RESUMO
Fluoride has toxic potential particularly for teeth, bones, and kidney. This study was aimed to investigate the NaF exposure effects on the growth of ameloblasts and kidney proximal tubular cells. Adult male healthy rats were used as experiment models, divided into control and NaF-induced groups. The expression of amelogenin, Bcl-2, and caspase-3 were significantly different in the control and NaF-induced group (P < 0.05). There was no correlation among these proteins in the control group but significant correlation in the NaF-induced group (r = 0.694). There was a significant correlation in proximal tubular cells, as seen from the increase of caspase-3 in the NaF-induced group (r = 0.715).
Assuntos
Ameloblastos/citologia , Túbulos Renais Proximais/citologia , Fluoreto de Sódio/farmacologia , Ameloblastos/efeitos dos fármacos , Ameloblastos/metabolismo , Animais , Caspase 3/metabolismo , Proliferação de Células/efeitos dos fármacos , Células Epiteliais/metabolismo , Imuno-Histoquímica , Masculino , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , RatosRESUMO
This study reports the molecular characterization of ovalocytosis in Lombok Island, Indonesia. The analysis of genomic DNA by polymerase chain reaction shows that all 21 ovalocytotic individuals have two amplified products of different size from a region encompassing exon 11 of the band 3 gene. The sequence of the larger product matched perfectly with that of normal individuals. In the sequence of the smaller product, 27 nucleotides within exon 11 were deleted. The heterozygous presence of the deletion identified in other parts of Southeast Asia was confirmed in patients with ovalocytosis in an isolated island of eastern Indonesia.
Assuntos
Proteína 1 de Troca de Ânion do Eritrócito/genética , Sequência de Bases , Eliptocitose Hereditária/genética , Nucleotídeos/genética , Deleção de Sequência/genética , Proteína 1 de Troca de Ânion do Eritrócito/fisiologia , Eliptocitose Hereditária/sangue , Eliptocitose Hereditária/fisiopatologia , Éxons/genética , Éxons/fisiologia , Humanos , Indonésia , Dados de Sequência Molecular , Nucleotídeos/fisiologiaRESUMO
The concentrations of serum retinol-binding protein (RBP), prealbumin (PA) and vitamin A of Indonesian neonates were compared with those of Japanese neonates. The mean serum concentrations of both PA and vitamin A did not differ significantly in Indonesian and Japanese neonates. Indonesian neonates had significantly higher serum RBP (2.75 +/- 0.87 mg/dl, mean +/- SD) than Japanese neonates (1.65 +/- 0.55 mg/dl, p less than 0.01). The molar ratio of vitamin A:RBP in Indonesian infants was significantly lower than in Japanese infants (p less than 0.02), and this in turn is indicative of increased concentrations of retinol-free RBP in the plasma of Indonesian neonates. The results suggest that RBP metabolism in Indonesian neonates differs from that in Japanese neonates.
