RESUMO
Hypoxia is a wide spread phenomena throughout the world's aquatic systems. This paper investigates the functional state of scorpion fish red blood cells under acute hypoxia between 0.57-8.17 mg O2/l (in vitro) by assessing of the fluorescence of Rhodamine 123 (R123) and Fluorescein diacetate (FDA). Acute hypoxia induced a significant increase in the fluorescence of both fluorochromes (R123 - 12-60, FDA - 30-184%). This reaction is based on a probable decrease in erythrocyte membrane permeability. The reduction of functional ionic channels in red blood cells delays FDA in the cytoplasm and lowers the energy costs required for transmembrane cationic gradient maintenance, which reflects an intensification of R123 fluorescence.
Assuntos
Eritroblastos/efeitos dos fármacos , Membrana Eritrocítica/efeitos dos fármacos , Oxigênio/farmacologia , Animais , Hipóxia Celular , Permeabilidade da Membrana Celular , Eritroblastos/citologia , Eritroblastos/metabolismo , Membrana Eritrocítica/metabolismo , Citometria de Fluxo , Fluoresceínas/química , Fluoresceínas/metabolismo , Fluorescência , Corantes Fluorescentes/química , Corantes Fluorescentes/metabolismo , Transporte de Íons , Perciformes , Cultura Primária de Células , Rodamina 123/química , Rodamina 123/metabolismoRESUMO
Cyanophages of the family Myoviridae were studied in Lake Hovsgol based on the analysis of g20 gene fragments. The analysis revealed the diversity of g20 cyanomyovirus sequences in Lake Hovsgol. It was found a great similarity of genes from the Lake Hovsgol and Lake Baikal. Distribution of closely related virus strains in these water bodies is attributed to close geographical location, direct water connection and similar hydrochemical parameters of the lakes.
