Human health risk surveillance of polychlorinated biphenyls in bovine milk from alluvial plain of Punjab, Pakistan.

Punjab is the leading province of Pakistan in the production of bovine milk and its consumption. Rapid industrialization, high energy demand, and the production of waste have increased the risk of polychlorinated biphenyls (PCBs) toxicity in the environment. This research work was designed to assess human dietary exposure of ∑PCBs17 congeners through ingestion of buffalo and cow's milk from eight main districts of Punjab, Pakistan. The average concentrations of ∑DL-PCBs (8.74 ng g-1 and 14.60 ng g-1) and ∑I-PCBs (11.54 ng g-1 and 18.68 ng g-1) in buffalo and cow milk samples were analyzed, respectively. The PCB-156 was predominantly high congener found in both buffalo (2.84 ng g-1) and cow milk (2.86 ng g-1). It was found that the highest PCBs in bovine milk samples were observed in close vicinities of urban and industrial areas. The estimated daily consumptions of DL-PCBs and I-PCBs, from buffalo and cow milk, were below the acceptable daily intake for both adults and children. Moreover, hazard quotients (HQ) of the ∑PCBs17 congener value were less than 1.0 in adults and greater in the case of children reflecting the high chances of cancer. Furthermore, comprehensive monitoring for childhood cancer is recommended to establish the relationship in future studies.

Novel strategies and advancement in reducing heavy metals from the contaminated environment.

The most contemporary ecological issues are the dumping of unprocessed factories' effluent. As a result, there is an increasing demand for creative, practical, environmentally acceptable, and inexpensive methodologies to remediate inorganic metals (Hg, Cr, Pb, and Cd) liquidated into the atmosphere, protecting ecosystems. Latest innovations in biological metals have driven natural treatment as a viable substitute for traditional approaches in this area. To eliminate pesticide remains from soil/water sites, technologies such as oxidation, burning, adsorption, and microbial degradation have been established. Bioremediation is a more cost-effective and ecologically responsible means of removing heavy metals than conventional alternatives. As a result, microorganisms have emerged as a necessary component of methyl breakdown and detoxification via metabolic reactions and hereditary characteristics. The utmost operative variant for confiscating substantial metals commencing contaminated soil was A. niger, which had a maximum bioaccumulation efficiency of 98% (Cd) and 43% (Cr). Biosensor bacteria are both environmentally sustainable and cost-effective. As a result, microbes have a range of metal absorption processes that allow them to have higher metal biosorption capabilities. Additionally, the biosorption potential of bacterium, fungus, biofilm, and algae, inherently handled microorganisms that immobilized microbial cells for the elimination of heavy metals, was reviewed in this study. Furthermore, we discuss some of the challenges and opportunities associated with producing effective heavy metal removal techniques, such as those that employ different types of nanoparticles.

Determination of total reducible organofluorine in PFAS-impacted aqueous samples based on hydrated electron defluorination.

Per- and polyfluoroalkyl substances (PFASs) is a large group of thousands of anthropogenic chemicals. Recently, measurement of total organic fluorine (TOF) to reflect the total PFASs has been recommended in limits and advisories. In this study, a total reducible organofluorine (TROF) assay is developed based on hydrated electron (eaq-) conversion of PFASs into inorganic fluorine combined with ion chromatograph, which is a common and widespread instrument. The eaq- is generated in UV/sulfite system with alkaline condition, and the concentration of TROF (CF_TROF) is the difference of fluoride concentration before and after assay. Method validation uses perfluorooctanesulfonic acid, perfluorooctanoic acid and their main alternatives, and F- recoveries are 76.6%-101%, except for perfluorobutanesulfonic acid (48.5%). Method application of TROF assay uses industrial surfactant products and fluorochemical industry-contaminated water, meanwhile, target PFAS analysis and total oxidizable precursors (TOP) assay are concurrently conducted. Concentrations of PFASs detected in target analysis and TOP assay were converted to fluorine equivalents concentrations (CF_Target and CF_TOP). ∑CF_Target and ∑CF_TOP account for 0.80%-36% of CF_TROF in industrial samples, 0.12%-54% in environmental water and 9.7%-14% in wastewater. The TROF assay can be used to initially judge whether PFASs contamination occurred near a hotspot with known sources. The CF_TROF could infer the extent of PFAS contamination in PFAS-impacted samples and estimate the fraction of uncharacterized PFAS.

Deciphering the impact of novel coronavirus pandemic on agricultural sustainability, food security, and socio-economic sectors-a review.

