ello puede provocar brotes en múltiples explotaciones no solo del distrito de destino, sino también de otros distritos de todo el país. En las actividades de vacunación profiláctica, por lo tanto, conviene otorgar prioridad a los distritos de los que parten grandes contingentes de ganado hacia otras zonas y también a los distritos de destino agropastorales. También habría que implantar campañas específicas de sensibilización en los distritos de destino para mejorar en ellos las medidas de prevención y los niveles de seguridad biológica de las explotaciones. Este estudio servirá de base para la revisión del plan estratégico basado en los riesgos, encaminado a reducir las repercusiones de la fiebre aftosa en Uganda, a la par que el país va cubriendo etapas en la senda progresiva de control de la fiebre aftosa.

Pregnenolone and pregnenolone-methyl-ether rescue neuronal defects caused by dysfunctional CLIP170 in a neuronal model of CDKL5 Deficiency Disorder.

Mutations in the X-linked cyclin-dependent kinase-like 5 (CDKL5) gene are responsible for the onset of CDKL5 Deficiency Disorder (CDD), a neurological pathology characterised by severe infantile seizures, intellectual disability, impairment of gross motor skills, sleep and gastrointestinal disturbances. CDKL5 is a serine/threonine kinase the molecular network of which is not yet fully understood. Loss of CDKL5 both in vitro and in vivo leads to altered neuronal morphology including axon specification and outgrowth, dendritic arborisation and spine morphology suggesting a link between CDKL5 and the regulation of proper cytoskeleton functioning. Recently, we found that CDKL5 regulates the binding of CLIP170 to microtubules (MT). CLIP170 is a MT-plus end tracking protein (+TIP) that associates with MTs when present in its open, active conformation. Here we present evidence suggesting CLIP170 contributes to neuronal CDKL5-dependent defects and that it represents an important novel druggable target for CDD; indeed, CLIP170 is directly targeted by the neuroactive steroid pregnenolone (PREG), which induces the active conformation of the protein thus promoting MT-dynamics. We here show that PREG and a synthetic derivative pregnenolone-methyl-ether (PME) can restore the MT association of CLIP170 and revert morphological and molecular defects in Cdkl5-KO neurons at different stages of maturation. All together, these findings identify CLIP170 as possible novel druggable target for CDKL5 related disorders providing an intriguing prospective for future disease-modifying drug-based therapies.

Liquid chromatography-tandem mass spectrometry for the simultaneous quantitation of enmetazobactam and cefepime in human plasma.

A simple ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous analysis enmetazobactam (also known as AAI101) and cefepime in human plasma. Sample preparation was based on protein precipitation with acetonitrile. Separation was performed on Acquity BEH HILIC column (50 mm × 2.1 mm, 1.7 µm) with a mobile phase containing ammonium formate in water and acetonitrile. The analytes were analyzed with the corresponding isotopically labeled internal standards and were detected in multiple reactions monitoring (MRM) using API 5000 triple-quadrupole mass spectrometer with electrospray (ESI) source operating in positive ion mode. The calibration curves were linear over the selected ranges (r > 0.9970 for both analytes). The intra and inter-assay precision of the Quality Control samples showed CV ≤ 15% and the accuracy was within 85 and 115% in all cases for both compounds. The lower limit of quantification was 0.05 µg/mL for enmetazobactam and 0.5 µg/mL for cefepime.

Teeth penetration force of the tiger shark Galeocerdo cuvier and sandbar shark Carcharhinus plumbeus.

This study examined the minimum force required of functional teeth and replacement teeth in the tiger shark Galeocerdo cuvier and the sandbar shark Carcharhinus plumbeus to penetrate the scales and muscle of sheepshead Archosargus probatocephalus and pigfish Orthopristis chrysoptera. Penetration force ranged from 7·7-41·9 and 3·2-26·3 N to penetrate A. probatocephalus and O. chrysoptera, respectively. Replacement teeth required significantly less force to penetrate O. chrysoptera for both shark species, most probably due to microscopic wear of the tooth surfaces supporting the theory shark teeth are replaced regularly to ensure sharp teeth that are efficient for prey capture.

Transgenerational effects of a hypercaloric diet.

