Assuntos
Modelos Animais de Doenças , Infecções por Haemophilus/microbiologia , Haemophilus influenzae tipo b/patogenicidade , Animais , Dextranos/farmacologia , Hemoglobinas/farmacologia , Ferro/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Óleo Mineral/farmacologia , Muco , Tripsina/farmacologiaRESUMO
A solid phase immunoradiometric assay (IRMA) for quantification of bovine albumin fraction V (Bov.Alb.FV) in antileptospirosical Cuban vaccine vaxSpiral is described in the present work. Anti-Bov.Alb.FV IgG raised against rabbit purified by affinity chromatography was used as first antibody. Anti-rabbit IgG labeled by Chloromine-T reaction was used as a tracer and the method has demonstrated to be sensitive with high intra- and inter-assay reproducibility. Eight lots of vaccinal antigens were evaluated and in all of the cases, the bovine albumin fraction V concentration was lower than 1 microgram/mL, as the World Health Organization (WHO) establishes. This IRMA is a simple and sensitive assay and could be used as control method for all human vaccines that use Bov.Alb.FV in their production process, even cellular vaccines.