The Spanish flu, Asian flu, Hong Kong flu, HIV/AIDS, SARS, Ebola, and Swine flu, among others, have had a significant impact on agriculture, education, the economy, and human activities, including leisureliness, shipping, healthiness, fisheries, mining, industry, and trade. Currently, manhood is dealing with a new epidemic, the infection of the latest coronavirus (2019-nCoV), which causes a deadly disease named COVID-19. This article aims to examine COVID-19's effect on agriculture, education, and the economy. There are existing estimates to conclude that the COVID-19 pandemic has a significant influence on agriculture and the food supply chain, mostly influencing food demand and, as a result, food security, with a disproportionate impact on the most disadvantaged. To overcome spread of COVID-19, a non-contact food delivery system has been used by utilizing drown for this purpose. This epidemic crisis also introduced a digital education system that is challenging for students and teachers who are not educated in it. Weak infrastructure, such as electricity, poor access to the Internet connection, and a lack of technology literacy, has hampered the online education system. Coronavirus has an undesirable influence on the global economy by affecting tourism, the financial market, commerce, shipping, manufacturing, and the service sector. The exchange market was also down during the COVID-19 pandemic. In conclusion, we should strictly follow SOP's to improve our agriculture, education, economy, and other ways of normal life. We should also be vaccinated to fulfill our all losses in different fields.

Spatial trends and human health risks of organochlorinated pesticides from bovine milk; a case study from a developing country, Pakistan.

Bovine milk is a nutritious food commodity extensively produced and consumed in Punjab, Pakistan. This study assesses the concentration profile of organochlorine pesticides (OCP; 18 compounds) in buffaloes and cow's milk in eight major districts of Punjab, Pakistan and the potential impacts of such exposure. The total OCPs in buffaloes and cow's milk samples ranged from 3.93 to 27.63 ng mL-1 and 14.64-77.93 ng mL-1 respectively. The overall pattern of mean OCPs concentration in buffaloes and cows milk showed that Hexachlorocyclohexanes (HCHs) are predominant followed by Heptachlors and Dichlorodiphenyltrichloroethane (DDTs). So far, the concentration profile depicted that ∑HCHs, ∑DDTs and ∑Heptachlors did not exceed the maximum residual limits set for buffaloes and cow's milk. The spatial trends in terms of cluster analysis depicted significant variation (p > 0.05) among the districts in one cluster probably owing to local conditions. Furthermore, recently used DDTs were also identified at some of the selected districts. The risk assessment suggests that the estimated daily intake for each OCP was in accordance with the acceptable daily intake, thus single compound exposure does not pose a significant carcinogenic risk. However, the hazard ratios indicated that the values for ∑DDTs posed risk in adults consuming cow's milk whereas children may face carcinogenic risk on the consumption of both buffalo and cow's milk. The risk may be altered where mixture is considered, furthermore, regarding carcinogenic risks a continuous monitoring based ecological analysis is recommended in the future.

Assessment of organochlorine pesticides and health risk in tobacco farming associated with River Barandu of Pakistan.

Diffuse pesticide pollution through tobacco fields is a serious threat to both natural integrities and living beings because tobacco is known as a pesticide-intensive crop. Upsurge in the knowledge of detrimental impacts caused by organochlorine pesticides (OCPs) has made them a burning issue particularly in developing countries. Pakistan is a country famous for its agro-based economy and simultaneously is the second most significant pesticide consumer in South Asian countries. The studied area is tobacco hub of the country. Thus, the present work is aimed to investigate the contamination profile that highlights the ecological and health risk posed by OCPs in River Barandu, located in the proximity of tobacco farming region. ΣOCP levels in sediments ranged between 32.918 and 98.810 ng/g and in water between 0.340 and 0.935 µg/L. Hexachlorocyclohexanes (HCHs) and heptachlor were the most prevailing pesticides in both matrices of the river. Isomeric composition of DDTs and HCHs highlighted that the ß-HCH and p,p'-DDT were dominant isomers in water, while α-HCH and p,p'-DDT in sediment compartment. Enantiomeric compositions of HCH and DDT indicate both recent and historic uses of these compounds in the area. Indirect contamination through nearby tobacco clusters has been depicted through spatial analysis. Ecological risk assessment based upon the risk quotient (RQ) method revealed that α-endosulfan, dieldrin, heptachlor, and ∑HCHs represent a very high level of ecological risks. The OCPs' lifetime carcinogenic and non-carcinogenic health risks associated with dermal exposure to river's water were considered nominal for surrounding populations. However, detailed ecological and health risk studies are recommended considering the bio-accumulating nature of these contaminants in the food chain.

