Ecological assessment of the career maintenance needs of employees with diabetes.

The article presents four case studies of employees with diabetes. The authors examine participants' post-employment accommodation needs in four areas: worksite accessibility; performance of essential job functions; job mastery; and job satisfaction. Data were collected via the Work Experience Survey, an established career maintenance needs assessment tool.

Diabetes mellitus: medical aspects and rehabilitation implications.

This article examines the etiologies, incidence and prevalence of diabetes mellitus - a chronic, systemic disease of the endocrine system. It presents current information for rehabilitation professionals concerning diagnosis, treatment, and physiological effects of this, most common of all endocrine disorders.

The role of the rehabilitation placement professional in the ADA era.

Rehabilitation placement professionals have a vital role in helping American corporations develop recruiting, hiring, and accommodation practices that are compatible with the civil rights protections in the Americans with Disabilities Act (ADA). To perform this consulting function, they must have current information on: (a) provisions and protections in Title I of the ADA; (b) the benefits of hiring people with disabilities; (c) the cost-effectiveness of reasonable accommodations; and (d) collaborative employer/employee approaches to job accommodations.

Employing people with disabilities.

By July 26, 1994, employers with 15 or more employees must comply with the Americans with Disabilities Act. By understanding the ADA regulations, you can recruit qualified, dedicated employees from a virtually untapped segment of the population.

Industrial emissions of 1,3-butadiene.

Sources of industrial emissions of 1,3-butadiene are discussed both by process (production, consumers) and type (equipment leaks, point sources). Quantification of the emissions are presented, as reported by the U.S. Environmental Protection Agency in 1986. The reported emissions attributed to equipment leaks (also known as fugitive emissions) range from about 50 to 95% of the total, depending on the specific production process used. The methods by which these emissions were estimated are discussed, with particular emphasis on the fugitive sources. Industry studies to better quantify the fugitive emissions are described.

Mung bean nuclease cleaves Plasmodium genomic DNA at sites before and after genes.

Mung bean nuclease was found to cut the genomic DNA of the malaria parasite Plasmodium at positions before and after genes but not within gene-coding regions. This cleavage, which had nearly the preciseness of a restriction nuclease, required controlled conditions in the presence of formamide. Southern blot analysis showed that the coding areas for Plasmodium actin, circumsporozoite protein, histidine-rich protein, ribosomal RNA's, and tubulin are each cleaved from genomic DNA to yield a single major band on an agarose gel. DNA sequence data on several clones of mung bean nuclease cleavage products containing the gene for the circumsporozoite protein of Plasmodium falciparum confirmed that cleavage sites are before and after genes. Recognition and cleavage of DNA did not seem to be related to any primary sequence but may be related to structural features of the DNA duplex that demarcate genes. Mung bean nuclease-cleaved DNA could be inserted directly into a lambda expression vector, yielding a representative but small gene bank of intact gene fragments.

Differentiation of schistosomes by species, strain, and sex by using cloned DNA markers.

We have detected species, strain, and sex-specific genetic markers for the genus Schistosoma by Southern blot analysis of its DNA using cloned DNA segments of the Schistosoma mansoni ribosomal gene as probes. Restriction analysis of DNA from eight different strains of S. mansoni, from Africa and the Caribbean, revealed that the predominant or major DNA fragment containing the ribosomal gene unit was the same in each but that low copy number or minor fragments containing the gene varied. It was shown that the detection of these minor fragments could serve as the basis for both strain differentiation and the analysis of individual differences within a strain. Analysis of the parents and progeny of a genetic cross revealed sex-linked markers and suggested that these markers are inherited in a Mendelian fashion. DNAs from the species Schistosoma haematobium and Schistosoma japonicum were also analyzed. Differences in the length of the major repeating unit of the ribosomal gene served to distinguish each species. Furthermore, an array of minor bands was detected in each species, suggesting that strains of S. haematobium and S. japonicum could be differentiated in the same manner as S. mansoni strains.