Exploring the potential of mapped soil properties, rhizobium inoculation, and phosphorus supplementation for predicting soybean yield in the savanna areas of Nigeria.

Rapid and accurate soybean yield prediction at an on-farm scale is important for ensuring sustainable yield increases and contributing to food security maintenance in Nigeria. We used multiple approaches to assess the benefits of rhizobium (Rh) inoculation and phosphorus (P) fertilization on soybean yield increase and profitability from large-scale conducted trials in the savanna areas of Nigeria [i.e., the Sudan Savanna (SS), Northern Guinea Savanna (NGS), and Southern Guinea Savanna (SGS)]. Soybean yield results from the established trials managed by farmers with four treatments (i.e., the control without inoculation and P fertilizer, Rh inoculation, P fertilizer, and Rh + P combination treatments) were predicted using mapped soil properties and weather variables in ensemble machine-learning techniques, specifically the conditional inference regression random forest (RF) model. Using the IMPACT model, scenario analyses were employed to simulate long-term adoption impacts on national soybean trade and currency. Our study found that yields of the Rh + P combination were consistently higher than the control in the three agroecological zones. Average yield increases were 128%, 111%, and 162% higher in the Rh + P combination compared to the control treatment in the SS, NGS, and SGS agroecological zones, respectively. The NGS agroecological zone showed a higher yield than SS and SGS. The highest training coefficient of determination (R2 = 0.75) for yield prediction was from the NGS dataset, and the lowest coefficient (R2 = 0.46) was from the SS samples. The results from the IMPACT model showed a reduction of 10% and 22% for the low (35% adoption scenario) and high (75% adoption scenario) soybean imports from 2029 in Nigeria, respectively. A significant reduction in soybean imports is feasible if the Rh + P inputs are large-scaled implemented at the on-farm field and massively adopted by farmers in Nigeria.

Thyme Oil Enhances the Inactivation of Salmonella enterica on Raw Chicken Breast Meat During Marination in Lemon Juice With Added Yucca schidigera Extract.

Enteric pathogens such as Salmonella enterica can survive in low pH conditions and pose a food safety threat during marinating of raw poultry meat. A study was conducted to investigate the effectiveness of thyme oil for killing S. enterica on raw chicken during marination in lemon juice containing yucca extract. Samples of raw chicken breast were inoculated with a five-serovar mixture of S. enterica (~108 CFU/mL) and immersed for 2, 4, 6, and 8 h in four lemon-based marinades at 22°C: lemon juice alone (L), L with added 0.5% yucca extract (L + Y), L + Y and 0.5% thyme oil (L + Y + 0.5% TO) and L + Y + 1.0% TO. The L and L + Y served as controls. Survivors were determined by surface plating chicken homogenates on xylose-lysine tergitol-4 (XLT4) agar and XLT4 agar overlaid with non-selective agar (TAL) and counting bacterial colonies after 48 h of incubation (35°C). Marinades containing Y and TO significantly reduced initial viable populations of S. enterica compared to control (L and L + Y) solutions (P < 0.05). Based on S. enterica survivors on TAL medium, the L and L + Y reduced initial populations by 1.12 and 1.42 Log CFU/sample, respectively, after 8 h whereas, Log reductions caused by L + Y + 0.5% TO and L + Y + 1.0% TO, respectively, were 2.62 and 3.91 (P < 0.05). Numbers of survivors were higher on TAL compared to XLT4 agar (P < 0.05); however, the extent of sub-lethal injury caused by the marinades was not statistically significant (P > 0.05). The death rate of S. enterica increased significantly (P < 0.05) in the marinades containing TO (0.5 or 1.0%) compared to control (L + Y). Based on these results, thyme oil has good potential to increase the antimicrobial efficacy of lemon juice marinade against Salmonella on raw chicken breast and enhance the microbial safety of this popular poultry product.

In-package decontamination of chicken breast using cold plasma technology: Microbial, quality and storage studies.

Atmospheric cold plasma (ACP) is a promising non-thermal technology for controlling food spoilage. In this study, ACP treatment at 100 kV for 1, 3 and 5 min was applied to chicken breast samples. Approximately 2 log CFU/g reduction in natural microflora of chicken was achieved within 5 min of treatment and 24 h of storage. The observed reduction was attributed to the reactive oxygen and nitrogen species in cold plasma. For shelf-life study, control and ACP treated samples (100 kV for 5 min) were analysed for the population of mesophiles, psychrotrophs and Enterobacteriaceae as well as sample colour and pH over a storage period of 24 days. On day 24, the population of mesophiles, psychrotrophs and Enterobacteriaceae in treated chicken was respectively 1.5, 1.4 and 0.5 log lower than the control. These results suggest that in-package ACP is an effective technology to extend the shelf-life of poultry products.