Polychlorinated biphenyls in indoor dust from urban dwellings of Lahore, Pakistan: Congener profile, toxicity equivalency, and human health implications.

This study is the pioneer assessment of the PCBs in indoor dust particles (from air conditioners) of an urbanized megacity from South Asian. The ∑35 PCB concentration ranged from 0.27 to 152.9 ng/g (mean: 24.84 ± 22.10 ng/g). The tri- and tetra-PCBs were dominant homologues, contributing 57.36% of the total PCB concentrations. The mean levels of Σ8 -dioxin-like (DL), Σ6 -indicator PCBs and WHO2005 -TEQ for DL-PCBs were 2.22 ± 2.55 ng/g, 9.49 ± 8.04 ng/g and 4.77 ± 4.89 pg/g, respectively. The multiple linear regression indicated a significant correlation of dusting frequency (p = 1.06 × 10-04) and age of the house (p = 1.02 × 10-06) with PCB concentrations in indoor environment. The spatial variation of PCB profile revealed relatively higher concentrations from sites near to illegal waste burning spots, electrical locomotive workshops, and grid stations. Human health risk assessment of PCBs for adults and toddlers through all three exposure routes (ie, inhalation, ingestion, and dermal contact) demonstrated that toddlers were vulnerable to high cancer risk (4.32 × 10-04 ), while adults were susceptible from low to moderate levels of risk (3.16 × 10-05 ). Therefore, comprehensive investigations for PCBs in the indoor settings, focusing particularly on the sensitive populations with relationship to the electronic devices, transformers, and illegal waste burning sites, are recommended.

Determination of total oxidizable precursors in foam surfactants and foam contaminated water based on UV-activated persulfate oxidation.

In this study, ultraviolet (UV)-activated persulfate under alkaline condition was developed as an alternative Total Oxidizable Precursor (TOP) assay to convert per- and polyfluoroalkyl substances (PFASs) precursors into detectable perfluoroalkyl acids (PFAAs). The conventional heat-based TOP assay takes 6 h at 85 °C, which is time consuming and may lose the volatile PFASs. Shorter time treatment would be beneficial to promote the analysis efficiency of the samples. We here report the UV-based TOP method as faster replacement of conventional heat-based TOP assay. The 6:2 fluorotelomer sulfonate (FTS), 8:2 FTS, and perfluorooctanesulfonamide (FOSA) achieved nearly complete conversion (with the molar yield of PFAAs of 97.2%-109.9%) at 60 min. This new UV-based TOP assay was then applied to 23 industry samples, and the results are comparable with the heat-based TOP assay. The perfluoroalkyl carboxylic acids (PFCAs) concentrations of 23 samples before and after normal TOP assay were 0-4290 mg L-1 and 438-77,420 mg L-1, respectively. The PFCAs after 60 min UV-based TOP assay was 310-81,881 mg L-1. The QuotientMol of 21 samples were 0.71-1.28, the QuotientMol of other two samples were 1.45 and 1.68, this probably due to the loss of volatile precursors during heat-based TOP assay. The UV-based TOP assay is an efficient, reliable and faster alternative to the conventional heat-based TOP assay.

Organochlorine pesticides (OCPs) in air-conditioner filter dust of indoor urban setting: Implication for health risk in a developing country.

This preliminary investigation highlights the occurrence of organochlorine pesticides (OCPs) in the indoor environment of a megacity, Lahore, Pakistan using the dust ensnared by air-conditioner filters. The Σ16 OCPs concentration ranged from 7.53 to 1272.87 ng/g with the highest percent contribution by ΣDDT (dichlorodiphenyltrichloroethane; 87.21%) and aldrin (6.58%). The spatial variation of OCPs profile revealed relatively higher concentration from homes near to agricultural and abandoned DDT manufacturing sites. Calculated isomer ratios revealed historic sources of hexachlorocyclohexanes (HCHs) and the fresh input of technical DDT and chlordane by the dwellers. The air conditioner dust was helpful to better understand the health risk in the indoor environment. So far a high lifetime cancer risk (10-3 ) was predicted for toddlers via accidental ingestion, inhalation, and dermal exposure. Similarly, the non-carcinogenic risk-based hazard quotient was found to be high for toddlers (6.94) and within the permissible limit (<1) for adults.

Spatial distribution of microplastics in soil with context to human activities: a case study from the urban center.

