RESUMO
The neutrophil-dominated inflammation of the lung in cystic fibrosis (CF) has traditionally been seen as a physiological response to continuous opportunistic infection. Recent studies suggest, however, that regulation of the inflammatory response itself may be altered in CF. Neutrophil migration from the bloodstream involves alterations in surface expression of the adhesion molecules L-selectin and Mac-1 (CD11b/CD18). The aim of this study was to assess neutrophil adhesion molecule expression and responsiveness in CF. Neutrophils from chronic (n = 16) and acutely infected (n = 13) CF patients and 15 normal control subjects were directly assessed by Fluorescence-activated cell sorter (FACS) analysis for surface expression of L-selectin and CD11b before and after stimulation with interleukin 8 (IL-8) or f-Met-Leu-Phe (fMLP). Neutrophils from stable (n = 5) and acutely infected (n = 5) non-CF bronchiectasis patients were also assessed. Surface upregulation of CD11b was similar in all groups. Basal levels of L-selectin were also comparable among all groups, however, when stimulated, neutrophils from both stable and acutely infected CF patients shed significantly less L-selectin than those from control subjects (p < 0.05 and p < 0.01, respectively). This decreased responsiveness was not observed in either stable or acutely infected non-CF bronchiectasis patients. These results add to the accumulating evidence suggestive of a defective inflammatory response in CF.
Assuntos
Fibrose Cística/imunologia , Selectina L/imunologia , Antígeno de Macrófago 1/imunologia , Neutrófilos/imunologia , Doença Aguda , Adolescente , Adulto , Idoso , Bronquiectasia/imunologia , Antígenos CD11/genética , Antígenos CD11/imunologia , Antígenos CD18/genética , Antígenos CD18/imunologia , Movimento Celular , Separação Celular , Quimiotaxia de Leucócito/imunologia , Doença Crônica , Feminino , Citometria de Fluxo , Regulação da Expressão Gênica , Humanos , Interleucina-8/farmacologia , Selectina L/genética , Antígeno de Macrófago 1/genética , Masculino , Pessoa de Meia-Idade , N-Formilmetionina Leucil-Fenilalanina/farmacologia , Ativação de Neutrófilo/imunologia , Infecções por Pseudomonas/imunologia , Pseudomonas aeruginosa/imunologia , Regulação para CimaRESUMO
A study has been made of the variations in the results of the lepromin test in cases the neural type, demonstrated by repeated testing of the same cases in different circumstances There apper to be at least five possible factores which, acting singly or in combination, may influence the result of the test. These different factors are: variations in clinical activity, variations in bacteriological findings, variations in the time year at which the test is done, deterioration of the lepromin on keeping, and the use of different lots of lepromin Our stdy has shown that the two most important factors are the variations in clinical activity and tima of the yaer at which the test is done
