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Artigo em Inglês | MEDLINE | ID: mdl-381511

RESUMO

In 33 human sera the determination of diphtheric antitoxic antibodies was performed in a double blind test using Jensen's method, the method of tissue cultures and the haemagglutination method. In the method of tissue cultures the antibody levels in the sera were determinated in the first and second experiment with the precision of +/- half dilution of the geometrical progression. In Jensen's method, the difference between the first and second measurements slightly exceeded +/- 1 dilution. In the haemagglutination method the error considerably exceeded the binary step dilution. In most cases, the determination fluctuated up to seven times the actual value. Differences among the mean values of examination results obtained by Jensen's method and the method of the tissue cultures are statistically insignificant. The differences between the haemagglutination method and both the other methods are statistically significant.


Assuntos
Anticorpos Antibacterianos/biossíntese , Antitoxina Diftérica/imunologia , Análise de Variância , Técnicas de Cultura , Testes de Hemaglutinação , Humanos , Técnicas Imunológicas , Estatística como Assunto
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