RESUMO
INTRODUCTION: The function, peripheral blood expression, and physiologic importance of IL-17 is not well established. Detection of IL-17 in sera and plasma samples from patients with pre-eclampsia has been reported with inconsistent results. To establish the l levels of the IL-17 at peripheral level, we studied prospectively a cohort of 13 healthy pregnant women. OBJECTIVE: To evaluate the changes in serum levels of IL-17 in healthy pregnant women in a prospective cohort. MATERIAL AND METHODS: Thirteen healthy pregnant women were prospectively followed to evaluate serum levels of IL-17. Each patient was evaluated at each trimester. IL-17 levels were measured by ELISA. The statistical analysis was done using repeated measures anova and Bonferroni's multiple comparison test. RESULTS: IL-17 levels were significantly increased from first trimester with a mean of 14.61 up to 31.78 pg/mL at third trimester (P < 0.05), but when detectable, they were almost identical range in all trimesters. CONCLUSIONS: We propose that IL-17 levels in healthy women are present with very similar range levels during the whole pregnancy but the average is increased during the third trimester maybe as a part of the complex network of cytokines as a result of implantation, fetal development, and labor process itself.
Assuntos
Interleucina-17/sangue , Terceiro Trimestre da Gravidez/sangue , Adulto , Feminino , Humanos , Gravidez , Primeiro Trimestre da Gravidez/sangue , Segundo Trimestre da Gravidez/sangue , Nascimento a Termo , Regulação para Cima , Adulto JovemRESUMO
Phosphorylation is the most important post-translational event at a cellular level that is regulated by protein kinases. MAPK is a key player in the important cellular signaling pathway. It has been hypothesized that phosphorylation might have a role in the induction of break tolerance against some autoantigens such as SRP72. The aim of this study was to explore the pathways of phosphorylation and overexpression of the SRP72 polypeptide, using an in vitro model of Jurkat cells stimulated by recombinant human (rh)IL-1ß in the presence of MAPK inhibitors. We used Jurkat cells as a substrate stimulated with rhIL-1ß in the presence of MAPK inhibitors at different concentrations in a time course in vitro experiment by immunoprecipitation, immunoprecipitation-Western blotting, and real time PCR. Our results showed that rhIL-1ß causes up-regulation of protein expression and phosphorylation of SRP72 in Jurkat cells. Inhibitors of the MAPK pathway ERK1/2 or p38α/ß down-regulate the expression of SRP72 autoantigen in Jurkat cells stimulated by rhIL-1ß. Our results highlight the importance of studying the pathways of activation and overexpression of autoantigens. It will be necessary to perform careful research on various kinases pathways, including MAPK in dermatomyositis and other rheumatic diseases, to help to explain the routes of activation and inhibition of autoantigens. The understanding of this process may help to develop new therapies to prevent and control the loss of tolerance toward own normal proteins.