RESUMO
The 3'-pyrophosphate derivative of CoA was synthesized by using the excreted 5'-to-3' pyrophosphoryl-transferring enzyme from Streptomyces adephospholyticus and ATP as donor and dephospho-CoA as acceptor. Cofactor activity of this new coenzyme A derivative was tested with Clostridium kluyveri phosphotransacetylase and hog heart succinic thiokinase. With the phosphotransacetylase, 3'-pyrophospho-CoA was found to be twice as active as CoA whereas dephospho-CoA was inactive. However, succinic thiokinase utilized all three types of CoA equally well. Adenosine 5'-monophosphate 3'-pyrophosphate also was synthesized and used as an analog of adenosine 5'-monophosphate 3'-monophosphate in the dog liver's sulfotransferase-catalyzed sulfate transfer from p-nitrophenyl sulfate to phenol. In contrast to the pyrophospho derivative of coenzyme A, adenosine 5'-monophosphate 3'-pyrophosphate was inactive as a cofactor.
Assuntos
Coenzima A/análogos & derivados , Animais , Coenzima A/metabolismo , Cinética , Fosfato Acetiltransferase/metabolismo , Fosforilação , Relação Estrutura-Atividade , Succinato-CoA Ligases/metabolismo , SuínosAssuntos
Escherichia coli/enzimologia , Galactosidases/biossíntese , Polifosfatos/farmacologia , Nucleosídeos de Purina/farmacologia , Nucleosídeos de Pirimidina/farmacologia , beta-Galactosidase/biossíntese , AMP Cíclico/farmacologia , GMP Cíclico/farmacologia , Indução Enzimática , Lítio/farmacologia , Magnésio/farmacologiaAssuntos
Nucleotídeos de Desoxiadenina/síntese química , Nucleotídeos de Desoxiguanina/síntese química , Difosfotransferases , Fosfotransferases , Streptomyces/enzimologia , Nucleotídeos de Timina/síntese química , Fenômenos Químicos , Química , Cobalto , Concentração de Íons de Hidrogênio , Nucleotídeos , Fosforilação , TemperaturaRESUMO
All the eight 5'-di(tri)-3'-diphosphates of four common ribonucleosides were prepared by the enzymic pyrophosphoryl transfer catalysed by Streptomyces adephospholyticus ATP:nucleotide pyrophosphokinase (E.C.2.7.6.4) form dATP to the respective 5'-phosphates, and their effects on the translation of mRNAs by a wheat germ system in vitro were studied. (p) ppPupp decreased the total 14C-leucine incorporation directed by a rat liver mRNA whereas (p) ppPypp did not. With a silkworm pupa ovary mRNA, distinctly reverase results were obtained. Gel electrophoretic profiles of the translation products disclosed the mRNA species-specific stimulatory or inhibitory effects for each of the polyphosphates tested.
Assuntos
Nucleotídeos de Adenina/farmacologia , Nucleotídeos de Citosina/farmacologia , Difosfotransferases , Nucleotídeos de Guanina/farmacologia , Guanosina Tetrafosfato/farmacologia , Fosfotransferases/metabolismo , Biossíntese de Proteínas/efeitos dos fármacos , RNA Mensageiro/metabolismo , Nucleotídeos de Uracila/farmacologia , Animais , Cinética , Fígado/metabolismo , Nucleotídeos/metabolismo , Plantas/metabolismo , Ratos , Streptomyces/enzimologia , Triticum/metabolismoAssuntos
Oxirredutases do Álcool/metabolismo , Flavina-Adenina Dinucleotídeo/metabolismo , NAD/metabolismo , D-Aminoácido Oxidase/metabolismo , Flavina-Adenina Dinucleotídeo/análogos & derivados , NAD/análogos & derivados , Nucleotídeos , Fosfotransferases/metabolismo , Streptomyces/enzimologia , Relação Estrutura-AtividadeRESUMO
Both silkworm nuclease and nuclease O of Aspergillus oryzae mycelia hydrolyse DNA endolytically to di- and trinucleotides terminating in 5'-phosphate. These oligonucleotides were fractionated first be DEAE-cellulose chromatography with 7 M urea into the respective isoplithic groups and then analysed for the composition and isomerism: each group was labeled 5'-terminally by the [gamma-32P]ATP-polynucleotide kinase reaction and then electrophoresed monodimensionally for the dinucleotides and two-dimensionally for the di- and trinucleotides mixtures, respectively, followed by elution and digestion with snake venom exonuclease. Both nucleases gave rather similar simple maps in which all sixteen dinucleotides and almost all the possible trinucleotides were identified, indicating their random mode of actions.