The effects of a maternal hypercaloric diet (HD) during puberty and early adulthood on neuroimmune aspects in offspring were investigated. In female rats of the F0 generation and male rats of the F1 generation, bodyweight (BW) gain, retroperitoneal fat (RPF) weight, the number of hypodermic adipocytes (HAs) and expression of glial fibrillary acidic protein (GFAP) were measured in hypothalamic astrocytes. On Postnatal Day 50, the F1 pups were challenged with lipopolysaccharide (LPS, 100µgkg-1, s.c.) or an equal volume of saline (S), and behaviour in the open field test was evaluated, as were plasma neuropeptide and cytokine concentrations. The maternal HD caused the female F0 rats to become overweight. The F1 offspring of dams fed the HD and challenged with saline (HDS group) exhibited increases in BW gain, RPF weight and in the number of large HAs and a decrease in GFAP immunoreactivity. F1 offspring of dams fed the HD and challenged with LPS (HDLPS group) exhibited decreases in BW gain, RPF weight and GFAP immunoreactivity, but no differences were observed in the number of larger and small HAs. Plasma tumour necrosis factor-α concentrations were high in the HDS and HDLPS groups. Thus, the maternal HD during puberty and early adulthood caused the F1 generation to become overweight despite the fact that they received a normocaloric diet. These results indicate a transgenerational effect of the HD that may occur, in part, through permanent changes in immune system programming. The attenuation of neuroinflammation biomarkers after LPS administration may have resulted in a decrease in the number of adipocytes, which, in turn, reduced cytokine, adipokine and chemokine levels, which are able to recruit inflammatory cells in adipose tissue.

Maternal food restriction in rats of the F0 generation increases retroperitoneal fat, the number and size of adipocytes and induces periventricular astrogliosis in female F1 and male F2 generations.

The present study investigated whether male offspring (F2 generation) from female rats (F1 generation) whose mothers (F0 generation) were food restricted during gestation inherit a phenotypic transgenerational tendency towards being overweight and obese in the juvenile period, in the absence of food restriction in the F1/F2 generations. Dams of the F0 generation were 40% food restricted during pregnancy. Bodyweight, the number and size of larger and small hypodermal adipocytes (HAs), total retroperitoneal fat (RPF) weight and the expression of glial fibrillary acidic protein (GFAP) in periventricular hypothalamic astrocytes (PHAs), as determined by immunohistochemistry, were evaluated in both generations. In the female F1 generation, there was low bodyweight gain only during the juvenile period (30-65 days of age), a decrease in the size of small adipocytes, an increase in the number of small adipocytes, an increase in RPF weight and an increase in GFAP expression in PHAs at 90-95 days of age. In males of the F2 generation at 50 days of age, there was increased bodyweight and RPF weight, and a small number of adipocytes and GFAP expression in PHAs. These data indicate that the phenotypic transgenerational tendency towards being overweight and obese was observed in females (F1) from mothers (F0) that were prenatally food restricted was transmitted to their male offspring.

Complexity of care: a concept analysis.

BACKGROUND: In spite of the high number of tools designed to measure the complexity of care, there is still great diversity in the meaning of this concept. METHODS: The study was carried out using the concept analysis method as described by Beth Rodgers; 27 international papers were selected using PubMed, Web of Science and CINAHL data sets, without any time constraints. RESULTS: A number of similar concepts relating to multiplicity, intensity of care and workload were selected. The antecedents were classified according to personal and clinical features of patients, the characteristics of care, the social and organizational features; the tools that emerged measure the risk of complexity of care. Among the consequences, those that emerged were related to patients, operators and organization. The two attributes of complexity of care are connected with measurement, on the one side, and uncertainly, on the other. CONCLUSIONS: As difficult as it is to define complexity of care, the analysis states that its classification should be targeted at redefining hospital organization.

Comparative squamation of the lateral line canal pores in sharks.