Plastic pollution is an emerging issue faced worldwide owing to rapid urbanization and extensive use of plastic products. The objective of this study was to determine and highlight the spatial distribution of microplastics (MPs) in the top soils of different land use types of an urbanized city, i.e., Lahore, Pakistan, influenced by different human activities. Soil samples (n = 40) were collected from eight different land use groups including agricultural areas, drains, dumping sites, industrial areas, lawns, parks, roadsides, and wastelands to represent the influence of corresponding human actions. After cleaning the samples through wet peroxide digestion and density separation, MPs were identified under a stereomicroscope. The range of MPs in top soils within Lahore district varied from 1750 to 12,200 MPs/kg with an average of 4483 ± 2315 MPs/kg. The highest concentration of MPs was present in the parks while the lowest numbers of MPs were enumerated in soil from dumping sites. About 99% of the identified MPs particles were fibers, sheets, and fragments in shape while foams and beads accounted for the rest. Moreover, among the size ranges, the large size MPs (300-5000 µm) were found in the majority (41.16%) followed by the fine size MPs (50-150 µm; 30.67%) and medium size MPs (150-300 µm; 28.17%). The current level of MPs pollution attracts the attention of researchers for a more comprehensive investigation in the future. It also incites the policy-makers to implement the regulations to prevent plastic pollution and its environmental implications.

An unintended challenge of microplastic pollution in the urban surface water system of Lahore, Pakistan.

Plastics are widely considered to be a major threat particularly in the urban areas owing to extensive use of plastic products. The current study is the first investigation to highlight the microplastics (MPs) pollution from the freshwater (Ravi River) located in the predominant urban center, i.e., Lahore, Pakistan. The concentration profile was quantified from surface water (n = 19) and sediments (n = 19) collected from different drains and canals of predominant freshwater resources in Lahore, Pakistan. The highest content of MPs was observed in the sullage carrier with mean concentration of 16,150 ± 80 MPs/m3 and 40,536 ± 202 MPs/m2 in the water and sediments respectively. The lowest level was detected in the link canals with mean concentration of 190 ± 141 MPs/m3 in the water and 683 ± 479 MPs/m2 in the sediments. The proportion of large size MPs (300 µm-5 mm) was maximum in the upstream section of Ravi river, whereas fine size MPs (50-150 µm) were dominant in the downstream section. In terms of shapes, the fragments were predominant with a relative abundance of 56.1% and 83.1% followed by fibers with a relative abundance of 38.6% and 11.8% in the water and sediments respectively. The chemical composition analysis showed that most of the fibers, fragments, and beads were polyethylene while the sheets were composed of polypropylene. Nevertheless, the foams isolated from the samples were composed of polystyrene. Within 24 h, about 2.4 ± 2.4 billion microplastic pieces were estimated to be transported from a single water channel into the river. The highest discharge of MPs was estimated from the sullage carrier with about 7 billion pieces/day.

Screening of human health risk to infants associated with the polychlorinated biphenyl (PCB) levels in human milk from Punjab Province, Pakistan.

This study assessed the polychlorinated biphenyl (PCB) levels in human milk and its associated health risk to infants from rural and urban settings of five districts of Punjab Province, Pakistan. The ∑34PCB concentrations ranged from 30.9 to 68.3 ng g-1 on lipid weight (l.w.) basis. The ∑8DL-PCB concentrations were ranged from 0.29 to 1.35 ng g-1 l.w., (mean 6.2 ± 8.7 ng g-1 l.w.), with toxicity equivalent to polychlorinated dibenzodioxins (PCDDs) ranging from 8.58 × 10-6 to 0.05 ng TEQ g-1 l.w. The spatial trend of PCB levels in human milk revealed higher bioaccumulative levels for urban mothers as compared with rural counterparts. The estimated daily intake (EDI) values of DL-PCBs to infants through trans-mammary transfer were considerably higher than tolerable daily intake limits established by WHO (i.e., 1-4 pg TEQ kg-1 bw) and other globally recognized organizations. Similarly, the hazard quotient values for TEQ ∑8DL-PCBs (range 1.21 to 79.87) were far above the benchmark value of 1 at all the sampling sites, indicating the high levels of adverse health risks to infants in the region through breast milk consumption. The ∑34PCB levels were found to be negatively correlated with mother' age (r = -0.31; p = 0.02), parity (r = - 0.85; p = 0.001), and infant' birth weight (r = - 0.73; p = 0.01). The present study suggests undertaking comprehensive public health risk assessment studies and firm regulatory measures to safeguard human health risks.

