Immobilization of metribuzin degrading bacterial consortium MB3R on biochar enhances bioremediation of potato vegetated soil and restores bacterial community structure.

Metribuzin (MB) is a triazinone herbicide used for the eradication of weeds in agriculture. Presence of its residues in agricultural soil can potentially harm the establishment of subsequent crops and structure of soil microbial populations. In this study, remediation potential of an MB degrading bacterial consortium MB3R immobilized on biochar was evaluated in potato vegetated soil. In potato vegetated soil augmented with MB3R alone and MB3R immobilized on biochar, 82 and 96% MB degradation was recorded respectively as compared to only 29.3% in un-augmented soil. Kinetic parameters revealed that MB3R immobilized biochar is highly proficient as indicated by significant increase in the rate of biodegradation and decrease in half-life of MB. Enhanced plant growth was observed when augmented with bacterial consortium either alone or immobilized on biochar. Presence of herbicide negatively affected the soil bacterial community structure. However, MB3R immobilized on biochar proved to be helpful for restoration of soil bacterial community structure affected by MB. This is the very first report that reveals improved remediation of contaminated soil and restoration of soil bacterial populations by use of the MB degrading bacterial consortium immobilized on biochar.

Optimizing the metribuzin degrading potential of a novel bacterial consortium based on Taguchi design of experiment.

Metribuzin (MB) is used for control of weeds in crops like potato, maize and sugarcane. Its extensive and unjudicial use has resulted in various environmental issues; hence it is very critical to remediate this herbicide at the respective point source. Plant associated, MB degrading bacterial strains, Rhodococcus rhodochrous sp. AQ1, Bacillus tequilensis sp. AQ2, Bacillus aryabhattai sp. AQ3 and Bacillus safensis sp. AQ4 were isolated, and a consortium MB3R was developed. For degradation of MB by the consortium MB3R, various parameters i.e., pH, temperature, inoculum density and pesticide concentration were optimized by using Taguchi design of experiment (DOE). MB degradation was dependent upon all the four factors. The contribution of each factor on MB degradation was according to the order: temperature > inoculum density > pH > pesticide concentration. Fitness of Taguchi DOE in forecasting the optimum response, was confirmed experimentally by using optimized levels of the four factors i.e., pH 7.0, temperature 30 °C, pesticide concentration 45 mg l-1 and an inoculum density of 5.0 × 10 5 CFU ml-1 whereby 98.63% MB degradation was observed. Appearance and subsequent degradation of three MB metabolites, desamino-metribuzin (DA), diketo-metribuzin (DK) and desamino-diketo-metribuzin (DADK) during biodegradation by the consortium was observed.