The current study collected the first quantitative data on lateral line pore squamation patterns in sharks and assessed whether divergent squamation patterns are similar to experimental models that cause reduction in boundary layer turbulence. In addition, the hypothesis that divergent orientation angles are exclusively found in fast-swimming shark species was tested. The posterior lateral line and supraorbital lateral line pore squamation of the fast-swimming pelagic shortfin mako shark Isurus oxyrinchus and the slow-swimming epi-benthic spiny dogfish shark Squalus acanthias was examined. Pore scale morphology and pore coverage were qualitatively analysed and compared. In addition, pore squamation orientation patterns were quantified for four regions along the posterior lateral line and compared for both species. Isurus oxyrinchus possessed consistent pore scale coverage among sampled regions and had a divergent squamation pattern with multiple scale rows directed dorsally and ventrally away from the anterior margin of the pore with an average divergent angle of 13° for the first row of scales. Squalus acanthias possessed variable amounts of scale coverage among the sampled regions and had a divergent squamation pattern with multiple scale rows directed ventrally away from the anterior margin of the pore with an average angle of 19° for the first row of scales. Overall, the squamation pattern measured in I. oxyrinchus fell within the parameters used in the fluid flow analysis, which suggests that this pattern may reduce boundary layer turbulence and affect lateral line sensitivity. The exclusively ventral oriented scale pattern seen in S. acanthias possessed a high degree of divergence but the pattern did not match that of the fluid flow models. Given current knowledge, it is unclear how this would affect boundary layer flow. By studying the relationship between squamation patterns and the lateral line, new insights are provided into sensory biology that warrant future investigation due to the implications for the ecology, morphology and sensory evolution of sharks.

Stemmed versus stemless total shoulder arthroplasty: a preliminary report and short-term results.

BACKGROUND: Although several studies have been performed on the use of various devices in total shoulder arthroplasty (TSA), no data are available in order to establish whether to prefer stemmed or stemless humeral components. Thus, the purpose of our study was to evaluate the short-term functional outcome in a cohort of subjects treated with TSA randomized to treatment with stemmed or stemless prosthesis. METHODS: In this prospective longitudinal study, we randomized to treatment with stemmed (group 1) or with stemless (group 2) humeral component in nineteen subjects (2 M and 17 F) diagnosed with humeral primary osteoarthritis with indication to TSA. We evaluated the range of movement of all the participants and the functional outcome using Constant score and simple shoulder test (SST) before and after 2 years from surgery. RESULTS: No differences were detected after 2 years from surgery in the two groups in terms of functional scores and range of motion (p > 0.05). CONCLUSION: Stemmed and stemless prostheses are comparable in terms of functional outcome. These data might be useful for the surgeon in order to choose more tissues-paring methodologies and less invasive procedures, such as stemless humeral implants.

Avaliação da nested PCR em comparação aos testes sorológicos IDGA e ELISA para o diagnóstico da anemia infecciosa equina / Evaluation of nested PCR compared with AGID and ELISA serological tests for equine infectious anemia diagnosis

Comparou-se a técnica nested PCR (nPCR) com os testes sorológicos IDGA e ELISA para o diagnóstico da anemia infecciosa equina. Amostras do DNA provenientes das células mononucleares do sangue periférico foram submetidas à amplificação do gene gag pela nPCR, que apresentou valores de sensibilidade e especificidade relativas de 90 por cento e 52,9 por cento, respectivamente, em relação à IDGA, e valores de 85,7 por cento e 49 por cento, respectivamente, em relação ao ELISA. Considerando-se os fatores referentes às limitações de cada técnica, pode ser sugerido o uso da nPCR como teste de diagnóstico complementar para AIE em amostras brasileiras.

The nested polymerase chain reaction (nPCR) technique was compared to AGID and ELISA serological tests for the diagnosis of Equine Infectious Anemia. DNA samples from the peripheral blood mononuclear cells were subjected to the amplification of the gag gene by nPCR, which showed relative sensibility and specificity values of 90.0 percent and 52.9 percent respectively, compared to the AGID and values of 85.7 percent and 49.0 percent, respectively, as compared to ELISA. Considering the factors concerning the limitations of each technique, the use of nPCR can be suggested as a complementary diagnostic test for EIA in Brazilian samples.

Individual monitoring in nuclear medicine therapeutic procedures using extremity dosemeters LiF(Mg, Cu, P).