Organochlorine pesticides in the surrounding soils of POPs destruction facility: source fingerprinting, human health, and ecological risks assessment.

The elimination of persistent organic pollutants (POPs) obsolete pesticides stockpiles, particularly the organochlorine pesticides (OCPs), is one of the critical environmental issues faced by many developing countries. This pioneering study aimed to investigate the occurrence, source fingerprinting, human health, and ecological risks of OCPs in the surroundings of the lone POPs pesticide destruction facility in Pakistan. The ΣOCPs residual levels in soil ranged from 35.98 to 566.77 ng/g dry weight (dw), with a mean concentration of 174.42 + 111.62 ng/g (dw). The OCPs contamination levels in the soil followed the pattern as ΣHCHs > Σendrins > Σendosulfans > dieldrin > Σheptachlors > ΣDDTs > Σchlordanes > methoxychlor. The ΣHCHs residual concentrations were comparatively higher than the previous national and global soil studies. The recent accumulation of HCHs, DDTs, and heptachlor was observed in the study area as identified by ß-HCH/∑HCHs, (DDE + DDD)/ΣDDTs, heptachlor/Σheptachlor, and heptachlor exo-epoxide/heptachlor ratios. The OCPs' lifetime carcinogenic risk through ingestion, dermal, and inhalation exposure routes ranged from 1.65E-08 to 2.91E-07, whereas the noncarcinogenic hazard quotient (HQ) ranged from 9.12E-05 to 1.61E-03. The risk vulnerability among age groups was in the order: adult > toddler > child > teen > infant. The calculated risk levels were within an acceptable limit of one in a million (1 × 10-6) for carcinogenic risk and HQ < 1 for noncarcinogenic risk. The current OCPs residual levels, especially dieldrin and endrin, exhibited low to medium ecological risks when compared to various worldwide limits. The upsurge of the OCPs' environmental contamination levels over the years and consideration of the food chain transfer might amplify the human health and ecological risks intensities.

Degradation of PFOA Substitute: GenX (HFPO-DA Ammonium Salt): Oxidation with UV/Persulfate or Reduction with UV/Sulfite?

Hexafluoropropylene oxide dimer acid (HFPO-DA, ammonium salt with trade name: GenX) has been recently detected in river water worldwide. There are significant concerns about its persistence, and potential adverse effects to the biota. In this study, the degradability of GenX by typical advanced redox technologies (UV/persulfate and UV/sulfate) is investigated. Results demonstrate that <5% GenX is oxidized after 3 h in UV/persulfate system, which is much lower than ∼27% for PFOA. In comparison, GenX can be readily degraded and defluorinated by hydrated electron (eaq-) generated by UV/sulfite system. Specifically, GenX is not detectable after 2 h, and >90% of fluoride ion is recovered 6 h later. This is attributed to the accumulation and subsequent degradation of CF3CF2COOH and CF3COOH, which are stable intermediates of GenX degradation. Mechanistic investigations suggest that the etheric bond in the molecule is a favorable attack point for the eaq-. Such finding is corroborated by quantum chemical calculations. The side CF3- at the α-carbon probably acts as an effective barrier that prevents GenX from being cleaved by SO4-• or OH• at its most sensible point (i.e. the carboxyl group). This study illustrates that reduction by UV/sulfite might be a promising technology to remove GenX from contaminated water.

Quantification of polychlorinated biphenyl contamination using human placenta as biomarker from Punjab Province, Pakistan.

The present study biomonitored the placental polychlorinated biphenyl (PCB) concentrations in women from Punjab Province, Pakistan, that provides the pioneer data for occurrence and body burden of PCBs in placental tissues from South Asian women. The ∑34PCB concentrations in placental tissues were ranged from 20.2 to 115.98 ng/g lipid weight (lip. wt.), with predominance of tetra-PCB (54.67%). The levels of ∑8DL-PCBs and ∑6-indicator PCBs were ranged from 2.03 to 27.64 ng/g (lip. wt.) and 1.71 to 30.8 ng/g (lip. wt.), respectively. The WHO-TEQ2005 values for DL-PCBs were ranged from 1.18 × 10-5 to 0.067 ng/g (lip. wt.), with highest value evaluated for CB-126. The estimated daily intake (EDI) for DL-PCBs was ranged from 9.27 × 10-8 to 5.25 × 10-4 pg WHO-TEQ/kg body weight (bw), which was within the tolerable daily intake (TDI) values established by international organizations. The spatial distribution patterns of Σ34PCB concentrations from study area have shown relative higher concentrations in samples from urban and industrial cities than rural areas, and industrial and urban releases along with e-waste handling were recognized as vital PCB sources in the environment. In order to ascertain the transplacental transfer of PCBs, the fetal growth parameters were correlated with the ∑34PCB concentrations in placental tissues. The relationship between ∑34PCB concentrations in placental tissues and infant's anthropometric measures through multiple linear regression showed a negative correlation of infant's body weight (R2 = 0.0728), crown to heel length (R2 = 0.068), head circumference (R2 = 0.0342), chest circumference (R2 = 0.0001), and mid arm circumference (R2 = 0.0096) that noticeably highlights the inhibited fetal anthropometric development associated with maternal PCB bioaccumulation. Hence, an immediate elimination of ongoing PCB addition in the studied area has been emphasized and further investigations are suggested to appropriately manage the public and neonatal health risks in the region.

