RESUMO
C-band polymorphisms in peripheral blood lymphocytes of 62 patients (33 with colon adenomas and 29 with colon carcinomas) were studied. A significant difference in the frequency of heterochromatic variants in chromosomes #1 in both colon adenoma (56%) and carcinoma (67%) with respect to controls (18%) was observed (p less than 0.001). The heterochromatic variants preferentially involved in both pathologies were inv(1), 1qh-, and inv(9), compared with controls. No differences were found between colon adenomas and carcinomas. We suggest that 1qh- and inv(1) variants are important heterochromatic changes in neoplasia.
Assuntos
Adenoma/genética , Carcinoma/genética , Neoplasias do Colo/genética , Marcadores Genéticos , Polimorfismo Genético , Bandeamento Cromossômico , Cromossomos Humanos Par 1 , Cromossomos Humanos Par 16 , Cromossomos Humanos Par 9 , Humanos , CariotipagemRESUMO
A study of heterochromatic regions in chromosomes #1, #9, and #16 was performed on lymphocytes of peripheral blood from 55 normal individuals and 50 patients with non-Hodgkin's lymphoma (NHL). Heteromorphism was present in 90% of the NHL patients, compared with 44% in normal individuals (p less than 0.001). An increase of inv(1), 1qh-, and 9qh-variants was observed in malignant lymphoma patients with respect to controls.
Assuntos
Marcadores Genéticos , Heterocromatina/genética , Linfoma não Hodgkin/genética , Polimorfismo Genético , Adolescente , Adulto , Idoso , Criança , Cromossomos Humanos Par 1 , Cromossomos Humanos Par 16 , Cromossomos Humanos Par 9 , Humanos , Pessoa de Meia-IdadeRESUMO
The cell cycle kinetics in peripheral blood lymphocytes from 30 patients with hematologic diseases, including non-Hodgkin lymphomas [10], acute nonlymphoblastic leukemias [10], and myelodysplastic syndromes [10] were studied. Thirty normal healthy subjects formed the control group. Non-Hodgkin lymphoma patients showed an elongation of the cell cycle time (43% of metaphases in the first cycle), whereas leukemic patients presented a shortening of the cell cycle progression with 46% of cells in the third division. Myelodysplastic syndromes showed most of the metaphases (55%) in the second cycle.
Assuntos
Ciclo Celular , Doenças Hematológicas/patologia , Doença Aguda , Humanos , Leucemia/patologia , Linfoma não Hodgkin/patologia , Índice Mitótico , Síndromes Mielodisplásicas/patologiaRESUMO
The incidence of heterochromatic variants was assessed in 26 patients with multiple myeloma (MM) and 55 control individuals. An enhanced frequency of heteromorphism was present in 92% of the MM population compared with 44% of the control group (p less than 0.001). Significant differences with regard to controls were observed in chromosome pairs #1, #9, and #16 due to 1qh-, inv(1),inv(9) and 16qh- variants. We suggest that MM would present an intermediate heterochromatic behavior between hematologic diseases and solid tumors.
Assuntos
Aberrações Cromossômicas , Heterocromatina/genética , Mieloma Múltiplo/genética , Polimorfismo Genético , Cromossomos Humanos Par 1 , Cromossomos Humanos Par 16 , Cromossomos Humanos Par 9 , Marcadores Genéticos , HumanosAssuntos
Desinfetantes/efeitos adversos , Linfócitos/efeitos dos fármacos , Acetato de Fenilmercúrio/efeitos adversos , Compostos de Fenilmercúrio/efeitos adversos , Troca de Cromátide Irmã/efeitos dos fármacos , Exposição Ambiental , Humanos , Lactente , Cuidado do Lactente , Recém-Nascido , Linfócitos/ultraestrutura , Fatores de TempoRESUMO
This report describes the chromosomal damage produced by 2-(4'-thiazolyl)benzimidazole or thiabendazole (TBZ) evaluated by "in vivo" and "in vitro" cytogenetic tests. The doses assayed in adult mice by the sister-chromatid exchange (SCE) and micronucleus tests were: 50, 100 and 200 mg/kg body weight; these are within the range of those used in human antihelminthic treatments. SCE frequency was increased only in the last dose (p less than 0.05). A significant increase of micronucleated cells was shown in the 3 doses assayed (p less than 0.001). A marked increase in abnormal anaphase-telophase cells was only detected with the two highest concentrations assayed (0.60-0.24 microgram/ml) p less than 0.01 and p less than 0.05 respectively. The observed genotoxic effects of this compound indicate that TBZ itself is a mutagenic agent.
Assuntos
Núcleo Celular/efeitos dos fármacos , Troca de Cromátide Irmã/efeitos dos fármacos , Tiabendazol/farmacologia , Anáfase/efeitos dos fármacos , Animais , Linhagem Celular , Núcleo Celular/ultraestrutura , Cricetinae , Feminino , Fibroblastos/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos , Testes de Mutagenicidade , Telófase/efeitos dos fármacos , Tiabendazol/administração & dosagemRESUMO
A study of the heteromorphism of chromosomes #1, #9, and #16 was performed in the cells of 55 normal subjects and in those of 40 preleukemic patients including those with refractory anemia (RA) and sideroblastic anemia (SA), classified on the basis of the FAB nomenclature. Heteromorphism was present in 85% of the preleukemic patients, compared with 44% in normal controls (p less than 0.01). The patient population presented an increased incidence of C-band size variants in chromosome #1 (1qh+ and 1qh-), while chromosomes #9 and #16 showed no difference, compared with the findings in the control group.
