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J Mater Sci Mater Med ; 32(2): 19, 2021 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-33576907

RESUMO

Our study aimed at development of Silver, Iron and Gold nanoparticles of Lycopene isolated from tomato by using green synthesis technique and to evaluate its anticancer potential against colorectal and cervical cancer. Lycopene was extracted by benzene extraction method and the silver, iron and gold nanoparticles were developed by green synthesis method. 1% aqueous extract of isolated Lycopene was mixed with 1% solutions of AgNO3, FeCl3 and HAuCl4 solutions and incubated at ambient temperature for 3-4 h separately and observed for the color change which is an indicative of formation of the nanoparticles. The prepared nanoparticles were characterized by FTIR, SEM, XRD analysis and evaluated for their antimicrobial potential. The cytotoxicity studies were carried out by in vitro assay like MTT, SRB and Tryphan blue method against Colo 320 DM, HT 29, and Hella. SEM showed nanosized particles of 50-100 nm range, whereas no antimicrobial activity was exhibited by the prepared nanoparticles. In MTT assay the LyAgNP showed maximum 41.41 ± 0.4124% inhibition against COLO320DM, whereas LyGNP exhibited 41.47 ± 0.4469% inhibition against HT 29 and LyAgNP showed 40.9 ± 0.6908% inhibition against Hella cells. In SRB assay LyAgNP showed maximum 82.68 ± 1.1798% inhibition against COLO320DM, whereas LyGNP exhibited maximum 91.21 ± 0.2372% inhibition against HT29 and 87.98 ± 0.5878% inhibition against Hella cells. In tryphan blue assay against COLO320DM, HT29 and Hella cells, the maximum inhibition exhibited by the prepared nanoparticles were observed as LyGNP 83.45 ± 0.4694%, LyAgNP 88.05 ± 0.1870% and LyAgNP65.47 ± 0.4766%. We conclude that the developed nanoparticles of Lycopene exhibited potential anticancer activity against Colorectal and cervical cancer cell as compared with pure Lycopene.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Licopeno/farmacologia , Nanopartículas Metálicas/química , Antibacterianos/farmacologia , Antineoplásicos Fitogênicos/administração & dosagem , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Neoplasias Colorretais/tratamento farmacológico , Feminino , Ouro/química , Química Verde , Células HT29 , Células HeLa , Humanos , Ferro/química , Licopeno/administração & dosagem , Licopeno/isolamento & purificação , Solanum lycopersicum/química , Nanopartículas Metálicas/administração & dosagem , Nanopartículas Metálicas/ultraestrutura , Microscopia Eletrônica de Varredura , Nanotecnologia , Prata/química , Espectroscopia de Infravermelho com Transformada de Fourier , Neoplasias do Colo do Útero/tratamento farmacológico , Difração de Raios X
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