Risk profile and health vulnerability of female workers who pick cotton by organanochlorine pesticides from southern Punjab, Pakistan.

The present study was conducted to highlight the existing level of organochlorine-pesticides (OCPs) from human milk (n = 45) and blood serum (n = 40) of female workers who pick cotton in Khanewal District, southern Punjab, Pakistan. Source apportionment, congener-specific analysis, and risk surveillance of OCPs are reported from human milk and blood samples. Levels of OCPs in milk and blood serum samples ranged from 15.7 ppb to 538.3 ppb and from 16.4 ppb to 747.1 ppb, respectively, and were lower than previously published reports from other regions of the globe. Congener-specific analysis revealed that DDTs were predominant, followed by hexachlorocyclohexane, chlordane, and hexachlorobenzene. Calculated results for source apportionment analysis suggested that contamination load was a new input of DDTs as well as the historic use of lindane in the study area. Levels of OCPs in milk and blood serum were significantly (p < 0.05) correlated with age, time period of picking cotton, and number of children. Health risk revealed that female workers had risk of cancer among 1 per million; however, noncarcinogenic risks were not considerable. Environ Toxicol Chem 2017;36:1193-1201. © 2016 SETAC.

Polychlorinated biphenyl (PCBs) in rice grains and straw; risk surveillance, congener specific analysis, distribution and source apportionment from selected districts of Punjab Province, Pakistan.

The current study presents health risk surveillance by investigating the levels of polychlorinated biphenyls (PCBs) in rice (Oryza sativa L.) grains and rice straw. Samples were collected from four districts (Okara, Sahiwal, Lahore and Sheikhpura) of Punjab Province, Pakistan for congener specific analysis of PCBs, and to observe the spatial distribution pattern and point sources. Level of Σ30 PCB (ng g(-1)) in rice grains and rice straw ranged from 4.31 to 29.68 and 6.11-25.35, respectively. Tetra-CBs were found predominant in rice straw (49%) and grains (38%) over other PCB homologs. No significant variation (P>0.005) was observed for most of the screened PCBs congeners except, PCB-66, -77, -60, -101, -74, -138, -153 and -105 in rice grains and PCB-66 in rice straw. Reported toxicity equivalency (TEQ) values for dioxin like PCBs in rice grains were found lower than the previously published reports from Asian countries, however higher TEQ values are reported for rice straw in this study. Health was found at risk of cancer among one in million by consumption of the study area food stuffs, though no considerable carcinogenic risks to human health was found.

Human health risk assessment, congener specific analysis and spatial distribution pattern of organochlorine pesticides (OCPs) through rice crop from selected districts of Punjab Province, Pakistan.

To evaluate the screening level risk assessment of OCPs in rice (Oryza sativa L.) straw (n=20) and rice grains (n=20), samples were collected from different districts of Punjab Province, Pakistan. ∑OCPs' levels (ng g(-1)) in rice straw and grains ranged from 3.63 to 39.40, 2.72 to 49.89, respectively. DDTs were found predominant over the other detected OCP isomers followed by HCH and heptachlor. Results of one way ANOVA reflected no significant difference for OCPs' levels among sampling sites, except heptachlor for rice grains. ∑OCPs' concentration in rice straw samples was exceeding the minimal residual levels (MRLs) (Australian and Japanese). Results of dietary intake and risk assessment suggested that rice straw is not safe for animals to consume as fodder. Human health was suggested to have some carcinogenic risks by consumption of rice grains, however, no considerable hazardous risk (non-carcinogenic) to human health was found.