Association between myeloperoxidase concentration in equine frozen semen and post-thawing parameters.

Despite improvement of techniques, semen of 20% of stallions remains unfreezable. Recent studies focused on the impact of reactive oxygen species and oxidant enzymes on semen characteristics. Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils during degranulation or after cell lysis. It is responsible for the formation of hypochlorous acid, a strong oxidant agent, which could damage spermatozoa. The aim of this study was to determine the relation between MPO concentration and characteristics of frozen semen from stallions. Thirty-five straws from different stallions were analysed. Post-thawing spermatozoal concentration, and progressive and total motility were determined by Computer-Assisted Semen Analysis. Freezability was determined according to post-thawing progressive motility (above or below 15%). Percentage of alive spermatozoa and abnormal forms was determined after Eosin-Nigrosin and Diff-Quick(®) staining, respectively. Post-thawing MPO concentration was measured by enzyme-linked immunosorbent assay (ELISA). Our study shows that frozen thawed semen contains large amounts of free MPO. We also observed that post-thawing MPO ELISA assay can be used as an indicator of equine semen freezability. High MPO concentration samples showed lower total and progressive motility. A higher proportion of abnormal head shape associated with acrosome reaction was observed in our late examinations of the high concentration MPO group. Our results show that MPO adversely affects total and progressive motility of equine semen. A negative correlation between normal motile forms and MPO concentration was also observed. The effect of MPO on dead or abnormal forms remains to be precised.

[Direct approach for demonstrating free radical phenomena during equine postanesthetic myopathy: preliminary study]. / Approche directe pour la mise en évidence des phénomènes radicalaires lors de myopathie postanesthésique équine: étude préliminaire.

This preliminary study demonstrated the existence of a free radical generation during an experimental postischemic muscular reperfusion in a halothane anesthetized horse. The authors used alpha-phényl-N-tert-butylnitrone as a spin trap agent and the electronic paramagnetic resonance method to observe in vivo a free radical generation.

Equine postanaesthetic myositis: a possible role for free radical generation and membrane lipoperoxidation.

A method for the evaluation of total plasma antihydroxyl and antiperferryl activity is described. This method was applied to horse plasma obtained during halothane anaesthesia. In horses suffering from postanaesthetic myositis, a significant decrease in the antiperferryl activity was observed during anaesthesia particularly when the muscular compression produced by the weight of the horse was released. In the affected muscles, strong oxidants could therefore be generated during the reperfusion of the ischaemic muscles and might initiate membrane lipid peroxidation. This phenomenon could possibly explain the muscular damage observed in equine postanaesthetic myositis.

Equine post anaesthetic myositis: muscular post ischaemic hyperaemia measured by laser Doppler flowmetry.

Measurements of muscular microcirculation in horses anaesthetised with halothane were performed by laser Doppler flowmetry. Variations of microcirculation in the compressed and uncompressed triceps brachii were measured when horses were positioned in dorsal recumbency after a prolonged period in lateral recumbency. A significant post ischaemic hyperaemia was recorded in horses which developed a post anaesthetic myositis.

Equine postanaesthetic myositis: thromboxanes, prostacyclin and prostaglandin E2 production.

Arachidonic acid cyclooxygenase metabolites, thromboxane B2 (TXB2), prostaglandin E2 (PGE2) and 6-keto-prostaglandin F1 (6-keto-PGF1) were measured in horses where anaesthesia was maintained with halothane. Two horses suffering from postanaesthetic myositis were compared with four normal horses. TXB2 and PGE2 levels were higher in mixed venous blood drawn from the myopathic horses. An increase of TXB2 and PGE2 levels appeared when myopathic horses were rolled into dorsal recumbency after a prolonged period of lateral recumbency. One hour after the end of anaesthesia, TXB2 had continued to increase whereas PGE2 decreased. By measurements on blood samples drawn from the brachial vein, we have shown that the rising level of TXB2 in mixed venous blood is mainly due to the increase of TXB2 in blood draining the dependent leg. The origin of the rise in PGE2 is not demonstrated in this study. 6-keto-PGF1 did not change during anaesthesia. An explanation of this imbalance between TXB2 and 6-keto-PGF1 production is considered.

Measurements of muscular microcirculation by laser Doppler flowmetry in isoflurane and halothane anaesthetised horses.

The cardiovascular function of horses was less depressed during anaesthesia with isoflurane than during anaesthesia with halothane. Muscular microcirculation measured by laser Doppler flowmetry was significantly greater in horses anaesthetised with isoflurane.

[Circulatory and respiratory effects of ketamine in horses anesthetized with halothane]. / Effets circulatoires et respiratoires de la kétamine chez le cheval anesthésié à l'halothane.

Ketamine (0.3 mg/kg) administered intravenously to 12 halothane anesthetized horses caused a significant respiratory depression during ten minutes when respiration was spontaneous. Significant hemodynamic effects were not observed except for cardiac index. Clinical application was also discussed.

Laser Doppler flowmetry: muscular microcirculation in anaesthetized horses.

Muscular microcirculation was studied in seven halothane anaesthetised horses in lateral recumbency using a laser Doppler flowmeter. A significant difference between the dependent and the uppermost triceps brachii was recorded. In the dependent muscles, microflow at first decreased and then increased up to the starting value. In the uppermost muscles, a significant rise of the microflow was measured.