[Determination of the heparin content in basophilic granulocytes by cytophotometry]. / Opredeleneie soderzhaniia geparina v bazofil'nykh granulotsitakh s pomoshch'iu tsitofotometrii.

A method for estimating heparin content in basophilic leucocytes by means of cytophotometry of alcian blue--heparin complex is proposed. Its application for measuring heparin amounts in basophils of healthy donors and of patients with allergies is demonstrated.

[Inhibition of ADP-ribosylation by nerve growth factor in the pheochromocytoma PC-12 cell line]. / Ingibirovanie ADP-ribozilirovaniia pod deistviem faktora rosta nervov v vkletochnoi linii feokhromotsitomy PC-12.

The effect of nerve growth factor (NGF), purified to homogeneity from bovine seminal plasma using HPLC, on the level of endogenous ADP-ribosylation in pheochromocytoma PC-12 cell line was studied. NGF caused a 30% inhibition of ADP-ribosylation in the cellular homogenate, a 25% inhibition during serum-free cultivation, and a 50% inhibition in the presence of serum in the culture medium. NGF inhibited ADP-ribosylation of several proteins, including a protein with molecular weight of 40,000, probably of membrane origin. A possibility of the interrelation between NGF and cyclase system via receptor-dependent ADP-ribosylation of regulatory components of the adenylate cyclase was discussed.