RESUMO
The industrial usefulness of the production of foot and mouth disease virus with microcarrier grown cells has been tested at a large scale. The vaccines, intended for pigs, prepared with such virus give a good level of immunity against virulent challenges. They are stable and free from side effects.
Assuntos
Aphthovirus/crescimento & desenvolvimento , Técnicas Citológicas , Vacinas Virais , Cultura de Vírus/métodos , Animais , Aphthovirus/imunologia , Linhagem Celular , Cricetinae , Febre Aftosa/prevenção & controle , Suínos , Doenças dos Suínos/prevenção & controle , Vacinas Virais/imunologia , Vacinas Virais/toxicidadeRESUMO
The characteristics of the viruses produced from cell lines developed in suspension and the vaccines for which they are used should be judged by methods which allow to ensure in all cases their innocuity and efficacity. Innocuity is essentially dependent upon the purity of the viral preparation which should contain a minimum of foreign proteins (due either to the cell or the culture medium) and certain antibiotics. Furthermore, the absence of oncogenic properties of the inactivated viral suspensions should be shown, in addition to their specific innocuity. Efficacity is linked with the concentration of viral particles and with their immunogenic properties after inactivation and incorporation of adjuvants of immunity. Methods of appreciation of the different parameters are proposed.
