RESUMO
The classification of white blood cell neutrophils into band neutrophils (bands) and segmented neutrophils (segs) is a subproblem of the white blood cell differential count. This classification problem is not well defined for at least two reasons: (a) there are no unique quantitative definitions for bands and segs and (b) existing definitions use the shape of the nucleus as the only discriminating criterion. When cells are classified on a slide, decisions are made from the two-dimensional views of these three-dimensional cells. A problem arises because the exact shape of the nucleus becomes indeterminate when the nucleus overlaps so that the filament is hidden. To assess the importance of this problem, this paper quantitates the classification errors due to overlapped nuclei (ON). The results indicate that, using only neutrophils without ON, the classification accuracy is 89%. For neutrophils with ON, the classification accuracy is 65%. This suggests a classification strategy of first classifying neutrophils into three categories: (a) bands without ON, (b) segs without ON and (c) neutrophils with ON. Category III can then be further classified into segs and bands by other stretegies.