Unsealed beta-gamma-emitting sources are used (15 GBq (90)Y each session) in nuclear medicine therapeutic procedures. Inside the manipulation cell and while giving the injection to the patient, the skin exposure is very high; electron radiation field is not homogeneous and thus the exposure of the extremities is not uniform. Particular individual monitoring is adopted: single thermoluminescence dosemeter, wrapped in polyethylene film and placed on an adhesive tape, is positioned on the tip of the fingers; 6-10 dosemeters are assigned to each operator per session. The energy and angle response is studied for X-ray spectra, (90)Sr/Y and (204)Tl--a unique mean calibration factor is calculated in order to estimate H(p)(0.07). Performance of dosemeter is analysed according to ISO 62387-1(2007) and the combined uncertainty (calculated using the Monte Carlo method) results lie in the order of 11 %. This method reveals the critical step of manipulation and administration and ensures that dose limits are not exceeded.

Comparison of three methods of DNA extraction from peripheral blood mononuclear cells and lung fragments of equines.

We compared three different protocols for DNA extraction from horse peripheral blood mononuclear cells (PBMC) and lung fragments, determining average final DNA concentration, purity, percentage of PCR amplification using beta-actin, and cost. Thirty-four samples from PBMC, and 33 samples from lung fragments were submitted to DNA extraction by three different protocols. Protocol A consisted of a phenol-chloroform and isoamylic alcohol extraction, Protocol B used alkaline extraction with NaOH, and Protocol C used the DNAzol((R)) reagent kit. Protocol A was the best option for DNA extraction from lung fragments, producing high DNA concentrations, with high sensitivity in PCR amplification (100%), followed by Protocols C and B. On the other hand, for PBMC samples, Protocol B gave the highest sensitivity in PCR amplification (100%), followed by Protocols C and A. We conclude that Protocol A should be used for PCR diagnosis from lung fragment samples, while Protocol B should be used for PBMC.

Bristled shark skin: a microgeometry for boundary layer control?

There exists evidence that some fast-swimming shark species may have the ability to bristle their scales during fast swimming. Experimental work using a water tunnel facility has been performed to investigate the flow field over and within a bristled shark skin model submerged within a boundary layer to deduce the possible boundary layer control mechanisms being used by these fast-swimming sharks. Fluorescent dye flow visualization provides evidence of the formation of embedded cavity vortices within the scales. Digital particle image velocimetry (DPIV) data, used to evaluate the cavity vortex formation and boundary layer characteristics close to the surface, indicate increased momentum in the slip layer forming above the scales. This increase in flow velocity close to the shark's skin is indicative of boundary layer control mechanisms leading to separation control and possibly transition delay for the bristled shark skin microgeometry.

Population genetic structure of Earth's largest fish, the whale shark (Rhincodon typus).

Large pelagic vertebrates pose special conservation challenges because their movements generally exceed the boundaries of any single jurisdiction. To assess the population structure of whale sharks (Rhincodon typus), we sequenced complete mitochondrial DNA control regions from individuals collected across a global distribution. We observed 51 single site polymorphisms and 8 regions with indels comprising 44 haplotypes in 70 individuals, with high haplotype (h = 0.974 +/- 0.008) and nucleotide diversity (pi = 0.011 +/- 0.006). The control region has the largest length variation yet reported for an elasmobranch (1143-1332 bp). Phylogenetic analyses reveal no geographical clustering of lineages and the most common haplotype was distributed globally. The absence of population structure across the Indian and Pacific basins indicates that oceanic expanses and land barriers in Southeast Asia are not impediments to whale shark dispersal. We did, however, find significant haplotype frequency differences (AMOVA, Phi(ST) = 0.107, P < 0.001) principally between the Atlantic and Indo-Pacific populations. In contrast to other recent surveys of globally distributed sharks, we find much less population subdivision and no evidence for cryptic evolutionary partitions. Discovery of the mating and pupping areas of whale sharks is key to further population genetic studies. The global pattern of shared haplotypes in whale sharks provides a compelling argument for development of broad international approaches for management and conservation of Earth's largest fish.