Assuntos
Cromossomos Humanos 1-3 , Cromossomos Humanos 16-18 , Cromossomos Humanos 6-12 e X , Variação Genética , Heterocromatina/ultraestrutura , Pré-Leucemia/genética , Anemia Aplástica/genética , Anemia Sideroblástica/genética , Bandeamento Cromossômico , Humanos , Linfócitos/citologia , Neoplasias/genéticaRESUMO
C-banding studies of the heteromorphism of chromosomes #1, #9, and #16 were performed in 120 leukemic patients: 56 with chronic myelocytic leukemia (CML), 45 with acute lymphoblastic leukemia (ALL), and 19 with acute nonlymphoblastic leukemia (ANLL). No differences were found among patients and controls with regard to sex. Our data showed a significant increase of polymorphism in chromosome #1 in the three neoplastic groups; the heterochromatic variant preferentially involved 1qh-, whereas there were no significant differences in heteromorphism in chromosomes #9 and #16.
Assuntos
Variação Genética , Heterocromatina/fisiologia , Leucemia/genética , Doença Aguda , Bandeamento Cromossômico , Cromossomos Humanos 1-3 , Cromossomos Humanos 16-18 , Cromossomos Humanos 6-12 e X , Humanos , Leucemia Linfoide/genética , Leucemia Mieloide/genética , Fatores SexuaisRESUMO
The genotoxic effect of AMSA, an anti-tumor agent, was evaluated using the micronucleus and anaphase-telophase tests. The doses assayed by the in vivo micronucleus test were 1.5, 3 and 6 mg/kg: they are within the range of those used in clinical trials. A significant increase of micronucleated cells (P less than 0.01) was observed in the three assayed doses, with a linear dose response (r 0.98). In the in vitro test, 3 drug concentrations, i.e. 10, 1 and 0.1 microgram/ml, were analyzed with the 2 higher doses. AMSA showed a marked inhibition of cellular replication, but with 0.1 microgram/ml it was possible to determine an increase (P less than 0.01) in aberrations in anaphase-telophase cells. Both studies clearly demonstrate the clastogenic effect of the drug, which should be taken into account when considering its carcinogenic risk.
Assuntos
Aminoacridinas/toxicidade , Núcleo Celular/efeitos dos fármacos , Mitose/efeitos dos fármacos , Amsacrina , Anáfase/efeitos dos fármacos , Animais , Medula Óssea/efeitos dos fármacos , Células Cultivadas , Cricetinae , Feminino , Masculino , Camundongos , Testes de Mutagenicidade , Ovário , Telófase/efeitos dos fármacosRESUMO
The karyotype of the Argentine "Cai" (Cebus apella) is studied by different banding techniques (C and G). The analysis takes into account the results obtained from 15 specimens according to their geographic habitat. Comparative studies between phenotypic and karyotypic features suggest a phenotypic sub-speciation instead of a chromosomal. This proposition is supported by the observed karyologic stability. According to this report the genus Cebus and the species apella remains controversial from the systematic point of view.
Assuntos
Cebidae/genética , Cebus/genética , Animais , Cebus/classificação , Bandeamento Cromossômico , Feminino , Cariotipagem , Masculino , Fenótipo , Especificidade da EspécieRESUMO
Se realizo el estudio citogenetico, aplicando la prueba de ICH, para valorar el efecto del tiabendazol, farmaco ampliamente utilizado como antiparasitario. Se estudiaron 4 monos de la especie Saimiri sciureus, provenientes de Santa Cruz de la Sierra (Bolivia), tratados con dosis que corresponden a la mitad de la dosis terapeutica (40 mg/kg/dia durante 5 dias) y 3 ejemplares,virgenes de tratamiento, de igual origen geografico, considerados controles de los anteriores. La media de ICH de los ejemplares tratados fue de 8.7 + o - 0.3, mientras que la media de ICH de los ejemplares controles fue de 5.6 + o - 0.3. La valoracion estadistica demonstro la existencia de diferencias altamente significativas (p < 0.01) indicando un efecto clastogenico de la droga o de sus derivados. Si la droga es clastogenica debe tenerse en cuenta que existe un riesgo potencial para la poblacion tratada con dicho producto quimico
Assuntos
Troca de Cromátide Irmã , TiabendazolRESUMO
Se realizo el estudio citogenetico, aplicando la prueba de ICH, para valorar el efecto del tiabendazol, farmaco ampliamente utilizado como antiparasitario. Se estudiaron 4 monos de la especie Saimiri sciureus, provenientes de Santa Cruz de la Sierra (Bolivia), tratados con dosis que corresponden a la mitad de la dosis terapeutica (40 mg/kg/dia durante 5 dias) y 3 ejemplares,virgenes de tratamiento, de igual origen geografico, considerados controles de los anteriores. La media de ICH de los ejemplares tratados fue de 8.7 + o - 0.3, mientras que la media de ICH de los ejemplares controles fue de 5.6 + o - 0.3. La valoracion estadistica demonstro la existencia de diferencias altamente significativas (p < 0.01) indicando un efecto clastogenico de la droga o de sus derivados. Si la droga es clastogenica debe tenerse en cuenta que existe un riesgo potencial para la poblacion tratada con dicho producto quimico
