Assuntos
Adenocarcinoma Mucinoso/patologia , Neoplasias Palpebrais/patologia , Neoplasias Orbitárias/patologia , Neoplasias Cutâneas/patologia , Adenocarcinoma Mucinoso/cirurgia , Idoso , Neoplasias Palpebrais/cirurgia , Feminino , Humanos , Invasividade Neoplásica , Neoplasias Orbitárias/cirurgia , Neoplasias Cutâneas/cirurgia , Resultado do TratamentoAssuntos
Adenocarcinoma/etiologia , Adenocarcinoma/radioterapia , Terapia com Prótons , Radioterapia de Alta Energia/métodos , Neoplasias da Retina/etiologia , Neoplasias da Retina/radioterapia , Epitélio Pigmentado da Retina/patologia , Adenocarcinoma/patologia , Humanos , Hipertrofia/congênito , Hipertrofia/patologia , Hipertrofia/radioterapia , Masculino , Pessoa de Meia-Idade , Neoplasias da Retina/patologia , Epitélio Pigmentado da Retina/efeitos da radiação , Resultado do TratamentoAssuntos
Neoplasias Oculares/complicações , Neoplasias Oculares/cirurgia , Sarcoma Mieloide/complicações , Sarcoma Mieloide/radioterapia , Esclerite/etiologia , Esclerite/prevenção & controle , Idoso , Neoplasias Oculares/diagnóstico , Humanos , Masculino , Sarcoma Mieloide/diagnóstico , Esclerite/diagnóstico , Resultado do TratamentoAssuntos
Carcinoma Mucoepidermoide/patologia , Neoplasias da Túnica Conjuntiva/patologia , Granuloma/patologia , Idoso , Diagnóstico Diferencial , Humanos , Ceratite/complicações , Ceratite/patologia , Masculino , Doenças da Esclera/complicações , Doenças da Esclera/patologia , Uveíte/complicações , Uveíte/patologiaAssuntos
Neoplasias da Coroide/patologia , Angiofluoresceinografia/métodos , Verde de Indocianina , Leucemia-Linfoma Linfoblástico de Células Precursoras B/patologia , Neoplasias da Retina/patologia , Retinoscopia/métodos , Coriorretinopatia Serosa Central/patologia , Feminino , Humanos , Pessoa de Meia-IdadeRESUMO
BACKGROUND: Inactivation of tumour-related genes by promoter hypermethylation is a common epigenetic event in the development of a variety of tumours. AIM: To investigate in primary uveal melanoma the status of promoter methylation of genes thought to be involved in tumour development: p16, TIMP3, RASSF1, RARB, FHIT, hTERT and APC. METHODS: Gene promoter methylation was studied by methylation-sensitive single-strand conformation analysis and dot-blot assay in a series of 23 primary uveal melanomas. All DNA samples were obtained from paraffin-embedded formalin-fixed tissue blocks. RESULTS: hTERT promoter methylation was found with a relatively high frequency (52%). Promoter methylation of p16, TIMP3, RASSF1, RARB, FHIT and APC was a rare event. For none of these genes did promoter methylation exceed 15% of tumour samples, and, for some genes (FHIT and APC), no methylation was found at all. Furthermore, promoter methylation was absent in 39% (9/23) of cases. In only 22% (5/23) of cases was hypermethylation of at least two promoters observed. CONCLUSIONS: Promoter methylation of hTERT is a regular event in uveal melanoma. Hypermethylation of the other genes studied does not seem to be an essential element in the development of this tumour. As promoter methylation of APC, RASSF1 and RARB is often observed in cutaneous melanoma, these results suggest that different epigenetic events occur in the development of cutaneous and uveal melanoma.