Avaliação do tratamento com isoniazida em bovinos infectados naturalmente pelo Mycobacterium bovis / Evaluation of the treatment with isoniazid carried out in bovines naturally infected with Mycobacterium bovis

Foi adotado o tratamento intermitente da tuberculose com isoniazida em 240 bovinos de um rebanho naturalmente infectado pelo M. bovis. No início do tratamento 36,6 por cento dos animais foram reagentes positivos e 2,9 por cento inconclusivos. Todos os animais do rebanho receberam isoniazida por via oral em doses de 25mg/kg de peso vivo, administrada três vezes por semana, às segundas, quartas e sextas-feiras, durante 10 meses, perfazendo 120 doses. A cura de 98,9 por cento dos animais tratados foi verificada por meio da dessensibilização alérgica realizada pela tuberculinização e controle bacteriológico de 39 animais abatidos. O tratamento neste rebanho não resultou em seleção de cepas resistentes a isoniazida, comprovada pelo teste de sensibilidade a essa droga, realizado em cultura de M. bovis isolada de um animal não curado.

Ecological correlates of polyphenism and gregarious roosting in the grass yellow butterfly Eurema elathea (Pieridae).

Eurema elathea adults were census weekly (1992-1994) in six night-roosts around a forest fragment on a farm, and in two roosts in the urban area of Uberlândia, Minas Gerais, Brazil. Males were grouped in six phenotypic classes. These were based on a range between having a conspicuous wide black bar at the dorsal forewing inner margin (wet season dark morphs) and the absence of that bar (dry season light morphs). The body mass and wing area of co-occurring morphs were compared: differents morphs showed similar means. The abundance of butterflies and morph frequencies varied in close relation to humidity (rainfall). Individuals were infrequent and monomorphically dark in the wet season while light morphs predominated in dry periods when population peaked. A lower fraction of recaptured individuals and higher recruitment were recorded compared to other night-roosting butterflies. Dispersal potential was similar between the sexes and varied seasonally with a more sedentary population in dry periods. The maximum residence time recorded was 91 days for a female and 84 days for a male. The fraction of individuals that moved from one roosting site to another was similar in both sexes and male morphs, but significantly higher on the farm than in the urban area. Also, a significantly higher fraction (21.3%) of marked butterflies was recaptured in the urban area than on the farm (15.6%), suggesting a behavioral modification for sedentariness in the urban individuals. The selective forces shaping a gregarious roosting habit in E. elathea and other butterflies are discussed and a protocooperational strategy for saving energy is proposed.

Ecological correlates of polyphenism and gregarious roosting in the grass yellow butterfly Eurema elathea (Pieridae)

Adultos de Eurema elathea foram estudados semanalmente (1992-1994) em seis locais (dormitórios), ao redor de um fragmento de mata em uma fazenda e em dois locais na área urbana de Uberlândia, MG. Os machos foram classificados em seis categorias fenotípicas, as quais variam desde a presença de uma grande e conspícua barra preta na margem interna dorsal da asa anterior (forma escura da estação úmida) até a ausência da barra (forma clara da estação seca). A massa corporal e a área da asa foram comparadas: formas diferentes mostraram médias similares. A abundância das borboletas e a freqüência das formas variaram conforme a umidade (chuvas). Na estação úmida, os indivíduos foram menos freqüentes e monomorficamente escuros, enquanto na seca, a população aumenta e as formas claras predominam. As taxas de recaptura e recrutamento são comparadas com outras borboletas que se agregam durante a noite. O potencial de dispersão foi similar entre os sexos e variou sazonalmente, sendo que a população é mais sedentária no período seco. O máximo tempo de residência registrado foi de 91 dias para uma fêmea e de 84 dias para um macho. A fração de indivíduos que se moveram de um sítio de descanso para outro foi similar em ambos os sexos e formas de machos, mas foi significativamente maior na fazenda em relação à área urbana. Da mesma forma, foi recapturada uma fração significativamente maior (21,3) de borboletas marcadas na área urbana do que na fazenda (15,6), sugerindo modificação comportamental para o sedentarismo nos indivíduos urbanos. São discutidas as forças seletivas que moldam o hábito de descanso gregário em E. elathea e em outras borboletas, e propõe-se uma estratégia protocooperativa de economia de energia.