Assuntos
Atresia Biliar/diagnóstico , Atresia Biliar/fisiopatologia , Doenças do Prematuro/diagnóstico , Doenças do Prematuro/fisiopatologia , Ductos Biliares/anormalidades , Ductos Biliares/diagnóstico por imagem , Ductos Biliares/cirurgia , Atresia Biliar/tratamento farmacológico , Atresia Biliar/cirurgia , Bilirrubina/sangue , Desenvolvimento Infantil , Colagogos e Coleréticos/uso terapêutico , Colestase/etiologia , Cor , Fezes , Feminino , Humanos , Recém-Nascido , Recém-Nascido Prematuro , Doenças do Prematuro/tratamento farmacológico , Doenças do Prematuro/cirurgia , Icterícia Neonatal/etiologia , Portoenterostomia Hepática , Resultado do Tratamento , Ultrassonografia , Ácido Ursodesoxicólico/uso terapêuticoRESUMO
BACKGROUND: IL-2 has been reported to be critical for peripheral T(reg) survival in mouse models. Here, we examined T(reg) maintenance in a series of paediatric liver transplant recipients who received basiliximab, a therapeutic anti-CD25 monoclonal antibody. METHODOLOGY/PRINCIPAL FINDINGS: FoxP3+ CD4 T cells were analyzed by flow cytometry before liver grafting and more than 9 months later. We found that in vivo CD25 blockade did not lead to T(reg) depletion: the proportion of FoxP3+ cells among CD4 T cells and the level of FoxP3 expression were both unchanged. IL-2Rbeta expression was enhanced in FoxP3+ cells both before and after basiliximab treatment, while the level of IL-2Rgamma expression was similar in T(regs) and non-T(regs). No significant change in the weak or absent expression of IL-7Ralpha and IL-15Ralpha expression on FoxP3+ cells was observed. Although the proportion of FoxP3+ cells among CD4 T cells did not vary, food allergies occurred more rapidly after liver grafting in patients who received basiliximab, raising questions as to T(reg) functionality in vivo in the absence of functional CD25. CONCLUSIONS: CD25 appears non essential for human T(reg) peripheral maintenance in vivo. However, our results raise questions as to T(reg) functionality after therapeutic CD25 targeting.
Assuntos
Subunidade alfa de Receptor de Interleucina-2/metabolismo , Linfócitos T Reguladores/metabolismo , Adolescente , Adulto , Idoso , Anticorpos Monoclonais/uso terapêutico , Basiliximab , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/metabolismo , Criança , Pré-Escolar , Feminino , Citometria de Fluxo , Hipersensibilidade Alimentar/tratamento farmacológico , Hipersensibilidade Alimentar/imunologia , Hipersensibilidade Alimentar/metabolismo , Fatores de Transcrição Forkhead/genética , Fatores de Transcrição Forkhead/metabolismo , Humanos , Imunossupressores/uso terapêutico , Lactente , Subunidade alfa de Receptor de Interleucina-15/metabolismo , Subunidade alfa de Receptor de Interleucina-2/antagonistas & inibidores , Subunidade beta de Receptor de Interleucina-2/genética , Subunidade beta de Receptor de Interleucina-2/metabolismo , Transplante de Fígado , Masculino , Pessoa de Meia-Idade , Receptores de Interleucina-7/metabolismo , Proteínas Recombinantes de Fusão/uso terapêutico , Estudos Retrospectivos , Linfócitos T Reguladores/imunologia , Adulto JovemRESUMO
The present report describes AIRE gene analysis in 25 children with autoimmune hepatitis type I or II. The heterozygous transversion c.961C > G (p.Ser278Arg) located in exon 7 was identified in 4 patients with autoimmune hepatitis type I, and mostly in those presenting with a positive family history for autoimmune diseases. In this subgroup of patients, the allelic frequency of this polymorphic variant was at least 3-fold higher than in healthy controls. These results suggest that heterozygous AIRE gene mutation may represent a genetic predisposition to childhood autoimmune hepatitis type I.
Assuntos
Predisposição Genética para Doença , Hepatite Autoimune/genética , Fatores de Transcrição/genética , Adolescente , Criança , Pré-Escolar , Éxons , Feminino , Frequência do Gene , Heterozigoto , Humanos , Mutação Puntual , Polimorfismo Genético , Análise de Sequência de DNA , Proteína AIRERESUMO
Transplant recipients are very susceptible to invasive aspergillosis, which increases mortality rate. Disseminated aspergillosis in the liver transplant recipient can affect virtually any organ and endocarditis is often lethal despite cardiac surgery and antifungal therapy. We report the case of a eight-month-old girl who presented with Aspergillus fumigatus endocarditis 18 days after liver transplantation that was successfully treated by a combination of antifungal drugs associated to a low dosage of immunosuppressive therapy.
Assuntos
Aspergilose/etiologia , Aspergillus fumigatus/metabolismo , Endocardite/etiologia , Endocardite/microbiologia , Transplante de Fígado/métodos , Administração Oral , Aspergilose/microbiologia , Ecocardiografia Doppler/métodos , Feminino , Transplante de Coração , Humanos , Imunossupressores/uso terapêutico , Lactente , Transplante de Fígado/efeitos adversos , Reação em Cadeia da Polimerase , Complicações Pós-Operatórias , Fatores de Tempo , Resultado do TratamentoRESUMO
We report on a 3-year-old Melanesian girl admitted for acute renal failure following subfulminant hepatitis A virus infection. While the child was slowly recovering from severe cytolytic hepatitis, she presented 8 weeks of protracted fever and major eosinophilia (30,000/microl); thereafter, acute renal failure (serum creatinine 295 micromol/l) occurred. Renal histology displayed diffuse eosinophilic infiltrate, with severe acute tubulointerstitial lesions associated with mild glomerular endocapillary proliferation and eosinophilic infiltrate, suggesting an immunoallergic mechanism. The child had received cefixime and cotrimoxazole 3 weeks prior to hospitalisation for the hepatitis A virus infection. The final diagnosis was of the syndrome drug reaction with eosinophilia and systemic symptoms or DRESS, induced by cefixime or cotrimoxazole and possibly triggered by the hepatitis A virus infection.